Bacterial agent capable of killing nematode and application thereof
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A technology of nematicides and fungicides, applied in the direction of nematicides, applications, biocides, etc., can solve problems such as no public reports
Inactive Publication Date: 2006-07-26
YUNNAN UNIV
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[0003] Through literature search, there is no pu
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Embodiment 1
[0046] Stenotrophomonas maltophilia G6 was inoculated on the test tube agar medium slope. The medium formula was: 2% yeast extract, 1.5% peptone, 2% glucose, 1.8% agar, the rest was water, and the pH was 7. The G6 mycelium was inoculated on the medium and cultured at 30°C for 2 days to obtain test tube seeds.
[0047] The test tube seed was inoculated into a 250ml Erlenmeyer flask liquid medium (100ml per bottle), the liquid medium formula was: 2% yeast powder, 15% soybean meal, 1% fish meal, 3% glucose, the rest was water, pH7. The culture time was 2 days in a shaker at 30°C, and the rotation speed was 220 rpm.
[0048]The fermentation culture was centrifuged at 8000 rpm / min for 20 minutes, the supernatant was taken, ammonium sulfate was added at a ratio of 1:0.85, and allowed to stand at 4°C for 2 hours, and then centrifuged at 8000 rpm / min for 30 minutes, and the supernatant was discarded. Re-dissolve with 10mM tris-hcl (pH 8.0), put the re-dissolved solution into 21mm dialysis...
Embodiment 2
[0050] It is basically the same as the first embodiment, except that the liquid culture medium formula is 3% yeast powder, 10% soybean meal powder, 2% fish meal, 1% glucose, and the rest is water.
Embodiment 3
[0052] It is basically the same as the first embodiment, except that the liquid culture medium formula is: 1% yeast powder, 20% soybean meal, 0.5% fish meal, 4% glucose, and the rest is water.
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Abstract
The invention relates to nematocide and its appliances thereof, belonging to the field of biological pesticide technique. Said nematocide is acquired through the sequence of culturing preparing strain in test tube, fluid enlargement culture and extracting nematocide active component, and said preparing strain is as follows: germinated barley-acceptable single cell bacteria stenotrophomonas maltophilia G6 which is preserved in microbe bacterial preservation management commission common microbe center of China with preservation data: 2005-10-31 and preservation registration number CGMCC No: 1522. The main mechanism of action of G6 strain is to break up body wall of worm through producing enzyme and poisoning worm, especially pine beam worm, and could be used to prepare biological nematocide. The production in said invention is characterized on high poison ability, which has better potential utility.
Description
Technical field: [0001] The invention relates to a fungus with nematicidal function and its application, and belongs to the technical field of microbial pesticides. Background technique: [0002] Plant parasitic nematodes are a kind of plant diseases that occur worldwide. Only root-knot nematodes have more than 70 known species, which damage more than 3,000 plants and cause huge losses every year. According to the statistics of the Food and Agriculture Organization of the United Nations, the annual global loss caused by nematodes is as high as 100 billion US dollars. In our country, nematodes mainly damage major economic crops such as tobacco, flowers, vegetables, cotton, soybeans, peanuts, panax notoginseng, and American ginseng, and have become an important limiting factor for the development of these crops. According to incomplete statistics from the National Information Center for Tobacco Pests and Diseases Control, Yunnan Province alone has an area of 27,000 hectares with ...
Claims
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Application Information
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