Synthetic method of perfluorooctanoic acid medicament universal artificial antigen
A technology of artificial antigen and perfluorooctanoic acid, which is applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., to achieve the effect of concise synthesis steps
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Embodiment 1
[0012] (1) Preparation of artificial antigen
[0013] Weigh 2 mg (0.00483 mmol) of perfluorooctanoic acid and 8 mg (0.000121 mmol) of bovine serum albumin BSA and dissolve them in 1 mL of PBS (0.1 mol / L) solution. After fully dissolving, add the methanol solution of water-soluble carbodiimide EDC drop by drop. (40 μL EDC dissolved in 50 μL methanol), stirred gently at room temperature for 3 hours. That is, the conjugate PFOA-BSA mixture is obtained.
[0014] Dialysis bag pre-treatment: Take a 10cm dialysis bag, boil it in boiling water for 5 minutes, rinse it with 60°C deionized water for 3 minutes, and store it in 4°C deionized water for later use.
[0015] The reaction solution was put into a dialysis bag and dialyzed in 0.01mol / L PBS for 3 days, and the solution was changed three times a day.
[0016] (2) Identification of PFOA artificial antigen
[0017] The determination of the coupling ratio, that is, the method for estimating the ratio of the two molecules coupled in...
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