Epidermal growth factor compound lipidosome as well as preparation method and application thereof

A technology of epidermal growth factor and compound, which is applied in the direction of skin care preparations, pharmaceutical formulas, cosmetic preparations, etc., can solve the problem that the skin care and skin health effects of epidermal growth factor cannot be fully exerted, active substances are easily affected by external conditions, and cannot Prevent skin aging and other problems, achieve the effect of preventing bad cell growth, maintaining cell elasticity, clearing away heat and draining skin fatigue

Active Publication Date: 2014-01-15
GUANGZHOU GIALEN COSMETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, traditional cosmetics only supplement the external nutrition of the skin and cover the aging skin, and cannot prevent the aging of the skin
Moreover, the actual effect of the nutrients and active cosmetics produced by the general method is not large, because the nutrients and actives are not easy to penetrate into the deep layer of the skin. Normal skin can only absorb some small molecules and fat-soluble substances, and cannot absorb macromolecular proteins. It is more difficult to enter the cell, and the active substance is often inactivated when it is used because it is easily affected by external conditions
As a polypeptide and protein, epidermal growth factor (EGF) is sensitive to enzymes, light, pH, etc., and has the characteristics of poor stability, easy inactivation, and low bioavailability, which greatly limits its application. In order to solve the above problems, Many cosmetics simply encapsulate epidermal growth factor in liposomes to form a single epidermal growth factor liposome for use. Although the effects of enzymes and pH values ​​are isolated and the activity of epidermal growth factor is protected to a certain extent, the use of The rate is still unsatisfactory, and the skin care and skin health effects of epidermal growth factor cannot be fully exerted

Method used

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  • Epidermal growth factor compound lipidosome as well as preparation method and application thereof
  • Epidermal growth factor compound lipidosome as well as preparation method and application thereof
  • Epidermal growth factor compound lipidosome as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031](1) Dissolve 30mg of hydrogenated lecithin, 10mg of cholesterol, 5mg of silibinin and 5mg of coix seed oil in 50ml of absolute ethanol to prepare a membrane material solution; 0.1mg of epidermal growth factor, 5mg of yeast extract, 5mg of microbial polysaccharide Dissolve 5mg glycerol in 10ml phosphate buffer (PH6.8) to prepare EGF solution;

[0032] (2) Put the above-mentioned membrane material solution and EGF solution into a supercritical autoclave, seal the supercritical autoclave, and feed CO2 through a carbon dioxide high-pressure pump 2 , emulsified at a pressure of 10Mpa and a temperature of 30°C for 20 minutes, CO 2 The flow rate is 15L / h; the emulsified product is separated under reduced pressure, the separation temperature is 30°C, and the separation pressure is 5Mpa; the separated emulsified product is collected to obtain the epidermal growth factor complex liposome I.

Embodiment 2

[0034] (1) Dissolve 50mg of hydrogenated lecithin, 15mg of cholesterol, 10mg of silibinin and 10mg of coix seed oil in 50ml of absolute ethanol to prepare a membrane material solution; 0.5mg of epidermal growth factor, 8mg of yeast extract, 8mg of microbial polysaccharide Dissolve 10mg glycerol in 10ml phosphate buffer (PH6.8) to prepare EGF solution;

[0035] (2) Put the above-mentioned membrane material solution and EGF solution into a supercritical autoclave, seal the supercritical autoclave, and feed CO2 through a carbon dioxide high-pressure pump 2 , emulsified at a pressure of 15Mpa and a temperature of 35°C for 25 minutes, CO 2 The flow rate is 15 L / h; the emulsified product is separated under reduced pressure, the separation temperature is 35° C., and the separation pressure is 6 MPa; the separated emulsified product is collected to obtain the epidermal growth factor complex liposome II.

Embodiment 3

[0037] (1) Dissolve 80mg of hydrogenated lecithin, 25mg of cholesterol, 12mg of silibinin and 20mg of coix seed oil in 50ml of absolute ethanol to prepare a membrane material solution; mix 1mg of epidermal growth factor, 10mg of yeast extract, 10mg of microbial polysaccharide and Dissolve 15mg glycerol in 10ml phosphate buffer (PH6.8) to prepare EGF solution;

[0038] (2) Put the above-mentioned membrane material solution and EGF solution into a supercritical autoclave, seal the supercritical autoclave, and feed CO2 through a carbon dioxide high-pressure pump 2 , emulsified at a pressure of 15Mpa and a temperature of 40°C for 30 minutes, CO 2 The flow rate is 15L / h; the emulsified product is separated under reduced pressure, the separation temperature is 40°C, and the separation pressure is 8Mpa; the separated emulsified product is collected to obtain the epidermal growth factor complex liposome III.

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Abstract

The invention discloses epidermal growth factor compound lipidosome as well as a preparation method and application thereof, and belongs to the field of traditional Chinese medicines. The epidermal growth factor compound lipidosome is prepared by the following raw materials in parts by mass: 0.1-5 parts of epidermal growth factors, 5-30 parts of silibinin, 5-30 parts of coix seed oil, 5-30 parts of yeast extracts, 5-30 parts of microbial polysaccharide, 30-200 parts of hydrogenated lecithin, 10-80 parts of cholesterol and 5-30 parts of glycerinum. The epidermal growth factor compound lipidosome has the advantages of simple technique, safety, no poison, little skin irritation, mild property and stable effect.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to an epidermal growth factor complex liposome and a preparation method and application thereof. Background technique [0002] At present, traditional cosmetics only supplement the external nutrition of the skin and cover the aging skin, and cannot prevent the aging of the skin. Moreover, the actual effect of the nutrients and active cosmetics produced by the general method is not large, because the nutrients and actives are not easy to penetrate into the deep layer of the skin. Normal skin can only absorb some small molecules and fat-soluble substances, and cannot absorb macromolecular proteins. It is more difficult to enter the cells, and the active substances are often inactivated when they are used because they are easily affected by external conditions. As a polypeptide and protein, epidermal growth factor (EGF) is sensitive to enzymes, light, pH, etc., ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/97A61K8/92A61K8/73A61K8/64A61K8/49A61K8/14A61Q19/00A61Q19/02A61Q19/08A61Q1/02
Inventor 侯秋红
Owner GUANGZHOU GIALEN COSMETICS
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