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Detecting method of pesticide multi-residue in fresh tea leaves

A technology for the detection of multiple pesticide residues and detection methods, which is applied in the field of detection of multiple pesticide residues in fresh tea leaves. It can solve the problems of lack of detection methods and reports for multiple pesticide residues in fresh tea leaves, and achieve matrix interference avoidance, high sensitivity, and improved detection efficiency. Effect

Inactive Publication Date: 2015-07-08
四川省农业科学院分析测试中心
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the focus of tea quality and safety control is mainly on finished tea, and there is no report on the control method for fresh tea, and there is no report on the detection method of pesticide residues in fresh tea.

Method used

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  • Detecting method of pesticide multi-residue in fresh tea leaves
  • Detecting method of pesticide multi-residue in fresh tea leaves
  • Detecting method of pesticide multi-residue in fresh tea leaves

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Embodiment Construction

[0013] Method of the present invention comprises the following steps:

[0014] 1) Place the homogenized fresh tea sample in a centrifuge tube, add acetonitrile and vortex;

[0015] 2) Vortex after adding anhydrous magnesium sulfate and sodium chloride;

[0016] 3) centrifugal;

[0017] 4) take the centrifuged supernatant for purification;

[0018] 5) Detection by gas chromatography-tandem mass spectrometry.

[0019] The above purification is:

[0020] Clean up with PSA / GCB solid phase extraction column, pre-wash with acetonitrile-toluene; take the supernatant after centrifugation, load the sample; rinse with acetonitrile-toluene; collect the eluate, and concentrate it to near dryness by rotating under reduced pressure; n-hexane + Make up to volume with acetone. Example:

[0021] 1) Weigh 5g of homogenized fresh tea leaves into a 50mL Teflon centrifuge tube, add 20mL of acetonitrile, and vortex for 1 minute;

[0022] 2) Add weighed 4g of anhydrous magnesium sulfate and 1...

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Abstract

The invention provides a detecting method of a pesticide multi-residue in fresh tea leaves. The method comprises the following steps that firstly, a fresh tea leaf sample which is homogenated is placed in a centrifuge tube, and after acetonitrile is added, vortex is carried out; secondly, anhydrous magnesium sulfate and sodium chloride are carried out, and the vortex is carried out; thirdly, centrifuging is carried out; fourthly, centrifuged supernatant liquor is obtained to be subjected to purifying; fifthly, detecting is carried out by adopting the gas chromatography- tandem mass spectrometry method. According to the method, the tea leaves are purified by a PSA / GCB solid phase extraction column, the pesticide residue in the tea leaves are detected by adopting the gas chromatography- tandem mass spectrometry method at the same time, the method is rapid, accurate, high in sensitivity, and suitable for multi-residue screening and detecting in the fresh tea leaves.

Description

technical field [0001] The invention relates to a detection method, in particular to a detection method for multiple residues of pesticides in fresh tea leaves. Background technique [0002] Tea is an important economic crop in my country and one of the most important export products in China. Relevant studies have shown that tea has the functions of anti-cancer and scavenging free radicals in the body, so it is favored by more and more consumers. Tea is a perennial evergreen leafy woody plant belonging to the family Theaceae, generally shrubs or trees. Due to the high requirements on the planting environment and the susceptibility to various pests and diseases during its growth, chemical pesticides are widely used in tea gardens. The detection of pesticide residues mainly includes gas chromatography, gas chromatography-mass spectrometry, and liquid chromatography-mass spectrometry. However, at present, the focus of tea quality and safety control is mainly on finished tea...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 侯雪雷绍荣郭灵安杨晓风韩梅邱世婷刘炜
Owner 四川省农业科学院分析测试中心
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