Reducing enzyme of nitryl, phenylnitryl, encoding gene and application

Abstract

This invention describes the coding gene and applications of a nitrobenzene nitro-reducing enzyme, comprising a protein from the following amino acid residue sequences: 1) SEQ ID No. 1 in the sequence table; 2) the amino acid residue sequence in SEQ ID No. 1 is a protein substituted, deleted or added with 1-10 amino acid residues and having the nitrobenzene nitro-reducing enzyme function. The nitrobenzene nitro-reducing enzyme and its coding gene in this invention play an important role in the production of ortho-aminophenols and their derivatives.

Description

technical field

[0001] The invention relates to a nitrophenyl nitroreductase and its coding gene and application in the technical fields of microbial biotechnology and genetic engineering. Background technique

[0002] my country has become the world's largest producer of antipyretic and analgesic drugs. The output of aspirin, paracetamol, and analgin exceeds 10,000 tons, and the output of phenacetin, aminopyrine, and antipyrine exceeds 1,000 tons. Ton. At present, the output of antipyretic and analgesic drugs in my country is growing rapidly, and it is expected to increase at a rate of about 8% per year in the future. With the growth of antipyretic and analgesic drugs, their intermediates have also achieved considerable development. In 2003, domestic paracetamol consumption increased rapidly, and exports also showed a rapid growth momentum. The export volume was 28,163 tons, and the annual export volume increased by about 1 times compared with the previous year. In 2004, ...