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Method for preparing diazepam artificial antigen

A technology of artificial antigen and diazepam, applied in animal/human protein, serum albumin, instruments, etc., can solve the problems that cannot meet the requirements of fast, convenient and accurate detection, and achieve the effect of simple synthesis steps

Active Publication Date: 2008-04-16
山东美正生物科技有限公司
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  • Abstract
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Problems solved by technology

At present, the detection of diazepam at home and abroad mainly includes high-performance liquid chromatography (HPLC), gas chromatography (GC), thin-layer chromatography (TLC), gas-mass spectrometry (GC-MS), liquid-mass However, these methods not only require expensive instruments and equipment, but also have relatively high requirements for test materials, which require further purification treatment, which can no longer meet the requirements of modern detection for fast, convenient and accurate
In recent years, foreign countries have carried out research on benzodiazepine immunoassay methods, but there is still a blank in this area in China

Method used

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  • Method for preparing diazepam artificial antigen
  • Method for preparing diazepam artificial antigen
  • Method for preparing diazepam artificial antigen

Examples

Experimental program
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Embodiment Construction

[0024] (1) Preparation of artificial hapten:

[0025] Weigh 2.5mmol of anhydrous aluminum chloride and 1mmol of succinic anhydride into a 50mL round-bottomed flask, add 10mL of nitrobenzene, and stir at 40°C until the solids are completely dissolved. Another 1 mmol of diazepam was weighed and added into the above reaction solution in 3 times, the reaction temperature was gradually increased to 70° C., and the reaction was carried out overnight. Under vigorous stirring, the reaction solution was poured into a certain amount of ice water, and after standing for stratification, the organic layer was taken for vacuum distillation, and the obtained residue was dissolved with a small amount of hot methanol solution, and precipitated at low temperature, filtered and dried to obtain 0.8 mmol half Antigen back up.

[0026] Reaction end point monitoring: for the production of silica gel thin-layer plates, weigh 12g of silica gel, dissolve it in 40mL of 0.5% mass concentration sodium hy...

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Abstract

A preparation method of an artificial antigen of diazepam is disclosed, which belongs to the biochemistry technology field. The first step of the present invention is preparation and detection of a semi-antigen, in which diazepam and succinic anhydride are used as the raw materials, and a carboxyl is inducted to the 5' benzene ring through Friedel-Crafts acylation, the second step is the preparation and detection of an artificial antigen, in which the product of step one is combined with bovine serum protein through mixed acid anhydride method to prepare the artificial antigen of diazepam, i.e. the diazepam-bovine serum protein. The present invention synthesizes the artificial antigen of diazepam, with simplified and effective synthetic procedures, as well as feasibility to be applied to immune analysis, thereby providing a convenient path for the further research by the people, and being capable of satisfying the domestic needs on the study on the object.

Description

technical field [0001] The invention discloses a method for preparing an artificial antigen of diazepam, which belongs to the technical field of biochemistry. Background technique [0002] Diazepam is a benzodiazepine sedative and hypnotics, chemical name: 1-methyl-5-phenyl-7-chloro-1,3-dihydro-2H-1,4-benzodiazepine Zol-2-one. It has been used as an animal feed additive, as a growth promoter, which can make animals sleepy and less active, grow faster and change the meat quality. It is also used for sedation before slaughtering and animal quarantine, or before transferring animals to slaughter, so as to reduce animal tension and reduce animal injury and mortality. Drowsiness, weakness, headache, dizziness, nausea, constipation, and occasional rash, liver damage, bone marrow suppression and other adverse reactions can be seen with these drugs. If such drugs are randomly added to the feed, the accumulated drugs will enter the human body through the food chain, which will cau...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765G01N33/531G01N33/536C07D243/38
Inventor 胥传来李秋生陶冠军秦昉徐一平袁媛胡拥民陈伟彭池方
Owner 山东美正生物科技有限公司
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