Preparation method of macleaya cordata extracts

A Boluohui extract and Boluohui technology, applied in the field of preparation of Boluohui extract, can solve the problems of high cost, low extract content, large ethanol consumption, etc., and achieve improved utilization rate, high extraction rate, The effect of simple equipment

Active Publication Date: 2010-10-06
MICOLTA BIORESOURCE INC LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of method consumes a lot of ethanol, has hig

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Take 1000g of Boluohui fruit produced in Anhui, percolate with 10L of 0.5% sulfuric acid solution not lower than 80°C, add sodium hydroxide to the percolation solution to adjust pH=10 to alkali precipitation, and extract the obtained precipitate twice with 95% ethanol under reflux, For the first time, add 1 L of ethanol, add appropriate amount of activated carbon powder, and reflux for 2 hours; add 0.8 L of ethanol for the second time, add activated carbon powder, and reflux for 1 hour, combine the two ethanol extracts, concentrate and recover the ethanol to about 0.8 L while stirring Add concentrated sulfuric acid to the concentrated solution to adjust the pH=3, filter to obtain an orange-red precipitate, wash the precipitate with ethanol, and dry it to obtain 11.8 g of the extract. The total alkaloid content is 69.56% (the sanguinarine content is 46.54%, chelerythrine content is 23.02%).

Embodiment 2

[0048] Get 600g of Luohui fruit produced in Anhui, with a total alkaloid content of 1.68% (the content of sanguinarine is 1.13%, and the content of chelerythrine is 0.55%); another 400g of Luohui fruit produced in Guangxi, with a total alkaloid content of 1.14% (the content of sanguinarine is 0.73%, and the content of chelerythrine is 0.41%). The two raw materials are fully mixed, percolated with 20L of 0.5% hydrochloric acid solution not lower than 50°C, and hydrogen is added to the percolated liquid Potassium oxide adjusts pH=9 to alkali precipitation, and the resulting precipitate is extracted twice with ethanol reflux. For the first time, add 1 L of 90% ethanol, add activated carbon powder, and reflux for 2 hours; for the second time, add 0.8 L of 90% ethanol, add activated carbon powder, Reflux for 2 hours, combine the two ethanol extracts, concentrate and recover ethanol until the volume of the concentrated solution is about 0.8L, add concentrated hydrochloric acid to the...

Embodiment 3

[0050] Take 1000g of Boluohui fruit produced in Guangxi, percolate with 20L of 60°C 0.8% phosphoric acid solution, add calcium hydroxide to the percolation solution to adjust pH=10 to alkali precipitation, and reflux the obtained precipitate for extraction twice, add 1.5L of methanol for the first time, Add activated carbon powder, reflux for 2 hours; add methanol 1.2L for the second time, add activated carbon powder, reflux for 1 hour, combine the two alcohol extracts, concentrate and recover methanol until the volume of the concentrated solution is about 1L, add to the concentrated solution while stirring Concentrated phosphoric acid was adjusted to pH = 1, filtered to obtain an orange-red precipitate, the precipitate was washed with methanol, and dried to obtain 10.5 g of the extract. The total alkaloid content was 64.91% (the sanguinarine content was 44.77%, and The content of kalethrine is 20.14%).

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Abstract

The invention relates to a preparation method of macleaya cordata extracts, which comprises the following steps: carrying out diacolation on macleaya cordata medical materials by an acid solution; adding alkali into diacolation liquid for regulating the solution to the alkaline solution for carrying out alkali deposition; dissolving precipitates by low-carbon alcohol; adding a proper amount of active carbon for back flowing extraction; adding acid for generating salt after the recovery of partial solvents from extraction liquid, and separating out orange crystalline precipitates; filtering the precipitates; using alcohol to wash remained acid; and obtaining the macleaya cordata extracts after drying. The extracts have the main ingredients of benzophen anthridine alkaloid salt with sanguinarine and chelerythrine as the representatives, and the total content is not lower than 60 percent through being metered by raw alkali, wherein the content of the sanguinarine is not lower than 40 percent, and the content of the chelerythrine is not lower than 18 percent. The preparation of the macleaya cordata extracts of the invention has the advantages of simple method and equipment, stable process, short production period and little environment pollution. The obtained extracts have high yield, low cost and stable product quality.

Description

technical field [0001] The invention relates to a method for preparing a boluohui extract suitable for industrial production. technical background [0002] Macleaya cordata (Willd) R.Br. is a plant of the genus Macleaya cordata (Willd) R.Br., whose main active ingredients are benzylisoquinolines (benzylisoquinolines) alkaloids, mainly containing sanguinarine and celandine red Alkali, dihydrosanguinarine, dihydrochelerythrine and other benzophenanthridines (benzophenanthridines) alkaloids and protopines (protopines) alkaloids such as protopine and allocryptine, among which sanguinarine and The content of chelerythrine is high. The traditional method is to use the whole plant of Boluohui as medicine, but studies have found that the content of triphenanthridine alkaloids in the fruit of Boluohui is relatively high, which can reach more than 1.5%. It has been reported that the content of triphenanthridine alkaloids in the leaves of Boluohui can reach more than 1% in an appropr...

Claims

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Application Information

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IPC IPC(8): A61K36/66
Inventor 曾建国罗炼辉谈满良
Owner MICOLTA BIORESOURCE INC LTD
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