Method for simultaneous SMART column on-line purification and HPLC/UVE fluorescent detection of four aflatoxins in peanut product

A technology for aflatoxin and peanut products, which is applied in the field of food inspection, can solve the problems of increasing the loss of target substances and increasing the detection work time, and achieves the effects of short detection time, high sensitivity and good repeatability

Inactive Publication Date: 2017-02-01
ZHOUSHAN INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

However, the traditional immunoaffinity column method is used in the sample pretreatment technology, which not only increases the detection working time, but also increases the loss of the target during the detection process.

Method used

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  • Method for simultaneous SMART column on-line purification and HPLC/UVE fluorescent detection of four aflatoxins in peanut product
  • Method for simultaneous SMART column on-line purification and HPLC/UVE fluorescent detection of four aflatoxins in peanut product
  • Method for simultaneous SMART column on-line purification and HPLC/UVE fluorescent detection of four aflatoxins in peanut product

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Embodiment

[0025] A method for SMART column online purification-HPLC / UVE fluorescence simultaneous detection of four aflatoxins in peanut products, comprising the following steps:

[0026] (1) Sample treatment: Accurately weigh 20.0 g of the sample, add 2 g of NaCl, and at the same time add 100 mL of extraction solvent (mixed with methanol and water at a volume ratio of 4:1) and 50 mL of n-hexane in a homogenizer and stir evenly. Centrifuge at 10000r / min at 4°C for 10min, take the lower layer with a disposable pipette and filter it with rapid qualitative filter paper, collect the filtrate, take 14mL of the filtrate and add 86mL of 1mM PBS buffer solution (pH7.2) for dilution to obtain the extract.

[0027] (2) On-line purification of SMART immunoaffinity column-HPLC / UVE fluorescence detection: take the extract and put it on SMART immunoaffinity column, rinse, dry, and elute (using mobile phase: water: methanol: acetonitrile volume ratio = 60:30 :15, carry out elution), eluent directly en...

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Abstract

The invention discloses a method for simultaneous SMART column on-line purification and HPLC / UVE fluorescent detection of four aflatoxins in a peanut product. The method comprises the following steps: (1) sample treatment; and (2) SMART immunoaffinity column on-line purification and HPLC / UVE fluorescent detection: loading a SMART immunoaffinity column with liquid extract, carrying out leaching, drying and elution, allowing eluate to directly enter a HPLC chromatographic column, treating liquid having passed through the chromatographic column with a post-UVE column derivatization reactor, then detecting the treated liquid with a fluorescence detector, drafting a standard curve and quantifying the four aflatoxins by using an external standard method according to peak areas. The method is simple in pre-treatment, good in purifying effect, high in sensitivity, good in repeatability and suitable for multi-residue rapid detection of trace aflatoxins in peanut.

Description

technical field [0001] The invention relates to the technical field of food inspection, in particular to a SMART column online purification-HPLC / UVE fluorescence method for simultaneously detecting four aflatoxins in peanut products. Background technique [0002] Aflatoxin (AF for short) is the most toxic mycotoxin found so far, and its toxicity is equivalent to 10 times that of potassium cyanide and 68 times that of arsenic. It can pollute food and feed through various ways, directly or indirectly enter the human food chain, threaten human health and life safety, and seriously damage the internal organs of humans and animals, especially the liver. It inhibits the synthesis of DNA, RNA and liver proteins, resulting in Acute poisoning, there are many reports about aflatoxin poisoning incidents. The toxin is a metabolite of the toxin-producing strains of Aspergillus flavus and Aspergillus parasiticus, and is commonly found in moldy grains and grain products. However, due to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 赵巧灵王萍亚戴意飞蒋玲波
Owner ZHOUSHAN INST FOR FOOD & DRUG CONTROL
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