Preparation method for high content macleaya cordata alkaloid

Abstract

The invention relates to a method for preparing total alkaloid from macleaya cordata and further separating the total alkaloid to obtain high content alkaloid, particularly a preparation method for high content sanguinarine and chelerythrine. The preparation method comprises: atomizing and extracting a macleaya cordata raw material by acid water, adsorbing and eluting an extraction liquid by weakly polar macroporous resin, and recovering a solvent from an eluant and drying the eluant to obtain the total alkaloid, the content of which is not less than 60%; dissolving the total alkaloid in low-degree ethanol, carrying out secondary adsorption by using polar macroporous resin, carrying out stepwise elution by using 60% and 80% ethanol, and recovering a solvent from an eluant and drying the eluant to separately obtain the chelerythrine and the sanguinarine, the contents of which are not less than 90%. The preparation method provided by the invention is simple in process step, short in production period, high in product yield and less in use level of solvent, the environment is slightly polluted, and the obtained product is high in content, stable in quality and low in cost.

Description

Technical field: [0001] The invention relates to a method for preparing total alkaloids from rhizoma rhizogenes and further separating them to obtain high-content alkaloids, in particular to a method for preparing high-content sanguinarine and chelerythrine. Background technique: [0002] Macleayacordata (willd) R.Br. belongs to the genus Papaveraceae and is widely distributed in my country. It contains benzylisoquinoline alkaloids, especially sanguinarine and chelerythrine. In the whole plant, the content of the pods is the highest, and the total alkaloid content can reach more than 1.5%. The raw material harvesting period begins in the middle and late August of each year. Bo Luohui total alkaloids have a wide range of pharmacological activities, mainly anti-tumor activity, antimicrobial activity and insecticidal activity, so it can be used to prepare agricultural, animal and human external insecticides, and can also be used to prepare anti-cancer. Under the current situati...

AI Technical Summary

Problems solved by technology

[0008] In addition to the above representative methods, there are methods such as ultrasonic extraction and acidic ethanol extraction, which either have high requirements for equipment and are temporarily difficult to implement industrial production, or the large consumption of ethanol leads to high cost and low product content. less desirable

In all the above methods, a large amount of acid water (20-30 times or even higher by weight to the medicinal material) is used in the initial extraction process for long-term reflux or percolation extraction, or acid-base repeated treatment is used in the subsequent process ; The whole production process inevitably produces a large amount of waste acid water that needs to be treated, and the pressure on environmental protection is relatively high