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A receptor-based immunochromatographic test strip for detecting β-lactam antibiotics and its preparation method

An immunochromatographic test paper and lactam technology, which is applied in the field of immunological detection, can solve the problems of being unable to adapt to high-throughput rapid detection, and achieve the effects of rapid detection and simple operation.

Active Publication Date: 2015-09-23
无锡迪腾敏生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these detection methods require expensive instruments and equipment, professional and technical personnel are required for operation, and cannot be adapted to high-throughput, on-site rapid detection

Method used

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  • A receptor-based immunochromatographic test strip for detecting β-lactam antibiotics and its preparation method
  • A receptor-based immunochromatographic test strip for detecting β-lactam antibiotics and its preparation method

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Embodiment 1

[0018] like figure 1 As shown, one end of the PVC backing 1 is pasted with a sample pad 2 and a nitrocellulose membrane 3 in turn, and the other end is stuck with a water-absorbing pad 4, and the nitrocellulose membrane 3 is coated with ampicillin-BSA as a detection line 5, and goat anti-mouse IgG served as control line 6. The matching microwell plate is coated with anti-β-lactam antibiotic cluster-specific antibody-colloidal gold marker and mouse negative serum-colloidal gold marker.

[0019] Prepare as follows:

[0020] (1) Preparation of ampicillin-BSA conjugate: Dissolve ampicillin and EDC in MES (0.01mol / L, pH 5) at a molar ratio of 1:3, stir and activate at room temperature for 1 hour, and then add the activated substance dropwise to dissolve In BSA-containing HEPES (0.01mol / L, pH 7), the molar ratio of BSA and ampicillin was 1:50, reacted for 4 hours, and dialyzed with normal saline at 4°C for 3 days to form ampicillin-BSA conjugates;

[0021] (2) Preparation of coll...

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Abstract

The invention discloses a receptor-based immunochromatography test strip for detecting Beta-lactam antibiotics and a preparation method of the test strip, which belong to the technical field of immunological detection. The test strip comprises a PVC (polyvinyl chloride) back lining, wherein the front end of the PVC back lining is provided with a sample pad which is connected with the front end of a cellulose nitrate membrane; the rear end of the cellulose nitrate membrane is connected with a water absorption pad; a combined microporous board is enveloped with a mixture of an anti-Beta-lactam antibiotic receptor-colloidal gold marker and a mouse negative serum-colloidal gold marker; the cellulose nitrate membrane is sequentially enveloped with an ampicillin-BSA (Bovine Serum Albumin)) detection line and a goat anti mouse IgG (Intravenous Gamma Globulin) control line. By virtue of the immunochromatography suitable for on-site detection, the multi-residue detection on at least fifteen Beta-lactam antibiotics can be achieved; the detection is fast and only needs 5-10 minutes; the test strip is convenient to carry, suitable for on-site detection, and simple and convenient to operate without needing professionals.

Description

technical field [0001] The invention relates to a receptor-based immunochromatographic test strip for detecting β-lactam antibiotics and a preparation method thereof, belonging to the technical field of immunological detection. Background technique [0002] Because there are many kinds of veterinary drugs, there are also many kinds of β-lactam antibiotics, and the single residue detection is far from being able to satisfy the good detection of drug residues caused by the abuse of veterinary drugs. Using antigen-antibody reaction can realize the detection of a small part of β-lactam antibiotics, but it is still difficult to meet the needs of multi-residue detection. Existing methods that can achieve multi-residue detection are mainly instrumental analysis, including high performance liquid chromatography, liquid chromatography-mass spectrometry (HPLC-MS), gas chromatography-mass spectrometry (GC-MS), and TTC methods. However, these detection methods require expensive instrum...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/532
CPCG01N33/531G01N33/532G01N33/558
Inventor 匡华胥传来郭靖男徐丽广马伟刘丽强宋珊珊吴晓玲
Owner 无锡迪腾敏生物科技有限公司
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