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Veterinary drug multi-residue immune detection system and detection method based on smart phone

A multi-residue, veterinary drug technology, applied in measurement devices, material analysis, instruments and other directions by observing the impact on chemical indicators, can solve problems such as inability to meet on-site detection, acquisition and signal processing dependencies, and achieve immediate display of test results. , low cost, the effect of image acquisition capability

Inactive Publication Date: 2018-09-11
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no immune chip for various veterinary drug residues
[0004] In addition, the immune chip realizes the simultaneous detection of multiple substances by forming an antigen array and applying a marker, but image acquisition and signal processing rely on scanners and computers, which still cannot meet the requirements of on-site detection.

Method used

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  • Veterinary drug multi-residue immune detection system and detection method based on smart phone
  • Veterinary drug multi-residue immune detection system and detection method based on smart phone
  • Veterinary drug multi-residue immune detection system and detection method based on smart phone

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Embodiment 1

[0041] A smart phone-based veterinary drug multi-residue immune detection system, the construction method of the detection system is carried out according to the following steps:

[0042] (1) Determine the optimal working concentration of the four veterinary drug residue antigens

[0043] Set different concentration gradients for the working concentrations of the antigens of the four veterinary drug residues, and set up three controls, according to the chip design diagram ( figure 1 ) onto the chip in turn to prepare an immune chip, which competes with the antibodies of the four veterinary drug residues for immunoreaction to obtain the optimal working concentration of the four veterinary drug residual antigens and the optimal working concentration of the antibody, which are recorded by the scanner As a result, the gray value of the chromogenic image was read with Adobe Photoshop CC2015. The result is as figure 2 As shown, ractopamine, β 2 - The optimal working concentratio...

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PUM

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Abstract

The invention discloses a veterinary drug multi-residue (ractopamine, beta2-stimulant, chloramphenicol, florfenicol and metabolin florfenicol amine) immune detection system which comprises an immune chip for detecting veterinary drug multi residues, an image collecting device and mobile equipment provided with a detection APP, wherein the detection APP can collect an immune chip color developing image and read an image gray value, and operation of the detection APP to collect the immune chip color developing image is finished in the image collecting device; a standard curve between the immunechip color developing image gray value and a veterinary drug residue standard content is set in the detection APP. In detection, the immune chip is utilized to detect a target article to be detected;after immune chip color developing is stable, the image collecting device is utilized, and the detection APP is utilized to collect an immune chip color developing image reading gray valve; thus, a content of the article to be detected is analyzed out, a detection result is directly displayed on a screen of the mobile equipment, and the purpose of quickly detecting varieties of veterinary drug residues in site is achieved.

Description

technical field [0001] The invention relates to the technical field of food safety, more specifically, to a smart phone-based veterinary drug multi-residue immunological detection system and detection method. Background technique [0002] Veterinary drug residues are an important factor affecting food safety, and their residues in animal-derived foods seriously endanger the physical and mental health of consumers, which has aroused widespread concern. According to the definition of the United Nations Food and Agriculture Organization (FAO) and the World Health Organization (WHO) Joint Legislative Committee on Veterinary Drug Residues, veterinary drug residues refer to the original drugs that accumulate or remain in livestock and poultry bodies or products (such as eggs, milk, meat, etc.) or its metabolites, including residues of impurities associated with veterinary drugs. After animals use drugs, the drugs enter the ecological environment in the form of prototypes or metab...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/82
CPCG01N21/82G01N33/54306
Inventor 杨金易范丛丛孙远明梁早清王弘雷洪涛沈玉栋徐振林张东升许小炫
Owner SOUTH CHINA AGRI UNIV
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