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Method for extracting high-purity sanguinarine and chelerythrine by using chromatographic technique

A technology of chelerythrine and sanguinarine, which is applied in the direction of organic chemistry and the like, can solve the problems of high cost, low purity, small sample loading, etc., and achieves simple and convenient operation, simple operation method, and solving the problem of loading sample. small effect

Active Publication Date: 2016-05-04
LISUI TECH SUZHOU
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Problems solved by technology

[0005] The object of the present invention is: in view of the above problems, provide a kind of method utilizing chromatographic technology to extract high-purity sanguinarine and chelerythrine, the method is simple to operate, low in energy consumption, low in cost, high in purity, and can obtain More than 98% of sanguinarine and chelerythrine, which not only overcomes the shortcomings of low purity prepared by traditional macroporous resin technology, but also solves the shortcomings of small sample loading and high cost in C18 reversed-phase technology

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  • Method for extracting high-purity sanguinarine and chelerythrine by using chromatographic technique
  • Method for extracting high-purity sanguinarine and chelerythrine by using chromatographic technique
  • Method for extracting high-purity sanguinarine and chelerythrine by using chromatographic technique

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Embodiment Construction

[0027] The above solution will be further described below in conjunction with specific embodiments. It should be understood that these examples are used to illustrate the present invention and not to limit the scope of the present invention. The implementation conditions used in the examples can be further adjusted according to the conditions of specific manufacturers, and the implementation conditions not indicated are usually the conditions in routine experiments.

[0028] The method for extracting hyperpurity sanguinarine and chelerythrine by using chromatographic technology disclosed in this implementation comprises the following steps:

[0029] 1) Pretreatment: After dissolving the Boluohui extract, filter it.

[0030] The Boluo solution obtained after filtration is in a clear and transparent state without solid impurities.

[0031] 2) Prepare buffer:

[0032] Use acetic acid to adjust the 20mol / ml ammonium acetate solution to pH = 5, then mix the pH-adjusted liquid wi...

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Abstract

The invention discloses a method for extracting high-purity sanguinarine and chelerythrine by using a chromatographic technique. The method comprises the following steps: (1) dissolving a macleaya cordata extract and then filtering; (2) preparing a corresponding buffer solution A and a corresponding buffer solution B; (3) injecting a macleaya cordata solution obtained through the filtering into a chromatographic column filled with SP-Sepharose FF fillers for adsorption; (4) flushing the chromatographic column by using the buffer solution A by 1 to 3 column volumes firstly, then eluting impurities in the chromatographic column by using the buffer solution B, and then eluting a target object from the chromatographic column by using the buffer solution B. The method provided by the invention is simple to operate, small in energy consumption, low in cost, and high in purity, and sanguinarine and chelerythrine with the purity above 98 percent can be obtained.

Description

technical field [0001] The invention relates to a method for extracting high-purity sanguinarine and chelerythrine, in particular to a method for extracting high-purity sanguinarine and chelerythrine by using a chromatography technique, and belongs to the technical field of pharmaceutical industry purification. Background technique [0002] Boluohui is a tall perennial herb belonging to the poppy branch Boluohui. It has many functions such as treating scabies, furunculosis, killing insects and maggots, etc. It has a long history in my country. The earliest document recording Boluohui is "Supplements to Materia Medica" , which is now uniformly named Boluohui, is widely used as a raw material for medicine, biopesticide, veterinary medicine, and fine chemical products. The main chemical components in Boluohui are alkaloids, with the highest content of sanguinarine and chelerythrine. [0003] The harm of chemical pesticides to people and the environment has prompted the pesticid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/153C07D491/056
CPCC07D491/056C07D491/153
Inventor 周胜张彦辉蔡吉
Owner LISUI TECH SUZHOU
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