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Preparation method of artificial antigen of 4-aminobenzophenone

An aminobenzophenone, artificial antigen technology, applied in the direction of animal/human protein, albumin peptide, serum albumin, etc., can solve the problems of high cost, can not meet the requirements of fast, convenient and accurate, and achieve simple synthesis steps Effect

Inactive Publication Date: 2010-09-15
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of benzophenone and 4-aminobenzophenone at home and abroad mainly includes high-performance liquid chromatography (HPLC), gas chromatography (GC), gas-mass spectrometry (GC-MS), liquid- Mass spectrometry (LC-MS), etc., but these methods not only require expensive instruments and equipment, professional operators, but also have relatively high requirements for test materials, and further sample pretreatment is required, which can no longer meet modern detection Requirements for fast, convenient and accurate
At present, there is no report of benzophenone ELISA at home and abroad. In order to make up for this gap, it is necessary to prepare benzophenone artificial antigen

Method used

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  • Preparation method of artificial antigen of 4-aminobenzophenone
  • Preparation method of artificial antigen of 4-aminobenzophenone

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Embodiment Construction

[0019] (1) Preparation of artificial antigen

[0020] Dissolve 0.1mmol of 4-aminobenzophenone in 6mL of N,N-dimethylformamide, ice-bath, add 1mL of 2mol / L HCl, add 13mL of water, ice-bath, add dropwise 1% NaNO 2 solution (excess), stirring continuously in an ice bath. NaNO 2 The amount of solution added can be monitored with starch-iodide test paper, and after the test paper turns blue-black, continue to react for 15 minutes. For liquid A.

[0021] Bovine serum albumin BSA 0.002mmol dissolved in pH 9.6, 0.05mol / L carbonate buffer solution is B solution.

[0022] Add liquid A dropwise to liquid B, adjust the pH to 9.0 while adding, incubate at 4°C for half an hour, and react at room temperature for 4 hours to obtain the artificial antigen mixture.

[0023] Dialysis bag pre-treatment: Take a 10cm dialysis bag, boil it in boiling water for 5 minutes, rinse it with 60°C deionized water for 3 minutes, and store it in 4°C deionized water for later use.

[0024] Transfer the art...

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Abstract

The invention relates to a preparation method of an artificial antigen of 4-aminobenzophenone, belonging to the technical field of biochemical industry. The 4-aminobenzophenone is adopted as a half antigen because benzophenone has no radical necessary for coupling but has the same matrix as the 4-aminobenzophenone. In the invention, the artificial antigen of 4-aminobenzophenone is prepared by utilizing an analog of benzophenone, the 4-aminobenzophenone is a half antigen and is coupled with a carrier BSA (Bovine Serum Albumin) by a diazotization method, and the coupling ratio of the coupling substances is measured by a spectrophotometry. The artificial antigen of 4-aminobenzophenone is successfully synthesized by using the invention, and the synthesis steps are simple and effective. The invention can be fully applied to immunoassay, provide necessary artificial antigens for later research of people and satisfy the national needs for researching the benzophenone and the 4-aminobenzophenone.

Description

technical field [0001] The invention discloses a method for preparing a general artificial antigen of 4-aminobenzophenone, which belongs to the technical field of biochemical industry. Background technique [0002] Benzophenone is mainly used in plastics as a light stabilizer, which can effectively protect food plastic packaging and plexiglass from deterioration due to light, and is also an intermediate of ultraviolet absorbers, organic pigments, medicines, spices, and pesticides. Due to their volatility, benzophenones can migrate from food packaging into food without functional hindrance. Studies have found that benzophenone can seriously damage the liver and kidney tissues of animals, and cause chronic cancer and reproductive toxicity. The EU Food Safety Authority has formulated the maximum migration limit of printing ink food packaging containing benzophenone and 4-aminobenzophenone, and stipulates that the migration limit value of benzophenone in printing ink materials ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765G01N21/31
Inventor 胥传来许宙林菲宋姗姗陈连君
Owner JIANGNAN UNIV
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