ELISA detection method for carbostyril antibiotic relict

An enzyme-linked immunosorbent assay, quinolone technology, applied in measurement devices, color/spectral property measurement, instruments, etc., can solve the problems of high price, unannounced popularity, and complicated monoclonal antibody preparation process.

Inactive Publication Date: 2008-12-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above method requires the use of monoclonal antibodies, and the preparation process of monoclonal antibodies is complicated, expensive, and unpopular.
At present, there is still a lack of practical methods for the detection of multiple residues of quinolone antibiotics in animal foods

Method used

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  • ELISA detection method for carbostyril antibiotic relict
  • ELISA detection method for carbostyril antibiotic relict
  • ELISA detection method for carbostyril antibiotic relict

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0043] specific implementation plan

[0044] The present invention is further illustrated by the following examples.

[0045] 1. Instrument:

[0046] TGL-40B desktop low-speed centrifuge Shanghai Anting Scientific Instrument Factory;

[0047] KFLO pure water machine Kevron company;

[0048] ZD-9556 horizontal shaking table Taicang Science and Education Equipment Factory;

[0049] Costar 96-well 8×12 detachable enzyme plate Shanghai Jitai Biotechnology Co., Ltd.;

[0050] MuLtiska Mks microplate reader Thermo Labsystems;

[0051] Adjustable pipette Thermo Labsystems, homogenizer Fluka;

[0052] Vortex mixer Shanghai Huxi Instrument Analysis.

[0053] 2. Reagents:

[0054] Horseradish peroxidase-labeled goat anti-rabbit IgG (HRP-IgG) Kangcheng Bioengineering Company;

[0055] Tetramethylbenzidine (TMB) Huamei Bioengineering Company;

[0056] All other reagents were of analytical grade.

[0057] 3. Steps

[0058] 1. Synthesis of Immunogen and Coatogen

[0059] Synthet...

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PUM

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Abstract

The invention relates to a method for quinoxalone antibiotics multi-residue enzyme-linked immune detection, and belongs to the technology field of the antibiotics residue detection method. In the method, the polyclonal antibodies are obtained by utilizing syntactic quinoxalone antibiotics multi-cluster antigen immunization; the coupling compound of the half antigen of the quinoxalone antibiotics and an egg-white protein OVA is taken as an envelope antigen; the quinoxalone antibiotics is taken as a standard; the indirect competitive enzyme-linked immune detection method for the quinoxalone antibiotics in animal food is established; a rapid and high efficient detection means is provided for the residues of the quinoxalone antibiotics in the animal food; because the polyclonal antibodies are adopted, the cost is low, and the stability and the repeatability are good; the detection limit (IC is smaller than 90) is 0.0126 ng / ml, the median inhibitory dose (IC is smaller than 50) is 0.52 ng / ml, and the detection range (IC is smaller than 20 to 80) is 0.04467 to 14 ng / ml. In addition, the ELISA method is also evaluated in the aspects of specificity, accuracy and sensitivity, thereby conforming to the detection requirements for the multi-residue ELISA method.

Description

technical field [0001] The multi-residue enzyme-linked immunosorbent assay method for quinolone antibiotics, specifically, uses enzyme-linked immunosorbent assay (ELISA) to detect the residues of quinolone antibiotics in animal foods. The invention belongs to the technical field of antibiotic residue detection method. Background technique [0002] Quinolones are a class of synthetic antibiotic drugs, which are chemically related to nalidixic acid. Their mechanism of action is to directly act on the nucleus of bacteria and inhibit the DNA gyrase of bacteria, so that the enzyme cannot introduce nicks on the double strand of DNA and destroy bacteria. Metabolism and reproduction, quickly kill bacteria. Originally used to treat urinary tract infections, these drugs have now been developed to treat systemic infectious diseases, and are commonly used as preventive and therapeutic drugs in animal breeding. In recent years, the residues of these drugs in animal tissues have attract...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N21/25
Inventor 胥传来李雅丽
Owner JIANGNAN UNIV
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