Preparation method of oleuropein

A technology of oleuropein and an entraining agent, applied in the field of preparation of oleuropein, can solve the problems such as the industrialized production process disclosure of no extraction of oleuropein, and achieve the effects of being beneficial to large-scale production operations, low in energy consumption, and low in pollution

Inactive Publication Date: 2011-01-26
SUZHOU PAITENG BIOLOGICAL MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the prior art, there is no disclosure of an industrialized production process for extracting oleuropein

Method used

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  • Preparation method of oleuropein

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Experimental program
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Effect test

Embodiment 1

[0018] Get 10Kg of dried jasmine flowers and add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, and the volume percentage of the entrainer in the total extraction solvent is 4%, the extraction pressure is 50MPa, the temperature is 30°C, and the CO 2 The flow rate is 10L / min, the extraction time is 100min, the extract is obtained, dissolved in water, extracted once with an equal amount of ethyl acetate, the water layer is taken, adsorbed by an AB-8 macroporous adsorption resin column, eluted with 35% ethanol, and collected for 3 The eluate was twice the volume of the column, the ethanol was recovered under reduced pressure and concentrated, refrigerated for 12 hours, filtered, the filter residue was taken, recrystallized by adding absolute ethanol, separated and crystallized, washed and dried to obtain 253.3g of oleuropein, which was detected by HPLC , with a purity of 98.2%.

Embodiment 2

[0020] Get 10Kg of dried jasmine flowers and add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, and the volume percentage of the entrainer in the total extraction solvent is 6%, the extraction pressure is 70MPa, the temperature is 70°C, and the CO 2 The flow rate was 30L / min, and the extraction time was 250min. The extract was obtained, dissolved in water, extracted three times with an equal amount of ethyl acetate, and the water layer was taken, adsorbed by an AB-8 macroporous adsorption resin column, eluted with 35% ethanol, and collected 8 The eluate was twice the volume of the column, the ethanol was recovered under reduced pressure and concentrated, refrigerated for 36 hours, filtered, the filter residue was taken, recrystallized by adding absolute ethanol, separated and crystallized, washed and dried to obtain 389.0 g of oleuropein, which was detected by HPLC , with a purity of 97.3%.

Embodiment 3

[0022] Get 10Kg of dried jasmine flowers and add to CO 2 In the supercritical extractor, ethanol is used as the entrainer, and the volume percentage of the entrainer in the total extraction solvent is 5%, the extraction pressure is 60MPa, the temperature is 50°C, and the CO 2 The flow rate is 20L / min, and the extraction time is 180min. The extract is obtained, dissolved in water, extracted 1-3 times with an equal amount of ethyl acetate, and the water layer is taken, adsorbed by an AB-8 macroporous adsorption resin column, and eluted with 35% ethanol. Collect the eluate with 3-8 times the column volume, recover the ethanol under reduced pressure and concentrate, refrigerate for 12-36 hours, filter, take the filter residue, add absolute ethanol for recrystallization, separate the crystals, wash and dry to obtain oleuropein 307.2g, detected by HPLC, the purity is 98.9%.

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Abstract

The invention relates to a preparation method of oleuropein, which has the characteristics of convenient operation and less pollution and energy consumption and comprises the following process steps of: taking dries jasminum polyanthum flowers, adding into a CO2 supercritical extractor to obtain an extract by using ethanol as an entrainer, adding water for dissolving, extracting 1-3 times with an equal quantity of ethyl acetate, taking a water layer, adsorbing through an AB-8 macroporous absorbent resin column, eluting with 35-percent ethanol, collecting an eluant accounting for 3-8 times the column volume, recovering the ethanol through decompression, concentrating, refrigerating for 12-36 hours, filtering, taking filtering residues, adding anhydrous ethanol for recrystallization, separating crystals, washing and drying. The oleuropein prepared by adopting the invention is high in product purity and easy for industrialized amplification.

Description

technical field [0001] The invention relates to a preparation method of oleuropein, in particular to a preparation method of extracting oleuropein from plants. Background technique [0002] Oleuropein (Oleuropein), molecular formula: C25H32O13, molecular weight: 540.52, CAS accession number: 32619-42-4, mainly exists in various plants of Oleaceae, among which Jasminum polyanthum Franch. . Its molecular formula is as follows: [0003] [0004] Oleaceae plant Jasminum polyanthum Franch. The perianth is used as a traditional Chinese medicine Suxinghua, which has the effects of promoting qi, promoting blood circulation, relieving pain, clearing away heat and dissipating stagnation. [0005] Modern studies have shown that oleuropein has various pharmacological effects such as antibacterial, anti-inflammatory, antihypertensive, and insecticidal, and it is also a raw material for the preparation of other important active compounds. [0006] In the prior art, there is no discl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08
CPCY02P20/54
Inventor 王峰王琳
Owner SUZHOU PAITENG BIOLOGICAL MEDICAL TECH
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