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Synthetic method of perfluorooctanoic acid medicament universal artificial antigen

A technology of artificial antigen and perfluorooctanoic acid, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., to achieve the effect of concise synthesis steps

Inactive Publication Date: 2008-12-24
JIANGNAN UNIV
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AI Technical Summary

Problems solved by technology

So far, there is no report on the immunoassay method for perfluorooctanoic acid PFOA at home and abroad. In order to make up for this gap, a complete antigen for the detection of perfluoroorganic substances is designed and synthesized directly using perfluorooctanoic acid (Perfluorooctanoic acid) as a hapten.

Method used

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  • Synthetic method of perfluorooctanoic acid medicament universal artificial antigen

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Embodiment 1

[0012] (1) Preparation of artificial antigen

[0013] Weigh 2 mg (0.00483 mmol) of perfluorooctanoic acid and 8 mg (0.000121 mmol) of bovine serum albumin BSA and dissolve them in 1 mL of PBS (0.1 mol / L) solution. After fully dissolving, add the methanol solution of water-soluble carbodiimide EDC drop by drop. (40 μL EDC dissolved in 50 μL methanol), stirred gently at room temperature for 3 hours. That is, the conjugate PFOA-BSA mixture is obtained.

[0014] Dialysis bag pre-treatment: Take a 10cm dialysis bag, boil it in boiling water for 5 minutes, rinse it with 60°C deionized water for 3 minutes, and store it in 4°C deionized water for later use.

[0015] The reaction solution was put into a dialysis bag and dialyzed in 0.01mol / L PBS for 3 days, and the solution was changed three times a day.

[0016] (2) Identification of PFOA artificial antigen

[0017] The determination of the coupling ratio, that is, the method for estimating the ratio of the two molecules coupled in...

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Abstract

The invention relates to a method for synthesizing a genetic artificial antigen for perflurooctanoic acid medicines, which belongs to the biochemical technical field. The method takes perflurooctanoic acid PFOA as a hapten, couples the perflurooctanoic acid PFOA with a carrier protein, namely bovine serum albumin BSA by utilization of carbodiimide, and determines the coupling ratio of a coupling object by an ultraviolet scanner. The method successfully synthesizes the artificial antigen of the perflurooctanoic acid, namely perflurooctanoic acid-bovine serum albumin, has concise and effective synthetic procedures, can be completely used for immunoassay, provides a necessary artificial antigen for further research of people, and can meet the demand of domestic research of the artificial antigen.

Description

technical field [0001] The invention discloses a method for synthesizing a general artificial antigen of perfluorooctanoic acid drugs, which belongs to the field of biochemical technology. Background technique [0002] Perfluorooctanoic acid, English name: Perfluorooctanoic acid (PFOA), CAS number 335-67-1, molecular formula C 8 HF 15 o 2 . Perfluorooctanoic acid (PFOA) and its salts are commonly used fluorosurfactants, which have strong hydrophobicity and oleophobicity, and their chemical structure is far more stable than other surfactants. Due to its excellent heat resistance, low temperature resistance, self-lubricating property and chemical stability, etc., it is used in the fields of aerospace, electronics, chemistry and fire protection, and is used in life departments such as non-stick cookware, waterproof and breathable materials (such as waterproof clothing) ), leather, auto parts and microwave popcorn bags, etc. PFOA is difficult to degrade and has accumulation...

Claims

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Application Information

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IPC IPC(8): C07K14/765C07K1/14C07K1/10
Inventor 胥传来李灼坤彭池方马伟徐丽广袁媛谢会玲
Owner JIANGNAN UNIV
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