Preparation method for artificial antigen of erythrosin pigment

An artificial antigen, erythrosine technology, applied in the preparation method of peptides, chemical instruments and methods, animal/human proteins, etc., to achieve the effect of concise synthesis steps

Active Publication Date: 2012-04-11
MEIZHENG BIO TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the immunological detection method for erythrosine structural residues at home and abroad. For this purpose, a complete antigen for the detection of erythrosine artificial synthetic pigments was designed and synthesized using the erythrosine derivative ETS as a hapten.

Method used

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  • Preparation method for artificial antigen of erythrosin pigment
  • Preparation method for artificial antigen of erythrosin pigment
  • Preparation method for artificial antigen of erythrosin pigment

Examples

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Embodiment 1

[0022] (1) Preparation of hapten

[0023] Take 88mg (0.1mmol) of erythrosine and 0.11mmol of hydrochloric acid and react in the dark for 6h in an aqueous solution, centrifuge to get the precipitate and dilute it with N,N-dimethylformamide DMF, then add 0.5mL of 0.1mmol hexaaminocaproic acid and 0.15 mmol carbodiimide EDC and 0.15 mmol N-hydroxysulfosuccinimide Sulf-NHS were reacted in the dark for 24 hours, and the supernatant was collected by centrifugation to obtain the erythrosine derivative ETS.

[0024] (2) Preparation of artificial antigen

[0025] Take 0.5 mL of N,N-dimethylformamide DMF solution containing 20 mg (0.02398 mmol) of erythrosine derivative ETS into a brown vial, and then add 15.52 mg (0.06807 mmol) of N-hydroxysulfosuccinimide Sulf -NHS and 17.32mg (0.09033 mmol) carbodiimide EDC, reacted at room temperature for 8h, and it was liquid A. Dissolve 32.5mg (0.0005mmol) of bovine serum albumin BSA into 0.01M, pH7.4 phosphate PBS buffer solution, which is liquid...

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Abstract

The invention discloses a preparation method for artificial antigen of erythrosin pigment and belongs to the technical field of biochemical engineering. Erythrosin derivant ETS is used as a hapten and is conjugated with a carrier protein-BSA (Bovine Serum Albumin) through an active ester method to obtain the artificial antigen of erythrosine which is erythrosin derivant ETS-BSA; and the conjugating ratio of a conjugate is determined through an ultraviolet scanner. The preparation method for artificial antigen of erythrosin pigment can be used for successfully synthesizing the antigen of erythrosin derivant ETS, has simple and effective synthesis steps, can be completely used in immunoassay, provides necessary artificial antigen for further study of people and can satisfy the domestic study demands on the artificial antigen.

Description

technical field [0001] The invention discloses a method for preparing an artificial antigen of erythrosin, which belongs to the technical field of biochemical industry. Background technique [0002] Erythrosine, the English name is: Erythrosine, and its chemical name is: spiroisobenzofuran-1(3H),9'-[9H]xanthene-3-one-3',6'-dihydroxy-2' ,4',5',7'-tetraiododisodium salt, also known as tetraiodofluorescein B, CAS No. 568-63-8, molecular formula is C 20 h 6 I 4 Na 2 o 5 . Erythrosine is a kind of synthetic pigment with bright color, which is often used in food processing products and beverages to improve its sensory properties. Food colorants can be divided into two categories: natural pigments and synthetic pigments. Synthetic pigments are widely used in the food processing industry because of their low cost, diverse tones, bright colors and strong tinting strength. Because synthetic pigments use chemical products such as benzene, toluene, and naphthalene as raw material...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K1/113
Inventor 王利兵胥传来李灼坤
Owner MEIZHENG BIO TECH CO LTD
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