Anti-coronavirus macleaya cordata benzylisoquinoline alkaloid and resveratrol composition and application thereof

A technology of returning benzylisoquinoline and resveratrol, which is applied in antiviral agents, active ingredients of hydroxyl compounds, and medical preparations containing active ingredients, can solve problems such as low enzyme activity and difficult vaccine development, and achieve The effect of inhibiting infection

Inactive Publication Date: 2020-04-07
金晓飞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As a highly pathogenic infectious virus, there is no clinically effective treatment for it. Most of them focus on symptomatic treatment and preventive intervention. Coronavirus is an RNA virus that is prone to mutations. , the enzyme activity of its error repair mechanism is very low, so it will mutate very quickly, and the vaccine is developed and researched based on the fixed gene or protein of the virus, so its vaccine is relatively difficult to develop. At present, it has been widely used Antiviral drugs such as: saquinavir, indinavir, ritonavir, nelfinavir, amprenavir and lopinavir ( lopinavir) is mainly used for human immunodeficiency virus (HIV), a pathogenic retrovirus that causes AIDS and its related diseases

Method used

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  • Anti-coronavirus macleaya cordata benzylisoquinoline alkaloid and resveratrol composition and application thereof
  • Anti-coronavirus macleaya cordata benzylisoquinoline alkaloid and resveratrol composition and application thereof
  • Anti-coronavirus macleaya cordata benzylisoquinoline alkaloid and resveratrol composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Preparation of Boluohui benzylisoquinoline alkaloid and resveratrol composition virus infection inhibitor

[0046] The mass ratio of sanguinarine, chelerythrine, proopiate, allocryptine and resveratrol is uniformly mixed in a ratio of 1:0.1:0.1:0.1:1 to obtain a composition formulation.

[0047] Before the experiment, it was dissolved in PBS buffer and made into 2800 μg / ml (total concentration of sanguinarine, chelerythrine, protopine, allocryptine and resveratrol in the solution) and filtered. After sterilization, Store at -20°C for later use.

Embodiment 2

[0049] Cytotoxicity test

[0050] In this example, the neutral red phagocytosis method was used to measure the cytotoxicity of the composition formulation prepared in Example 1 to mammalian cells.

[0051] Table 1. The results of the cell maximum non-toxicity test of plant compound monomers and composition formulations

[0052]

Embodiment 3

[0053] Embodiment three, coronavirus infection inhibition test

[0054] In this example, the CPE method is used to measure the inhibitory effect of the composition formulation prepared in Example 1 on coronavirus infection. The tested coronavirus is murine hepatitis coronavirus A59 strain.

[0055] The results showed that both the compound formulation of the composition and ribavirin could interfere with coronavirus infection of 17Cl-1 cells. Among them, in the case of adding inhibitors at the same time as virus adsorption and before adsorption, the compound formulation of the composition had the best effect on inhibiting virus infection, and its virus half-inhibitory concentrations were 168±11 μg / ml and 129±16 μg / ml, respectively, while in Under the condition of adding inhibitors after virus adsorption, the control drug ribavirin has a better effect on inhibiting the virus, and its half inhibitory concentration of the virus is 243±25 μg / ml. Among monomeric inhibitors, when ...

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Abstract

The invention discloses an anti-coronavirus macleaya cordata benzylisoquinoline alkaloid and resveratrol composition and application thereof. The macleaya cordata benzylisoquinoline alkaloid and resveratrol composition is prepared from five compositions including sanguinarine, chelerythrine, protopine, alpha-allocryptoxine and trans-resveratrol. The macleaya cordata benzylisoquinoline alkaloid andresveratrol composition has unique binding activity on a coronavirus related protein target, has a remarkable anti-coronavirus effect, and is expected to become a raw material of a drug for treatingpneumonia caused by human infection of Covid-19 coronavirus especially when concerning to the inhibition effect on Covid-19 coronavirus. The composition can be used for preparing medicines or other products for resisting diseases caused by coronavirus infection.

Description

technical field [0001] The invention relates to the fields of pharmacy and coronavirus infection drugs, in particular to a composition of anti-coronavirus benzylisoquinoline alkaloids and trans-resveratrol and its application. Background technique [0002] Plant compounds are called "the seventh type of nutrients", which widely exist in daily food and are a class of non-nutritive chemical substances that have special effects on human health. Relevant studies have found that some plant compounds have the biological activity of inhibiting virus infection, and have gradually become one of the hot spots in antiviral research. Preliminary research results show that the biological effects of plant compounds are mainly manifested in their impact on the biological characteristics of the cell membrane and changes in the balance of the local microenvironment. Functional flow and potential potential difference, and then play a certain biological effect. [0003] Macleaya cordata (Wil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/4741A61K31/395A61K31/4745A61K31/4375A61K31/05A61P31/14
CPCA61K31/4741A61K31/395A61K31/4745A61K31/4375A61K31/05A61P31/14A61K2300/00
Inventor 金晓飞陈鹏蔡媛
Owner 金晓飞
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