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Synthetic method of carbostyrile kind antibiotic multi cluster antigen

A technology of multi-cluster antigens and quinolones, which is applied in the field of biochemical industry to achieve the effect of simple synthesis steps

Inactive Publication Date: 2008-04-09
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In order to establish a rapid detection method, it is necessary to synthesize a multi-cluster antigen of quinolone antibiotics. So far, no domestic reports have been found on the synthesis method of multi-cluster antigens of quinolone antibiotics.

Method used

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  • Synthetic method of carbostyrile kind antibiotic multi cluster antigen
  • Synthetic method of carbostyrile kind antibiotic multi cluster antigen
  • Synthetic method of carbostyrile kind antibiotic multi cluster antigen

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Experimental program
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Embodiment Construction

[0030] (1) Synthesis of artificial hapten

[0031] Synthesis:

[0032] Synthesis of product 1:

[0033] In a 1L round bottom flask, add 500mL of methanol, 2-bromoethylamine hydrobromide (10g, 49mmol), triethylamine (30mL), di-tert-butyl carbonate Boc 2 O (13g, 60mmol), stirred, heated to 60°C, reacted for 1h, then reacted overnight at room temperature. The reaction solution was concentrated in vacuo, the residue was dissolved in 500mL of dichloromethane, washed with 0.5N hydrochloric acid (250mL×2), saturated saline (250mL), saturated NaHCO 3 (250 mL), dried over anhydrous magnesium sulfate, filtered, and concentrated to give product 1 as a light yellow liquid (yield 63%), which was directly used in the next reaction.

[0034] Synthesis of product 2:

[0035] In a 250ml round bottom flask, add norfloxacin (5.499g, 17.2mmol), DMF (70mL), stir well, add triethylamine (4.8mL, 35mmol), then add product 1 (4.631g, 20.6mmol ), heated up to 80°C, and reacted overnight. Cool the...

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Abstract

The invention relates to a synthetic method of quinolones antibiotic antigen, which belongs to the biological chemistry technical field. The raw material of the invention is the norfloxacin, the hapten N-2-ethylamine-norfloxacin is produced through the derivatization of norfloxacin and the protection of carboxyl, and the quinolones antibiotic multi-cluster antigen N-2-ethylamine-norfloxacin-bovine serum albumin is compounded by the coupling of the albumin and the hapten N-2-ethylamine-norfloxacin. The invention successfully compounds the quinolones antibiotic multi-cluster antigen, and the combining procedure is simple; and the purity quotient of the quinolones antibiotic antigen can reach more than 99 percent after the purification. The invention can be used for the application of quinolones multi-cluster EIA kits, and the invention provides a convenient way for a further research, and the need of the research of the invention at home can be satisfied.

Description

technical field [0001] The invention relates to a method for synthesizing multi-cluster antigens of quinolone antibiotics, belonging to the technical field of biochemical engineering. Background technique [0002] Quinolones are a class of synthetic antibiotic drugs, English name: quinolones (QNS). Chemically related to nalidixic acid, its mechanism of action is to directly act on the nucleus of bacteria, inhibit the DNA gyrase of bacteria, so that the enzyme cannot introduce nicks on the double strand of DNA, destroy the metabolism and reproduction of bacteria, and quickly kill bacteria . Originally used to treat urinary tract infections, these drugs have now been developed to treat systemic infectious diseases, and are commonly used as preventive and therapeutic drugs in animal breeding. In recent years, the residues of these drugs in animal tissues have attracted widespread attention. my country has stipulated that danofloxacin, difloxacin, difloxacin, The maximum resid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53C07K14/765C07K1/10C07D215/233
Inventor 胥传来李雅丽王灿辉唐剑峰陈伟刘丽强陶冠军秦昉
Owner JIANGNAN UNIV
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