Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ethanol concentration detection kit and manufacture method thereof

A technology for testing kits and ethanol concentration, applied in the field of medical testing, can solve the problems of high price, large differences in testing results, unsanitary, etc., and achieve the effects of improving sensitivity, fast testing speed, and ensuring specificity.

Inactive Publication Date: 2012-06-27
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
View PDF9 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the detection of whether the driver is drinking alcohol is measured according to the driver’s exhaled breath, the size of the exhaled pressure directly affects the measurement result. In addition, the breath alcohol content detector is calibrated by the alcohol standard gas in the air, and the alcohol standard in the air The accuracy of the gas value determines the accuracy of the test results. Since ethanol is liquid at room temperature and has a high boiling point (78.5°C), the uniformity of the standard gas prepared with a higher concentration of ethanol is not very good due to incomplete volatilization and vaporization. Ideally, this makes it difficult to guarantee the repeatability of the test of the breath alcohol content detector, and the results of the two tests are very different. At the same time, its price is also very expensive, and it is unhygienic and prone to cross-infection diseases

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ethanol concentration detection kit and manufacture method thereof
  • Ethanol concentration detection kit and manufacture method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The ethanol concentration detection kit of this embodiment is composed of a reagent strip and a standard comparison card, wherein the reagent strip is a filter paper strip with a width of 0.5 cm, and the filter paper strip contains solid-phase alcohol dehydrogenase, diaphorase, oxidized INT solution and coenzyme, the standard comparison card includes color comparison areas of different ethanol concentrations. The coenzyme is NAD. The standard comparison card includes five color comparison areas with ethanol concentrations of 0 mg / ml, 0.2 mg / ml, 0.8 mg / ml, 1.5 mg / ml and 3.0 mg / ml respectively.

[0027] The preparation method of the ethanol concentration detection kit comprises the preparation of reagent strips and the preparation of standard comparison cards, wherein:

[0028] The preparation of the reagent strip comprises the following steps:

[0029] (1) Preparation of alcohol dehydrogenase and diaphorase solution: dissolve alcohol dehydrogenase and diaphorase in 0.1...

Embodiment 2

[0036] The ethanol concentration detection kit of this embodiment is composed of a reagent strip and a standard comparison card, wherein the reagent strip is a filter paper strip with a width of 0.5 cm, and the filter paper strip contains solid-phase alcohol dehydrogenase, diaphorase, oxidized INT solution and coenzyme, the standard comparison card includes color comparison areas of different ethanol concentrations. The coenzyme is thio-NAD. The standard comparison card includes five color comparison areas with ethanol concentrations of 0 mg / ml, 0.2 mg / ml, 0.8 mg / ml, 1.5 mg / ml and 3.0 mg / ml respectively.

[0037] The preparation method of the ethanol concentration detection kit comprises the preparation of reagent strips and the preparation of standard comparison cards, wherein:

[0038] The preparation of the reagent strip comprises the following steps:

[0039] (1) Preparation of alcohol dehydrogenase and diaphorase solution: dissolve alcohol dehydrogenase and diaphorase i...

Embodiment 3

[0046] The ethanol concentration detection kit of this embodiment is composed of a reagent strip and a standard comparison card, wherein the reagent strip is a filter paper strip with a width of 0.5 cm, and the filter paper strip contains solid-phase alcohol dehydrogenase, diaphorase, oxidized INT solution and coenzyme, the standard comparison card includes color comparison areas of different ethanol concentrations. The coenzyme is NADP. The standard comparison card includes five color comparison areas with ethanol concentrations of 0 mg / ml, 0.2 mg / ml, 0.8 mg / ml, 1.5 mg / ml and 3.0 mg / ml respectively.

[0047] The preparation method of the ethanol concentration detection kit comprises the preparation of reagent strips and the preparation of standard comparison cards, wherein:

[0048] The preparation of the reagent strip comprises the following steps:

[0049] (1) Prepare alcohol dehydrogenase and diaphorase solution: dissolve alcohol dehydrogenase and diaphorase in 0.1M tris...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
boiling pointaaaaaaaaaa
Login to View More

Abstract

The invention provides an ethanol concentration detection kit and a manufacture method thereof. The ethanol concentration detection kin is composed of a reagent strip and a standard comparison card, the reagent strip is a filter paper strip with width ranging from 0.5cm to 1cm. Immobilized ethanol dehydrogenase, diaphorase, oxidized iodonitrotetrazolium (INT) and coenzyme are arranged on the filter paper strip. The standard comparison card comprises color developing comparison areas of ethanol with different concentrations. The detection kit is convenient, sanitary, fast and high in sensitivity and stability when being used for detecting ethanol concentration of the drunks, and the detection kit is easy to carry and particularly suitable for on-site detection.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to an ethanol concentration detection kit and a preparation method of the kit. Background technique [0002] In recent years, criminal cases caused by drinking and traffic accidents caused by drunk driving have been on the rise. On May 1, 2011, the "Criminal Law Amendment (VIII) of the People's Republic of China" was officially implemented, and drunk driving was prosecuted as a crime of dangerous driving. People's criminal responsibility, the topic of drunk driving has once again become the focus of attention. At present, many countries have formulated the relationship between blood ethanol concentration and traffic accident responsibility determination, and established a variety of methods that can detect blood ethanol content, such as GM / MS method. The equipment is expensive, the detection cost is high, special training is required, and the shortcomings of being unable to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/98G01N33/52
Inventor 邹炳德沃燕波
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com