Broad spectrum specific molecularly imprinted polymer of quinolone medicine, chemiluminescence kit, detection method and application

A chemiluminescence reagent and quinolone technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis through chemical reactions of materials, to achieve strong tolerance, reduce waste of resources, and reduce detection costs

Inactive Publication Date: 2019-01-01
河北英茂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there are no relevant research reports on molecularly imprinted polymer-based chemiluminescence methods or kits for quinolones at home and abroad.

Method used

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  • Broad spectrum specific molecularly imprinted polymer of quinolone medicine, chemiluminescence kit, detection method and application
  • Broad spectrum specific molecularly imprinted polymer of quinolone medicine, chemiluminescence kit, detection method and application
  • Broad spectrum specific molecularly imprinted polymer of quinolone medicine, chemiluminescence kit, detection method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Molecularly imprinted polymer synthesis example 1

[0029] (a) Put 1 part of pseudomolecular template pipemidic acid, 5 parts of functional monomer methacrylic acid, 20 parts of initiator azobisisobutyronitrile and 20 parts of crosslinking agent ethylene glycol dimethacrylate in In chloroform solvent, react at 60°C for 12 hours to obtain solid particles;

[0030] (b) Put the solid particles in a Soxhlet extractor, and continuously reflux the extraction solution of methanol and acetic acid (9:1, V / V) for 12 hours to extract the imprinted pseudo-template molecule pipemidic acid;

[0031] (c) drying the reacted solid particles in step (b) to obtain a molecularly imprinted polymer with broad-spectrum specificity for quinolones.

Embodiment 2

[0032] Example 2 Molecularly imprinted polymer synthesis example 2

[0033] (a) Put 1 part of pseudomolecular template pipemidic acid, 7 parts of functional monomer methacrylic acid, 30 parts of initiator azobisisobutyronitrile and 30 parts of crosslinking agent ethylene glycol dimethacrylate in In chloroform solvent, react at 70°C for 10 hours to obtain solid particles;

[0034] (b) Put the solid particles in a Soxhlet extractor, and continuously reflux the extraction solution of methanol and acetic acid (9:2, V / V) for 24 hours to extract the imprinted pseudo-template molecule pipemidic acid;

[0035] (c) drying the reacted solid particles in step (b) to obtain a broad-spectrum specific molecularly imprinted polymer of quinolones.

Embodiment 3

[0036] Example 3 Molecularly imprinted polymer synthesis example 3

[0037] (a) Put 1 part of pseudomolecular template pipemidic acid, 6 parts of functional monomer methacrylic acid, 25 parts of initiator azobisisobutyronitrile and 25 parts of crosslinking agent ethylene glycol dimethacrylate in In chloroform solvent, react at 65°C for 11 hours to obtain solid particles;

[0038] (b) Put the solid particles in a Soxhlet extractor, and continuously reflux the extraction solution of methanol and acetic acid (9:0.5, V / V) for 18 hours to extract the imprinted pseudo-template molecule pipemidic acid;

[0039] (c) drying the reacted solid particles in step (b) to obtain a molecularly imprinted polymer with broad-spectrum specificity for quinolone drugs.

[0040] The scanning electron micrographs of the molecularly imprinted polymer prepared in this example are as follows: figure 2 shown by figure 2 It can be seen that its surface is porous, which is a cavity formed by template ...

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PUM

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Abstract

The invention discloses a broad spectrum specific molecularly imprinted polymer of quinolone medicine, a chemiluminescence kit using the broad spectrum specific molecularly imprinted polymer as a recognition element, a detection method and application. The molecularly imprinted polymer uses pipemidic acid as a pseudomolecular template. The synthesized broad spectrum specific molecularly imprintedpolymer can recognize eight kinds of quinolone medicine. The chemiluminescence kit prepared on the basis of the molecularly imprinted polymer can be used for performing multi-residue detection on eight kinds of quinolone medicine in meat tissues; the repeated utilization can be realized; the detection sensitivity is improved; the detection time is shortened; the detection cost is reduced.

Description

technical field [0001] The invention relates to the field of animal food safety, in particular to a molecularly imprinted polymer capable of capturing 8 kinds of quinolone drugs, a chemiluminescence kit prepared therefrom, and a detection method and application of the kit. Background technique [0002] Quinolones are a class of chemically synthesized antibacterial drugs, which have low toxicity, broad antibacterial spectrum, and strong antibacterial activity, and are widely used in the treatment of various bacterial diseases in animals and humans. Common varieties are enrofloxacin, sarafloxacin, ciprofloxacin, lomefloxacin, ofloxacin, pefloxacin, danoxacin, marbofloxacin. With the extensive use of quinolones in food animal breeding, their residues in animal-derived foods have attracted widespread attention. Residues of quinolones may cause long-term ingestion of small amounts by consumers, causing adverse reactions such as blood diseases, osteoporosis, and allergies, and mo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 陈公武翟明成王继英赵元煜耿晓彬张英林
Owner 河北英茂生物科技有限公司
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