Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Quinolone and sulpha compound extraction method from animal sample and special immuno affinity absorbent

A technology for quinolones and compounds, which is applied in the field of extracting quinolones and sulfonamide compounds, can solve the problems of non-commercialized IAC columns, waste of organic solvents, and complicated processing procedures, and achieves reduction of analysis costs, environmental pollution, and pretreatment processes. Simplified and purifying effect

Inactive Publication Date: 2009-06-17
CHINA AGRI UNIV
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pretreatment of these methods utilizes liquid-liquid distribution, conventional SPE column purification and separation, all of which have shortcomings such as cumbersome treatment process, poor purification effect, waste of organic solvents, and long time required to varying degrees.
Immunoaffinity technology is a new technology that was applied in the field of analysis in the 1990s, but there is no report on the simultaneous purification of quinolones and sulfonamides in the matrix with immunoaffinity columns, and there is no commercial IAC column for sale

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quinolone and sulpha compound extraction method from animal sample and special immuno affinity absorbent
  • Quinolone and sulpha compound extraction method from animal sample and special immuno affinity absorbent
  • Quinolone and sulpha compound extraction method from animal sample and special immuno affinity absorbent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1, the preparation of the immunochromatographic column of purifying quinolones and sulfa compounds

[0035] 1. Preparation of two mouse monoclonal antibodies, norfloxacin and sulfamethoxazole

[0036] 1. Preparation of norfloxacin hapten and sulfamethoxazole hapten

[0037] A, preparation of norfloxacin hapten

[0038] Such as figure 1 shown, including the following steps:

[0039] 1) Take 0.5g norfloxacin (NOR) (H010197, China Veterinary Drug Administration) in a dry 50ml round bottom flask, add dry chloroform 20ml in a nitrogen stream and stir to completely dissolve the raw material, add dropwise 0.5 ml pyridine catalyzed, after mixing evenly, add 1mol / L thionyl chloride dropwise, and stir at room temperature for 3h to obtain the intermediate chlorinated norfloxacin.

[0040] 2) Add 1.2 mol / L 6-amino-n-hexanoic acid to the reaction solution, add 10 ml of pyridine, stir well, raise the temperature of the oil bath to 70° C. and react for 9 hours.

[0041...

Embodiment 2

[0094] Example 2. Preparation of a kit containing an immunochromatographic column coupled with a mouse monoclonal antibody and its purification effect on quinolones and / or sulfonamides

[0095] 1. Preparation of kits containing immunochromatographic columns

[0096] The kit is mainly composed of box body, immunochromatographic column (IAC column), ciprofloxacin standard solution, norfloxacin standard solution, pefloxacin standard solution, ofloxacin standard solution, enoxacin standard solution , standard solution of marbofloxacin, standard solution of lomefloxacin, standard solution of danofloxacin, standard solution of enrofloxacin, standard solution of sarafloxacin, standard solution of difloxacin, standard solution of oxquinic acid, flumequine Standard solution, sulfadiazine standard solution, sulfathiazole standard solution, sulfapyridine standard solution, sulfamethiadiazole standard solution, sulfamethoxazole standard solution, sulfamethoxazole standard solution, washin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method and special immune affinity adsorbent for extracting quinolone compound and / or sulfonamide compound. The immune affinity adsorbent consists of a solid-phase carrier and Norfloxacin monoclonal antibody and / or sulfamethoxazole monoclonal antibody coupled with the carrier, wherein the Norfloxacin monoclonal antibody and the sulfamethoxazole monoclonal antibody are obtained by taking Norfloxacin hapten, sulfamethoxazole hapten and carrier protein conjugate as immunogen; the quinolone compound is at least one of the following 13 types of compounds: Ciprofloxacin, Norfloxacin, Pefloxacin, Ofloxacin, Enoxacin, Marbofloxacin, Lomefloxacin, Danofloxacin, Enrofloxacin, Sarafloxacin, Difloxacin, Oxolinic acid and Flumequine; and the sulfonamide compound is at least one of the following 6 types of compounds: sulfapyridine, sulfathiazole, sulphapyridine, sulfamethizole, sulfamonomethoxine and sulfamethoxazole.

Description

technical field [0001] The invention relates to a method for extracting quinolones and sulfonamide compounds from animal samples and a special immunoaffinity adsorbent thereof. Background technique [0002] With the development of life sciences, people have become more and more interested in the substances and their changes in living organisms, and the analysis of biological samples has become a necessary means to explore and discover the mysteries of life. Due to the complex components of biological samples, the concentration of analytes is low, and most of the sample volumes are small, which puts forward higher requirements for the selectivity and sensitivity of the analytical method. Immunoaffinity chromatography (IAC, Immunoaffinity chromatography) is an analytical method that combines an immune response with a chromatographic analysis method. Its high selectivity and high affinity undoubtedly simplify the analysis process. In the analysis of veterinary drug residues, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01J20/281C07D215/56C07D261/16
Inventor 沈建忠李存张素霞史为民万宇平江海洋曹兴元冯才伟李建成王战辉
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com