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Immunochromatography assay for quantitatively detecting organophosphorus pesticides

A technology of quantitative detection and immunochromatography, which is applied in the direction of measuring devices, analytical materials, biological testing, etc., can solve the problems of restricting the general application of such methods and expensive instruments, and achieve multi-residue detection, high sensitivity, and reduce human body health hazard effects

Active Publication Date: 2017-07-21
MEIZHENG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Technical problems to be solved: Traditional instrument-based organophosphorus pesticide detection methods require expensive instruments and require professional operators to perform complex sample pretreatment processes, which limits the general application of such methods

Method used

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  • Immunochromatography assay for quantitatively detecting organophosphorus pesticides
  • Immunochromatography assay for quantitatively detecting organophosphorus pesticides
  • Immunochromatography assay for quantitatively detecting organophosphorus pesticides

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preparation example Construction

[0042] The method for preparing the monoclonal antibody against electric eel acetylcholinesterase of the present invention comprises the following steps:

[0043] Electric eel acetylcholinesterase (purchased from Sigma, CAS: 9000-81-1) was used as an immunogen to immunize Balb / C female mice. Use sterile normal saline to dilute the complete antigen to 1 mg / mL, take an appropriate amount of immunogen diluent, mix it with an equal volume of rapid immune adjuvant, emulsify it evenly with a syringe, and then inject it subcutaneously at multiple points at a dose of 20 μg / cause. Three weeks later, the booster immunization was carried out three times according to the same dose and method. After four times of immunization, blood was collected from the tail of the mice, and the serum was separated by centrifugation. Acetylcholinesterase was used as the detection target, and the titer of the serum was determined by ELISA. Mice with high titers were subjected to cell fusion.

[0044] The...

Embodiment 1

[0051] The quantum dot immunochromatographic test strip detects organophosphorus pesticides, including the following steps:

[0052] (1) Synthesis of CdSe@ZnS quantum dots

[0053] Add 50 mg of selenium powder to 50 mL of NaBH containing 100 mg under nitrogen protection 4 In the aqueous solution, heated to 50°C in an oil bath, condensed and refluxed until the selenium powder was dissolved to obtain a transparent solution, which was used as solvent 1; another 50mg of selenium powder was added to 50mL containing 200mg of Na 2 SO 3 In the aqueous solution, heated to boiling in an oil bath, condensed and refluxed for 5 hours, and a transparent solution was obtained by suction filtration, which was used as solvent 2.

[0054] Under nitrogen protection, 300mg CdCl 2 Dissolve in 80mL of deionized water, add 450mg of thioglycolic acid, adjust the pH of the solution to 9~10, make the solution become colorless and transparent, add solvent 1 and solvent 2 under the protection of nitro...

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Abstract

The invention discloses an immunochromatography assay for quantitatively detecting organophosphorus pesticides. The immunochromatography assay is characterized in that the principle combining the organophosphorus pesticides and acetylcholin esterase is combined with the immunochromatography technology, a monoclonal antibody for resisting electric eel acetylcholin esterase is prepared, the site, combining with the electric eel acetylcholin esterase, of the antibody is identical with the site, combining with the electric eel acetylcholin esterase, of organophosphorus, the monoclonal antibody is used to fix a detection line, the acetylcholin esterase is used to modify an indicator, the immunochromatography assay for quantitatively detecting the organophosphorus pesticides is provided to achieve the multi-residue detection of the organophosphorus pesticides, and the quantitative detection of the organophosphorus pesticides is achieved according to the signal strength of the detection line and a quality control line. The immunochromatography assay has the advantages that the marking of the organophosphorus pesticide indicator and the chemical preparation of protein conjugates are avoided, detection procedures are simplified, human health damage and severe environment pollution caused by the use of standard organophosphorus pesticides are reduced, and the immunochromatography assay is high in detection sensitivity and capable of satisfying the detection requirements of accuracy, quickness and field high throughput.

Description

technical field [0001] The invention belongs to the technical field of pesticide residue detection, and in particular relates to an immunochromatographic method for quantitative detection of organophosphorus pesticides. Background technique [0002] In the process of agricultural production, the use of pesticides plays a very important role. Pesticides can effectively prevent and control pests and diseases. However, while increasing the output of agricultural and sideline products, they also cause serious food and environmental pollution problems. Pesticides that enter the soil with rainwater are finally It will flow into rivers, lakes and seas, which not only damages the environment, but also destroys human drinking water resources, and pesticide residues will accumulate in aquatic products. Human consumption of aquatic products contaminated by pesticides for a long time will cause pesticide poisoning, especially organophosphorus pesticides. why. [0003] Organophosphorus ...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/58
CPCG01N33/558G01N33/587G01N33/588
Inventor 张勋
Owner MEIZHENG BIO TECH CO LTD
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