Hybridoma cell strain capable of secreting anti-pendimethalin monoclonal antibody and application of hybridoma cell strain

Abstract

The invention discloses a hybridoma cell strain capable of secreting an anti-pendimethalin monoclonal antibody and application of the hybridoma cell strain, and belongs to the field of food safety immunodetection. The invention provides the hybridoma cell strain capable of secreting the anti-pendimethalin monoclonal antibody, wherein the hybridoma cell strain is classified and named as a monoclonal cell strain, the preservation date is September 27, 2020, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.20791. The pendimethalin complete antigen and the same amount of Freund's adjuvant are mixed and emulsified, and BALB / c mice are immunized through neck and back subcutaneous multipoint injection. The hybridoma cell strain is prepared by the following steps of fusing splenocytes of high-titer and low-IC50 mice with mouse myeloma cells through a PEG (Polyethylene Glycol) method, and screening out hybrid cells after fusing the two cells by adopting a selective culture medium; and screening cells by an indirect competitive enzyme-linked immunosorbent assay and subcloning for three times to finally obtain the monoclonal antibody hybridoma cell strain ACE. The monoclonal antibody secreted by the hybridoma cell strain ACE has relatively high affinity and sensitivity to the pendimethalin, and a powerful detection method and means are provided for detection of the pendimethalin in animal-derived food.

Description

technical field [0001] The invention relates to a hybridoma cell strain secreting anti-pendimethalin monoclonal antibody and an application thereof, belonging to the field of food safety immune detection. Background technique [0002] Pendimethalin is a dinitroaniline herbicide, the chemical name is N-(1-ethylpropyl)-2,6-dinitro-3,4-dimethylaniline, also known as pendimethalin Ling, Shi Tianbu, Chuyatong, etc. Pendimethalin is a high-efficiency, low-toxic herbicide. The mechanism of action is that pendimethalin enters the plant body and combines with tubulin to inhibit the division and elongation of plant cells. In recent years, it has been widely used in the weeding of corn, potato, rice, soybean, tobacco, vegetables and other crops. Its widespread use has resulted in detection in soil, groundwater, surface water, and air, causing significant harm to ecosystems and human health. [0003] There have been a few reports at home and abroad on the detection methods of pendime...

AI Technical Summary

Problems solved by technology

Instrumental detection methods can perform quantitative analysis and have a low detection limit, but usually require expensive instruments and complicated operations, and the pretreatment and detection time is long, which seriously restricts the promotion of these detection methods

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