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Antibacterial nanomaterial, and preparation method and application thereof

A nanomaterial and gold nanoparticle technology, applied in the field of aminophenol-modified gold nanoparticles and their preparation, can solve the problems of unclear antibacterial target and mechanism, difficult circulation and storage in the body, difficult circulation and metabolism, etc. Excellent biocompatibility, clear antibacterial target, simple and fast preparation method

Active Publication Date: 2020-09-01
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the obtained poly-ortho-aminophenol antibacterial material is micron-sized, and it is not easy to circulate and metabolize in the body. At the same time, its preparation process requires the use of organic solvents such as strong acids. If it remains in the body, it will cause biological toxicity and environmental pollution.
[0008] In view of the shortcomings of existing antibacterial materials such as easy drug resistance, poor biological safety, unclear antibacterial targets and mechanisms, complicated preparation methods, large size, which is not conducive to internal circulation and difficult storage, there is an urgent need to develop a nanoscale, Antibacterial nanomaterials with excellent biocompatibility and clear antibacterial mechanism to deal with infections caused by multi-drug resistant bacteria

Method used

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  • Antibacterial nanomaterial, and preparation method and application thereof
  • Antibacterial nanomaterial, and preparation method and application thereof
  • Antibacterial nanomaterial, and preparation method and application thereof

Examples

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Embodiment 1

[0073] The present embodiment provides an antibacterial nanomaterial, the antibacterial nanomaterial is gold nanoparticles (2AP_Au NPs) modified by o-aminophenol, and its specific preparation steps are as follows:

[0074] (1) In a round bottom flask, 0.05 mmol of chloroauric acid trihydrate and 0.05 mmol of o-aminophenol (2AP, molecular weight 109.13) were added to 10 mL of deionized water. Then 50 μL of triethylamine and 30 mg of Tween 80 were added, and mixed in an ice-water bath for 10 min until the molecules were completely dissolved.

[0075] (2) Dissolve 6 mg of sodium borohydride in 2 mL of deionized water, and add it dropwise to a round bottom flask under vigorous stirring at 1000 r / min. The color of the solution in the bottle immediately changes from colorless to brown. Keep this reaction condition for another reaction 2h.

[0076] (3) The obtained gold nanoparticles reduced by anthranilic acid were dialyzed for 24 h with a dialysis bag to remove untreated chemical ...

Embodiment 2

[0079] The present embodiment provides an antibacterial nanomaterial, the antibacterial nanomaterial is gold nanoparticles (3AP_Au NPs) modified by m-aminophenol, and its specific preparation steps are as follows:

[0080] (1) In a round bottom flask, add 0.05 mmol of chloroauric acid trihydrate and 0.05 mmol of m-aminophenol (3AP, molecular weight 109.13) into 10 mL of deionized water. Then 50 μL of triethylamine and 30 mg of Tween 80 were added, and mixed in an ice-water bath for 10 min until the molecules were completely dissolved.

[0081] Steps (2) and (3) are the same as the steps for preparing o-aminophenol-modified gold nanoparticles in Example 1.

[0082] The morphology of 3AP_Au NPs was characterized by transmission electron microscopy, and the observation results are shown in Figure 3(a) and Figure 3(b). Among them, Fig. 3 (a) is the morphological characterization diagram of gold nanoparticles when the scale is 50nm, and Fig. 3 (b) is the morphological characteriza...

Embodiment 3

[0084] The present embodiment provides an antibacterial nanomaterial, the antibacterial nanomaterial is gold nanoparticles (4AP_Au NPs) modified by p-aminophenol, and its specific preparation steps are as follows:

[0085] (1) In a round bottom flask, add 0.05 mmol of chloroauric acid trihydrate and 0.05 mmol of p-aminophenol (4AP, molecular weight 109.13) into 10 mL of deionized water. Then 50 μL of triethylamine and 30 mg of Tween 80 were added, and mixed in an ice-water bath for 10 min until the molecules were completely dissolved.

[0086] Steps (2) and (3) are the same as the steps for preparing o-aminophenol-modified gold nanoparticles in Example 1.

[0087] The morphology of 4AP_Au NPs was characterized by transmission electron microscopy, and the observation results are shown in Figure 4(a) and Figure 4(b). Wherein, Fig. 4(a) is a morphological characterization diagram of gold nanoparticles when the scale is 50nm, and Fig. 4(b) is a morphological characterization diag...

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Abstract

The invention provides an antibacterial nanomaterial, and a preparation method and application thereof. The antibacterial nanomaterial is aminophenol modified gold nanoparticles, and aminophenol is modified on the surfaces of the gold nanoparticles through gold-ammonia bonds. The aminophenol is combined with the gold nanoparticles, then hydroxyl groups on the surface of the obtained antibacterialnanomaterial can target polysaccharides on the surface of bacteria, permeability of cell membranes is improved by destroying cell walls of the bacteria, aminophenol serves as a functional group to becombined with 16S ribosome RNA of the bacteria, protein synthesis is inhibited, then the bacteria are killed, and a good antibacterial effect is achieved. Meanwhile, the surface of the antibacterial nanomaterial is negatively charged, so that the antibacterial nanomaterial has excellent biocompatibility, does not induce bacteria to generate drug resistance, and has higher biological safety compared with renal toxicity drugs such as aminoglycoside drugs and the like.

Description

technical field [0001] The invention belongs to the field of biological materials, and in particular relates to an antibacterial nanometer material and its preparation method and application, in particular to an aminophenol-modified gold nanoparticle with antibacterial effect and its preparation method and application. Background technique [0002] Increased antibiotic resistance is a major public health concern, and the prevalence of multidrug-resistant strains has become a serious problem, hindering the treatment of serious infectious diseases worldwide. It is known that more than 70% of bacteria are resistant to one or more antibiotics. Gram-negative bacteria (such as E. coli) can cause infections of the urinary tract, gastrointestinal tract, and lungs, while gram-positive bacteria (such as Staphylococcus aureus) can cause infections of the skin and cartilage tissues. [0003] Aminoglycoside antibiotics are a class of broad-spectrum antibiotics that have been widely used...

Claims

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Application Information

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IPC IPC(8): A01N59/16A01N33/10A01P1/00A61K31/136A61K33/242A61K47/55A61P31/04
CPCA01N33/10A01N59/16A61K31/136A61K47/55A61P31/04A61K33/242A61K2300/00
Inventor 蒋兴宇王乐
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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