Ethanol concentration detection kit and manufacture method thereof

Abstract

The invention provides an ethanol concentration detection kit and a manufacture method thereof. The ethanol concentration detection kin is composed of a reagent strip and a standard comparison card, the reagent strip is a filter paper strip with width ranging from 0.5cm to 1cm. Immobilized ethanol dehydrogenase, diaphorase, oxidized iodonitrotetrazolium (INT) and coenzyme are arranged on the filter paper strip. The standard comparison card comprises color developing comparison areas of ethanol with different concentrations. The detection kit is convenient, sanitary, fast and high in sensitivity and stability when being used for detecting ethanol concentration of the drunks, and the detection kit is easy to carry and particularly suitable for on-site detection.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to an ethanol concentration detection kit and a preparation method of the kit. Background technique [0002] In recent years, criminal cases caused by drinking and traffic accidents caused by drunk driving have been on the rise. On May 1, 2011, the "Criminal Law Amendment (VIII) of the People's Republic of China" was officially implemented, and drunk driving was prosecuted as a crime of dangerous driving. People's criminal responsibility, the topic of drunk driving has once again become the focus of attention. At present, many countries have formulated the relationship between blood ethanol concentration and traffic accident responsibility determination, and established a variety of methods that can detect blood ethanol content, such as GM / MS method. The equipment is expensive, the detection cost is high, special training is required, and the shortcomings of being unable to...

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Problems solved by technology

Since the detection of whether the driver is drinking alcohol is measured according to the driver’s exhaled breath, the size of the exhaled pressure directly affects the measurement result. In addition, the breath alcohol content detector is calibrated by the alcohol standard gas in the air, and the alcohol standard in the air The accuracy of the gas value determines the accuracy of the test results. Since ethanol is liquid at room temperature and has a high boiling point (78.5°C), the uniformity of the standard gas prepared with a higher concentration of ethanol is not very good due to incomplete volatilization and vaporization. Ideally, this makes it difficult to guarantee the repeatability of the test of the breath alcohol content detector, and the results of the two tests are very different. At the same time, its price is also very expensive, and it is unhygienic and prone to cross-infection diseases

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