Rose containing flavone and malvidin, and method for production thereof

A rose and flavonoid technology, applied in biochemical equipment and methods, botanical equipment and methods, enzymes, etc., can solve problems such as unobtained

Active Publication Date: 2010-03-24
SUNTORY HLDG LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the roses that accumulate flavonoids, whether through hybridization or genetic recombination, have not obtained

Method used

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  • Rose containing flavone and malvidin, and method for production thereof
  • Rose containing flavone and malvidin, and method for production thereof
  • Rose containing flavone and malvidin, and method for production thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Example 1 : Simulation of the Cochrome Effect of Flavonoids on Anthocyanins

[0109] In order to simulate the co-coloring effect of flavonoids on anthocyanins, anthocyanins were first prepared. Cyanin is extracted and purified from the petals of the Chinese rose variety "Rote Rose" (rose cv. "Rote Rose"). Delphin is obtained from Verbena species "Tapien Violet" (vberbena cv. "Tapien Violet" or vberbena variety Sunmaref TP-V ("Tapien Violet") ("Tapien" is a Trade Mark registered in The pigment extracted from the petals of Japan)) is purified by alkaline hydrolysis. Malvin and Luteolin 7-O-glucoside were purchased from Funakoshi Co., Ltd., Japan.

[0110]To the various anthocyanins thus prepared, flavone (Luteolin 7-O-glucoside) was added at a molar concentration ratio of 0, 1, 2, and 4 equivalents in a pH 5.0 buffer, and the absorption spectrum was measured. As anthocyanins, Cyanin (Cyanidin 3,5-diglucoside (Cyanidin 3,5-diglucoside)), Delphin (Delphin 3, 5-Delph...

Embodiment 2

[0119] Embodiment 2 (reference example) : Introducing F3'5' from pansy to the rose variety "Lavande" H#40 Gene and Flavone Synthase Gene from Perilla

[0120] The plasmid pYFS3 containing the flavone synthase gene from perilla described in Japanese Patent Application Laid-Open No. 2000-279182, pYFS3 was digested with XbaI, blunted at the end, and further digested with BamHI to obtain perilla flavone synthase of about 1.8 kb Gene fragment. On the other hand, pSPB906 described in WO2005 / 017147 was digested with XhoI, blunted at the end, and further digested with BamHI. Between the blunt end and the BamHI cleavage site, the flavone synthase gene fragment of perilla was inserted to obtain 906-pYFS3. 906-pYES3 was inserted into the El 2 There is a perilla-derived flavone synthase gene between the 35S promoter and the D8 terminator (both described in WO2005 / 017147).

[0121] The BamHI and SalI sites of pSPB176 described in the report of Ueyama et al. (Ueyama et al. Plant Sci...

Embodiment 3

[0129] Example 3 : Introducing the F3'5'H#40 gene from Pansy into the rose variety "Lavande" and the flavone synthase gene from Butterfly

[0130] The Butterfly plant-derived flavone synthase gene described in the report of Akashi et al. (Plant Cell Physiol 40, 1182-1186, 1999) was inserted into the EcoRI and XhoI sites of plasmid pBluescript II SK(-), and the obtained plasmid was designated as pSPB426. After it was digested with KpnI, the end was blunted, and further digested with BamHI to obtain a flavone synthase gene fragment of about 1.7 kb from Butterfly. On the other hand, pSPB906 described in WO2005 / 017147 was digested with XhoI, blunted at the end, and further digested with BamHI. Between the blunt end and the BamHI cleavage site, the above-mentioned flavone synthase gene fragment of Butterfly grass was inserted to obtain 906-426.

[0131] The BamHI and SalI sites of pSPB176 described in the report of Ueyama et al. (Ueyama et al. Plant Science, 163, 253-263, 20...

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PUM

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Abstract

The invention provides a rose characterized by comprising a flavone and malvidin added by a genetic modification method. The flavone and malvidin are typically produced by expression of a transferred flavone synthase gene, pansy flavonoid 3',5'-hydroxylase gene and anthocyanin methyltransferase gene. The flavone synthase gene is, for example a flavone synthase gene of the family Scrophulariaceae,and specifically, the invention may be the flavone synthase gene of snapdragon of the family Scrophulariaceae, or the flavone synthase gene of torenia of the family Scrophulariaceae. The flavonoid 3',5'-hydroxylase gene is, for example, the pansy flavonoid 3',5'-hydroxylase gene. The anthocyanins methyltransferase gene is, for example, the methyltransferase gene of torenia of the family Scrophulariaceae.

Description

technical field [0001] The present invention relates to a rose artificially containing flavone and malvidin. The present invention relates to a method for changing the flower color of rose by genetically manipulating the co-pigmentation effect produced by containing flavonoids and delphinidin, especially a method for changing the flower color to blue. Background technique [0002] Flowers are the reproductive organs of plants and are necessary for the production of seeds for the next generation. In order to form a seed, pollen must attach to the pistil for fertilization. Usually pollen transport is performed by bees, insects such as butterflies, hummingbirds, and occasionally bats. The role of the petals is to lure these pollinators, and the plant manages to change color, shape, and pattern as a result. [0003] When appreciating flowers, flower color is the most important trait of flowers, so flowers of various colors have been bred through hybridization from ancient tim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09A01H5/00
CPCC12N9/1007C12N9/0073C12N9/0071C12N15/825C12Y114/11022C12Y114/13088C12Y201/01
Inventor 田中良和胜元幸久福井祐子水谷正子中村典子户上纯一
Owner SUNTORY HLDG LTD
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