Synthetic method of artificial antigen suitable for alkylphenol medicament

A technology of artificial antigen and synthesis method, which is applied to the preparation method of peptides, chemical instruments and methods, animal/human proteins, etc., to achieve the effect of simple synthesis steps

Inactive Publication Date: 2012-01-18
WUXI ENTRY EXIT INSPECTION & QUARANTINE BUREAU PEOPLES REPUBLIC OF CHINA +1
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AI Technical Summary

Problems solved by technology

So far, there is no report on the immunological detection method for the residues of alkylphenol structure at home and abroad. For this purpose, a hapten for alkylphenols was designed and synthesized, using the degradation product of alkylphenol, nonylphenol analogue NPA, as the hapten. Complete antigen for structure detection

Method used

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  • Synthetic method of artificial antigen suitable for alkylphenol medicament
  • Synthetic method of artificial antigen suitable for alkylphenol medicament
  • Synthetic method of artificial antigen suitable for alkylphenol medicament

Examples

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Embodiment 1

[0020] Preparation of nonylphenol analogues: get 100mmol of dimethyl pimelate and 100mmol of pimelic acid, hydrolyze the reaction for 12 hours in a sodium hydroxide alkaline solution with a pH of 11 to obtain pimelic acid ester, and then add A sufficient amount of thionyl chloride is reacted in benzene to convert the chloropimelic acid ester into chloropimelic acid ester; the chloropimelic acid ester is dropped into the dichloroethane solution of anisole, at 0 Stir the reaction at ℃ for 24 hours, extract with ether, and then use zinc powder and mercuric acetate to reflux in toluene for 6 hours; spin evaporate to dryness, dissolve with ethanol, and add 10% sodium hydroxide solution to reflux for 6 hours, and then The precipitate was obtained by rotary evaporating to dryness; the precipitate was dissolved with glacial acetic acid, and 48% hydrogen bromide was added to reflux for reaction for 6 hours, then evaporating to dryness, and recrystallized in ethanol to obtain the carboxy...

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Abstract

The invention relates to a synthetic method of an artificial antigen suitable for alkylphenol medicaments. According to the technical scheme provided by the invention, nonyl phenol analogues are mainly used as haptens; the analogues are coupled with carrier protein bovine serum albumin (BSA) by an activated ester method; and the coupling ratio of the conjugate is determined by polyacrylamide gel electrophoresis. The synthetic method of the present invention can successfully synthesize an artificial antigen of nonyl phenol analogues; the synthetic steps are simple and effective, and can be used in immunoassays completely; the invention provides a convenient approach for future research, and can meet the requirements for domestic research.

Description

technical field [0001] The invention belongs to the field of biochemical technology, and in particular relates to a synthesis method suitable for artificial antigens of alkylphenol drugs. Background technique [0002] Alkylphenol compounds (Alkyl Phenol, APs) are an important kind of endocrine disrupting substances, which have obvious environmental estrogenic effects. Most of these compounds are fat-soluble organic compounds with strong chemical stability. Once ingested, they are not easily degraded and excreted. They are bioaccumulative and highly harmful. Even a small amount can affect organisms. At the same time, it is widely distributed in the environment and its content is low. Alkylphenol compounds APs are important fine chemical raw materials, used in surfactants, lubricating oil additives, oil-soluble phenolic resins and insulating materials, textile printing and dyeing, paper-making auxiliaries, pesticide emulsifiers, industrial detergents, anti-aging of rubber and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K1/113
Inventor 殷祥刚胥传来董激文匡华周亮季晓丹金勉勉
Owner WUXI ENTRY EXIT INSPECTION & QUARANTINE BUREAU PEOPLES REPUBLIC OF CHINA
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