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Application of chelerythrine in preparation of antifungal biofilm medicines

A chelerythrine and biofilm technology, which is applied in the field of medicine, can solve the problems that drugs that affect the prognosis of patients, antifungal activity do not have anti-biofilm activity, clinical treatment difficulty, etc., and achieve the effect of inhibiting growth and proliferation.

Inactive Publication Date: 2014-11-19
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, in vivo catheter or biological device-associated fungal infections that grow in the form of biofilms and exhibit high drug resistance pose great difficulties to clinical treatment, forcing clinicians to remove catheters or biodevices from patients while performing antifungal therapy. biodevices, thereby affecting patient outcomes
[0006] However, in the prior art, drugs with antifungal activity often do not have antibiofilm activity, resulting in that the expected effect cannot be obtained when it is used to treat fungi that grow in the form of biofilm

Method used

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  • Application of chelerythrine in preparation of antifungal biofilm medicines
  • Application of chelerythrine in preparation of antifungal biofilm medicines
  • Application of chelerythrine in preparation of antifungal biofilm medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] Embodiment 1: The effect of chelerythrine alone on the biofilm of different Candida albicans strains

[0080] Materials and methods

[0081] 1. Test drug

[0082] Chelidonium: purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0083] Fluconazole: purchased from Sigma Company.

[0084] Amphotericin B: purchased from Sigma Company.

[0085] XTT: purchased from Sigma Corporation.

[0086] Menadione (menadione): purchased from Sigma Company.

[0087] Dimethyl sulfoxide (DMSO): purchased from Sigma.

[0088] Acetone: purchased from Sigma Company.

[0089] Cheelerythrine was made into a 64mmol / L solution with dimethyl sulfoxide, amphotericin B was made into a 9.6mmol / L solution with dimethyl sulfoxide, and fluconazole was made into a 320mmol / L solution with dimethyl sulfoxide. Test drugs were stored at -20°C. Before the experiment, the drug stock solution was taken out and melted in a 35°C incubator, mixed thoroughly, and the p...

Embodiment 2

[0122] Example 2: Effect of chelerythrine alone on biofilms of different Cryptococcus neoformans strains

[0123] Materials and methods

[0124] 1. Test drug

[0125] Chelidonium: purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0126] Fluconazole: purchased from Sigma Company.

[0127] Amphotericin B: purchased from Sigma Company.

[0128] XTT: purchased from Sigma Corporation.

[0129] Menadione (menadione): purchased from Sigma Company.

[0130] Dimethyl sulfoxide (DMSO): purchased from Sigma.

[0131] Acetone: purchased from Sigma Company.

[0132] Cheelerythrine was made into a 64mmol / L solution with dimethyl sulfoxide, amphotericin B was made into a 9.6mmol / L solution with dimethyl sulfoxide, and fluconazole was made into a 320mmol / L solution with dimethyl sulfoxide. Test drugs were stored at -20°C. Before the experiment, the drug stock solution was taken out and melted in a 35°C incubator, mixed thoroughly, and the phar...

Embodiment 3

[0163] Embodiment 3: The effect of chelerythrine alone on the biofilm of different Aspergillus strains

[0164] Materials and methods

[0165] 1. Test drug

[0166] Chelidonium: purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0167] Fluconazole: purchased from Sigma Company.

[0168] Amphotericin B: purchased from Sigma Company.

[0169] XTT: purchased from Sigma Corporation.

[0170] Menadione (menadione): purchased from Sigma Company.

[0171] Dimethyl sulfoxide (DMSO): purchased from Sigma.

[0172] Acetone: purchased from Sigma Company.

[0173] Chelidonium was made into a 32mmol / L solution with dimethyl sulfoxide, amphotericin B was made into a 9.6mmol / L solution with dimethyl sulfoxide, and fluconazole was made into a 320mmol / L solution with dimethyl sulfoxide. Test drugs were stored at -20°C. Before the experiment, the drug stock solution was taken out and melted in a 35°C incubator, mixed thoroughly, and the pharmacod...

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Abstract

The invention relates to the technical field of medicine. Chelerythrine is isoquinoline-type alkaloid existing in plants such as celandine and macleaya cordata, and the structural formula of chelerythrine is shown in (I) described in the specification. The invention provides applications of chelerythrine in preparation of antifungal biofilm medicines, medical equipment or medical materials and the like. Experiments prove that chelerythrine has better antifungal biofilm activity both in vitro and in vivo, can effectively inhibit the growth and proliferation of fungal biofilms, and can effectively inhibit mature biofilms.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the application of chelerythrine in the preparation of antifungal biofilm drugs. Background technique [0002] Chelerythrine (chelerythrine) is the isoquinoline alkaloid that exists in plants such as celandine, Boluohui, etc., and its structural formula is as shown in (I), and molecular formula C 21 h 18 NO 4 + , molecular weight: 348.37, melting point 195-205°C. [0003] [0004] Chelidonium has rich medicinal value, can inhibit a variety of pathogenic bacteria, and has anti-tumor, insecticidal and other activities. Meng F from the Natural Medicine Research Center of Kunming General Hospital reported on the antifungal activity of chelerythrine against human pathogenic fungi (Meng F, Zuo G, Hao X, Wang G, Xiao H, Zhang J, Xu G. Antifungal activity of the benzo[c]phenanthridine alkaloids from Chelidonium majus Linn against resistant clinical yeast isolates.J Ethnopharmaco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/90A01P3/00A61K31/4741A61P31/10A61L31/16A61L15/44A61L29/16
Inventor 王彦胡淦海胡丹丹毕爽阎澜金磊姜远英
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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