Hybridoma cell strain capable of secreting anti-dinitolmide monoclonal antibody and application of hybridoma cell strain

Abstract

The invention relates to a hybridoma cell strain capable of secreting anti-dinitolmide monoclonal antibody and an application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The hybridoma cell strain DAS3H10 capable of secreting the anti-dinitolmide monoclonal antibody is preserved in China General Microbiological Culture Collection Center (CGMCC), the address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the strain is classified and named as a monoclonal cell strain, the preservation date is November 28, 2019, and the preservation number is CGMCC No. 19165. A monoclonal antibody prepared from the hybridoma cell strain DAS3H10 has relatively high affinity and high sensitivity todinitolmide, has 50% inhibitory concentration IC50 on dinitolmide of being 9.01 ng / mL, and can be used for preparing an immunodetection test kit and a colloidal gold test strip of dinitolmide. A powerful detection method and means are provided for detection of dinitolmide in animal-derived food.

Description

technical field [0001] The invention relates to a hybridoma cell strain secreting anti-dinitramine monoclonal antibody and application thereof, which belongs to the field of food safety immune detection. Background technique [0002] Dinitramide (DTM) is a poultry feed additive widely used as a nitroamide anticoccidiostat for its effectiveness in the treatment and prevention of coccidiosis. Stability, low toxicity, less drug resistance and low cost are also reasons for its widespread use in animal feed. However, excessive use and long-acting drugs will lead to residues of veterinary drugs in poultry tissues, which will endanger people's health. In addition, excessive use will also cause most of the veterinary drugs ingested by poultry to be excreted in the form of metabolites or prototype drugs, which is harmful to the environment. constitute a potential threat. Therefore, the Chinese Ministry of Agriculture and the U.S. Food and Drug Administration have formulated strict ...

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Problems solved by technology

These methods have some drawbacks to varying degrees: time-consuming, expensive instruments and extensive sample pretreatment procedures, etc.

But the high sensitivity and reliability of nanotechnology, which is highly dependent on precise data acquisition, requires analytical systems, which means that the application of these methods in field analysis is limited.

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