Anti-coronavirus macleaya cordata benzylisoquinoline alkaloid and resveratrol composition and application thereof
A technology of returning benzylisoquinoline and resveratrol, which is applied in antiviral agents, active ingredients of hydroxyl compounds, and medical preparations containing active ingredients, can solve problems such as low enzyme activity and difficult vaccine development, and achieve The effect of inhibiting infection
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Embodiment 1
[0045] Preparation of Boluohui benzylisoquinoline alkaloid and resveratrol composition virus infection inhibitor
[0046] The mass ratio of sanguinarine, chelerythrine, proopiate, allocryptine and resveratrol is uniformly mixed in a ratio of 1:0.1:0.1:0.1:1 to obtain a composition formulation.
[0047] Before the experiment, it was dissolved in PBS buffer and made into 2800 μg / ml (total concentration of sanguinarine, chelerythrine, protopine, allocryptine and resveratrol in the solution) and filtered. After sterilization, Store at -20°C for later use.
Embodiment 2
[0049] Cytotoxicity test
[0050] In this example, the neutral red phagocytosis method was used to measure the cytotoxicity of the composition formulation prepared in Example 1 to mammalian cells.
[0051] Table 1. The results of the cell maximum non-toxicity test of plant compound monomers and composition formulations
[0052]
Embodiment 3
[0053] Embodiment three, coronavirus infection inhibition test
[0054] In this example, the CPE method is used to measure the inhibitory effect of the composition formulation prepared in Example 1 on coronavirus infection. The tested coronavirus is murine hepatitis coronavirus A59 strain.
[0055] The results showed that both the compound formulation of the composition and ribavirin could interfere with coronavirus infection of 17Cl-1 cells. Among them, in the case of adding inhibitors at the same time as virus adsorption and before adsorption, the compound formulation of the composition had the best effect on inhibiting virus infection, and its virus half-inhibitory concentrations were 168±11 μg / ml and 129±16 μg / ml, respectively, while in Under the condition of adding inhibitors after virus adsorption, the control drug ribavirin has a better effect on inhibiting the virus, and its half inhibitory concentration of the virus is 243±25 μg / ml. Among monomeric inhibitors, when ...
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