Preparation method for high content macleaya cordata alkaloid

A high-concentration and high-content technology, applied in the field of high-content alkaloids, can solve the problems of difficulty in industrialized production, unavoidable waste acid water, high equipment requirements, etc., to accelerate dissolution, increase the processing capacity of production raw materials, and achieve low Effects of carbon production

Inactive Publication Date: 2015-12-09
长沙博拓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In addition to the above representative methods, there are methods such as ultrasonic extraction and acidic ethanol extraction, which either have high requirements for equipment and are temporarily difficult to implement industrial production, or the large consumption of ethanol leads to high cost and low product content. less desirable
In all the above methods, a large amount of acid wate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Take 300kg of raw material of boluo back fruit pods with the tested content, pulverize to 80-100 mesh, put it into the material metering tank of the atomization extraction equipment group, and pump the dilute sulfuric acid with pH 2-3 into the solvent tank of the equipment group to control the feed liquid. After about 2.5 hours, the pre-filtered extract is obtained; after the extract is centrifugally filtered by 800 mesh filter cloth, the AB- 8. Macroporous resin column adsorption, control the total alkaloid amount: the resin amount is 10:1 ~ 15:1; first rinse with purified water at a rate of 3 ~ 4BV / hour until the effluent is colorless; then use 85 ~ 90% The ethanol was eluted at a speed of 2BV / hour until the effluent was colorless, and the ethanol eluate was collected; the eluate was concentrated under reduced pressure and dried, and the total alkaloid was about 4.6kg, and the content was 66.85%, of which the blood The root alkali content is 45.56%, and the chelerythr...

Embodiment 2

[0040] Basically the same as Example 1, the difference is that the extraction solvent is dilute phosphoric acid with pH 2-3.

Embodiment 3

[0042] Basically the same as Example 1, the difference is that the extraction solvent is dilute hydrochloric acid with pH 2-3.

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PUM

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Abstract

The invention relates to a method for preparing total alkaloid from macleaya cordata and further separating the total alkaloid to obtain high content alkaloid, particularly a preparation method for high content sanguinarine and chelerythrine. The preparation method comprises: atomizing and extracting a macleaya cordata raw material by acid water, adsorbing and eluting an extraction liquid by weakly polar macroporous resin, and recovering a solvent from an eluant and drying the eluant to obtain the total alkaloid, the content of which is not less than 60%; dissolving the total alkaloid in low-degree ethanol, carrying out secondary adsorption by using polar macroporous resin, carrying out stepwise elution by using 60% and 80% ethanol, and recovering a solvent from an eluant and drying the eluant to separately obtain the chelerythrine and the sanguinarine, the contents of which are not less than 90%. The preparation method provided by the invention is simple in process step, short in production period, high in product yield and less in use level of solvent, the environment is slightly polluted, and the obtained product is high in content, stable in quality and low in cost.

Description

Technical field: [0001] The invention relates to a method for preparing total alkaloids from rhizoma rhizogenes and further separating them to obtain high-content alkaloids, in particular to a method for preparing high-content sanguinarine and chelerythrine. Background technique: [0002] Macleayacordata (willd) R.Br. belongs to the genus Papaveraceae and is widely distributed in my country. It contains benzylisoquinoline alkaloids, especially sanguinarine and chelerythrine. In the whole plant, the content of the pods is the highest, and the total alkaloid content can reach more than 1.5%. The raw material harvesting period begins in the middle and late August of each year. Bo Luohui total alkaloids have a wide range of pharmacological activities, mainly anti-tumor activity, antimicrobial activity and insecticidal activity, so it can be used to prepare agricultural, animal and human external insecticides, and can also be used to prepare anti-cancer. Under the current situati...

Claims

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Application Information

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IPC IPC(8): C07D491/153C07D491/056
CPCC07D491/153C07D491/056
Inventor 刘军刘芳邢健敏
Owner 长沙博拓生物科技有限公司
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