Preparation method of immune chromatography test paper for detecting cephalosporin antibiotic

A technology of immunochromatography test paper and antibiotics, which is applied in the field of immunological detection, can solve the problems of not being able to adapt to high-throughput rapid detection, and achieve the effect of easy operation
CN101315364AInactive Publication Date: 2008-12-03JIANGNAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
JIANGNAN UNIV
Publication Date
2008-12-03
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a method for preparing an immunity chromatographic test strip for testing cephalosporins antibiotics, and relates to the immunity testing field. The test strip is composed of a sample pad, a combined pad, a cellulose nitrate membrane, a water absorbing pad and a PVC backing; the sample pad, the combined pad, the cellulose nitrate membrane and the water absorbing pad are sequentially stuck to the PVC backing; the combined pad is coated with a group-specific anti-cephalosporins antibiotic antibody colloidal gold label of anti-cephalosporins antibiotics; and the cellulose nitrate membrane is sequentially coated with a cefalexin-OVA test line and a goat anti-rabbit IgG quality control line. The method prepares the test trip by assembling the test preparation of the combination of the group-specific antibody of the anti-cephalosporins antibiotics and the chromogenic colloidal gold, and carries out a multi-residue screening test of the cephalosporins antibiotics with low cost and rapidness; and the portable test strip which can be used for the cephalosporins antibiotic residue tests of different samples is the application of the immunity chromatographic analysis method.
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Description

technical field

[0001] The invention relates to a preparation method of an immunochromatographic test strip for detecting cephalosporin antibiotics, and the invention relates to the field of immunological detection, in particular to the preparation of an immune colloidal gold reagent and a test strip. Background technique

[0002] Because there are many kinds of veterinary drugs, there are also many kinds of cephalosporin antibiotics, and the single residue detection is far from being able to meet the good detection of drug residues caused by the abuse of veterinary drugs, and for the production of fermented milk, at least one class of antibiotics can be detected. is very useful. Existing methods that can realize multi-residue detection are mainly instrumental analysis, including high performance liquid chromatography, liquid chromatography-mass spectrometry (HPLC-MS), gas chromatography-mass spectrometry (GC-MS), and the like. However, these detection methods require expen...

Claims

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