General use artificial antigen synthesis of aminoglycoside medicaments

Abstract

The invention relates to a method for synthesizing general artificial antigen of aminoglycoside medicine, belonging to the biochemical engineering technical field. The method uses neamine as hapten, uses a glutaraldehyde method to couple the neamine with protein BSA as a carrier, and uses a gel electrophoresis method to measure the coupling ratio of coupled matter. The method successfully synthesizes the artificial antigen of neamine, is simple and effective in synthesis method, can be used in immune analysis completely, provides the necessary artificial antigen for after research of people, and can meet the needs of the research on the aminoglycoside medicine in China.

Description

A kind of synthetic method of general artificial antigen of aminoglycoside drugs technical field The invention discloses a method for synthesizing a general artificial antigen of aminoglycoside drugs, which belongs to the technical field of biochemical engineering. Background technique Neomycin, English name: Neamin, CAS No. 5144-52-5, molecular formula C12H25N4O6, commonly known as neomycin A, is a structural community of aminoglycoside drugs. Aminoglycosides are a class of antibiotics that combine amino sugar molecules with non-sugar glycogens. These antibiotics are widely used and are broad-spectrum antibiotics for therapeutic drug monitoring. Its function is characterized by the ability to bind to the ribosomal 30S subunit, causing it to synthesize abnormal proteins, hindering the release of easily synthesized proteins, thereby inhibiting the growth of bacteria. Clinically, aminoglycoside antibiotics are mainly used to treat intestinal infections caused by Escherichi...

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Problems solved by technology

However, due to its improper use or non-compliance with the withdrawal period regulations, etc., it can cause its residues in animal products, which will bring serious harm to human health, such as destroying the balance of gastrointestinal flora and enhancing bacterial resistance, etc.

Existing microbial detection methods and instrumental detection methods are difficult to achieve rapid, accurate and multi-residue detection of aminoglycoside antibiotics

At present, there are no reports on ideal multi-residue immunoassay methods for aminoglycoside drugs at home and abroad

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