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Method for detecting avermectins pesticide multi-residues in cereal agricultural products

A technology with many residues of abamectins and pesticides, applied in measuring devices, instruments, fluorescence/phosphorescence, etc., can solve the problem of very little research on abamectins pesticide residues

Inactive Publication Date: 2010-11-24
上海市农业技术推广服务中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since abamectin pesticides have only been approved for use in rice in recent years, most of the previous literature reports are about the analysis methods of abamectin pesticide residues in livestock products and fruit and vegetable agricultural products. There are very few studies on the analysis methods of mycocin pesticide residues
Through patent search, it is found that there is currently no patent on the multi-residue analysis method of abamectin pesticides in cereal agricultural products

Method used

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  • Method for detecting avermectins pesticide multi-residues in cereal agricultural products
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  • Method for detecting avermectins pesticide multi-residues in cereal agricultural products

Examples

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Embodiment 1

[0038]Example 1: Detection of multiple residues of abamectin pesticides in rice

[0039] The first step: sample pretreatment:

[0040] After the rice sample is crushed with a grinder, pass through a 100-mesh sieve (the sample cannot be measured immediately and stored in a -20°C refrigerator). Weigh 20 g of the crushed sample and place it in a 100 mL flat-bottomed glass bottle, add 50 mL of acetonitrile, and the sample is heated at 14000 r / min After homogenizing with a high-speed homogenizer for 2 minutes, place it in a microwave-assisted extraction device for 20 minutes;

[0041] After standing still for 1 hour, filter the extracted sample filter paper into a 100mL stoppered measuring cylinder filled with about 5g of sodium chloride, shake the filtrate vigorously for 5 minutes and then let it stand for 30 minutes, and use the salting-out effect to separate the organic phase;

[0042] Draw 10 mL of the extract from the organic layer and transfer it to a glass test tube, then d...

Embodiment 2

[0061] Example 2: Detection of Abamectin Pesticide Residues in Wheat

[0062] The first step: sample pretreatment:

[0063] After the wheat sample is pulverized with a grinder, pass through a 100-mesh sieve (the sample cannot be measured immediately and stored in a -20°C refrigerator), weigh 30 g of the pulverized sample and place it in a 100 mL flat-bottomed glass bottle, add 90 mL of acetonitrile, and the sample is heated at 14000 r / After homogenizing for 2 minutes with a high-speed homogenizer, place it in a microwave-assisted extraction device for 20 minutes;

[0064] After standing still for 1 hour, filter the extracted sample filter paper into a 100mL stoppered measuring cylinder filled with about 5g of sodium chloride, shake the filtrate vigorously for 5 minutes and then let it stand for 30 minutes, and use the salting-out effect to separate the organic phase;

[0065] Draw 20 mL of the extract from the organic layer and transfer it to a glass test tube, then dry it w...

Embodiment 3

[0084] Example 3: Detection of multiple residues of abamectin pesticides in corn

[0085] The first step: sample pretreatment:

[0086] After the corn sample is pulverized by a grinder, pass through a 100-mesh sieve (if the sample cannot be measured immediately, store it in a -20°C refrigerator), weigh 10 g of the crushed sample and place it in a 100 mL flat-bottomed glass bottle, add 30 mL of acetonitrile, and the sample is stored at 14000 r / After homogenizing for 2 minutes with a high-speed homogenizer, place it in a microwave-assisted extraction device for 15-20 minutes;

[0087] After standing still for 1 hour, filter the extracted sample filter paper into a 100mL stoppered measuring cylinder filled with about 5g of sodium chloride, shake the filtrate vigorously for 5 minutes and then let it stand for 30 minutes, and use the salting-out effect to separate the organic phase;

[0088] Draw 10 mL of the extract from the organic layer and transfer it to a glass test tube, th...

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Abstract

The invention discloses a method for detecting avermectins pesticide multi-residues in cereal agricultural products, which comprises the following steps of: extracting the avermectins pesticide residues from the cereal agricultural products with acetonitrile, separating an organic phase containing avermectins pesticides from a water phase under the action of salting-out, performing fluorescence derivatization on the extracted avermectins pesticides by a trifluoro acetic anhydride (TFAA)-N-methylimidazole (NMIM)-acetonitrile (ACN) method in an anhydrous state, and realizing the trace residue analysis of the pesticides by adopting a high performance liquid chromatography-fluorescence detection method. The method of the invention has the advantages of performing trace analysis and detection on the residues of avermectin, emamectin benzoate and ivermectin in a plurality of cereal agricultural products, such as rice, wheat, corn and the like, along with simplicity, convenience, time saving, low cost and the like.

Description

technical field [0001] The invention relates to a method for detecting and analyzing pesticide residues in agricultural products, in particular to a method for detecting multiple residues of avermectin pesticides in cereal agricultural products. Background technique [0002] Avermectin (AVM for short) is a sixteen-membered macrolide diglycoside compound isolated from the fermentation product of Streptomyces avermitilis in 1976 by Satoshi Omura of Kitasato University in Japan and Merck of the United States. . This type of compound has 4 main components: A1a, A2a, B1a, B2a; 4 minor components: A1b, A2b, B1b, B2b, among which B1a has the strongest anti-insect activity. Abamectin is Avermectin B1, containing Avermectin B1a≥80%, Avermectin B1b≤20%. After Abamectin was put into the market as a pesticide in 1985, a series of new derivatives with higher activity, stronger selectivity and better safety have been developed with Abamectin as the parent body through semi-synthesis and...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N30/02
Inventor 赵莉谢显传杨挺占绣萍
Owner 上海市农业技术推广服务中心
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