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A kind of synthetic method of chlorothalonil artificial antigen

A technology of artificial antigen and synthesis method, applied in chemical instruments and methods, hybrid peptides, ovalbumin, etc., can solve the problems of cumbersome operation, inability to realize rapid detection of a large number of samples, high cost, etc., and achieve the effect of simple synthesis steps

Active Publication Date: 2015-10-28
JIANGNAN UNIV
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Problems solved by technology

Chlorothalonil has no systemic conduction effect, but once it is sprayed on the plant body, it can have good adhesion on the surface of the plant and is not easily washed away by rain, so the drug has a long period of efficacy. In addition, it is widely used , therefore, chlorothalonil and its metabolites have relatively large residues in plants and soil, and are even detected in arctic regions
At present, the methods for detecting chlorothalonil are mainly gas chromatography (GC) and liquid chromatography (HPLC) and other instrumental methods, but these methods have the disadvantages of cumbersome operation, time-consuming, expensive and other shortcomings, and cannot realize the rapid detection of a large number of samples , so it is of great significance to establish a fast and simple method for the detection of chlorothalonil

Method used

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  • A kind of synthetic method of chlorothalonil artificial antigen
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  • A kind of synthetic method of chlorothalonil artificial antigen

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Embodiment 1

[0032] (1) Synthesis of hapten: Weigh 500mg (1.9mmol) of chlorothalonil original drug, KOH 106.6mg (1.9mmol), 262mg (2mmol) of 6-aminocaproic acid, and dissolve these three drugs in 20mL of ethanol at 60℃ React for 12h, then at 90°C for 12h. The reactant was rotary evaporated, dissolved in 20 mL of water, adjusted to pH 4-5 with hydrochloric acid, and precipitated, which was dried to form the hapten CTNH. Use liquid-mass combined technology for identification and analysis.

[0033] (2) Preparation of conjugate CTNH-BSA

[0034] Weigh 35.8 mg of CTNH, 56.9 mg of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), 35.6 mg of N-hydroxysuccinimide (NHS), and use 1 mL Anhydrous dimethylformamide (DMF) was dissolved (referred to as liquid A), and the reaction was stirred at room temperature for 8 hours. Weigh 112 mg of bovine serum albumin (BSA) and dissolve it in 20 mL of 0.01mol / L pH7.2 PBS (called solution B), add solution A to solution B drop by drop at room temperature, and rea...

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Abstract

The invention discloses a method for synthesis of a chlorothalonil artificial antigen and belongs to the technical field of biochemical engineering. A chlorothalonil raw drug and 6-aminocaproic acid undergo a reaction to produce hapten CTNH as a hydroxy product, the CTNH is coupled with carrier protein bovine serum albumin (BSA) by a carbodiimide method so that a conjugate CTNH-BSA is obtained and is used as an immunizing antigen, the CTNH is coupled with carrier protein chicken egg albumin (OVA) so that a conjugate CTNH-OVA is obtained and is used as a coating antigen, and conjugate coupling ratios are determined by an ultraviolet method. The method realizes successfully synthesis of the chlorothalonil artificial antigen, has simple and effective synthesis steps, can be completely used for immunization analysis and provides a necessary artificial antigen for later research.

Description

Technical field [0001] A method for synthesizing chlorothalonil artificial antigen belongs to the technical field of biochemical industry. Background technique [0002] Chlorothalonil is a broad-spectrum organochlorine bactericide, which has a preventive effect on various crops and various green lawns. Chlorothalonil has no systemic conduction effect, but once it is sprayed on the plant body, it can have good adhesion on the body surface and is not easy to be washed away by rain, so the efficacy period is longer, and it is widely used Therefore, chlorothalonil and its metabolites have large residues in plants and soil, even in the Arctic. The current methods for detecting chlorothalonil are mainly instrument methods such as gas chromatography (GC) and liquid chromatography (HPLC), but these methods have disadvantages such as cumbersome operation, time-consuming, and relatively expensive cost, and cannot realize rapid detection of a large number of samples. Therefore, it is of g...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/77C07K14/765G01N33/68G01N33/53
CPCC07K14/765C07K14/77C07K19/00G01N33/68G01N2430/00
Inventor 匡华胥传来严会娟刘丽强徐丽广马伟宋珊珊
Owner JIANGNAN UNIV
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