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Method for the extraction, enrichment and quantification of trace amounts of sarafloxacin on suspended particulates in water

A technology for suspending particles and water suspension, which is applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2016-03-30
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods are limited to the quantification of dissolved sarafloxacin

Method used

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  • Method for the extraction, enrichment and quantification of trace amounts of sarafloxacin on suspended particulates in water
  • Method for the extraction, enrichment and quantification of trace amounts of sarafloxacin on suspended particulates in water
  • Method for the extraction, enrichment and quantification of trace amounts of sarafloxacin on suspended particulates in water

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Embodiment Construction

[0035] The technical solution of the present invention will be further described below, but it is not limited to this. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the protection scope of the present invention .

[0036] Such as figure 1 As shown, the method for extracting, enriching and quantifying trace amounts of sarafloxacin on suspended particulate matter in water provided by the invention, its specific implementation steps are as follows:

[0037] (1) Take 2000ml of the target water sample, filter it with a 0.45μm microporous fiber membrane, collect the filtered filter membrane, dry the filter membrane, cut it into pieces and put it in the conical flask, measure an appropriate amount of methanol with a measuring cylinder and pour it into the conical flask , the volume ratio of the mass of the suspended parti...

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Abstract

The invention discloses a method for extraction, enrichment and quantification of trace sarafloxacin on suspended particulate matters in water. The method comprises the following steps: filtering a target water sample by using a micro porous fiber membrane; collecting the filtered filter membrane, airing the filter membrane and then cutting into pieces, and putting the filter membrane pieces into a triangular flask; adding an extraction agent, sealing, oscillating and carrying out ultrasonic extraction; filtering extract liquor by using an organic filter membrane, and simultaneously transferring the filtered extract liquor into a K-D concentration bottle; adding a dewatering desiccant to the filtered extract liquor; sucking the moisture, and then putting the K-D concentration bottle into a rotary evaporator and concentrating; purging the concentrated liquid by nitrogen until the volume is smaller than 1ml; enabling the concentrated filtered extract fluid to achieve a constant volume of 1mL, and then transferring the fluid to a special agilent bottle; detecting the fluid by virtue of a combined instrument of high performance liquid chromatography-tandem three-stage mass spectrometry by adopting an internal standard method; and analyzing a chromatography-mass spectrometry analysis graph, thereby finishing detection. According to the method, the content of sarafloxacin adsorbed on the suspended particulate matters in a water environment can be detected, and the blank of antibiotics detection is filled.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a detection method for sarafloxacin in a water environment, in particular to a quantitative method for extracting and enriching sarafloxacin on particulate matter in water. Background technique [0002] Sarafloxacin belongs to the third generation of quinolone antibiotics. It is another new antibacterial drug for livestock and poultry after enrofloxacin. It has a broad-spectrum antibacterial effect and has a significant killing effect on Gram-positive and negative bacteria. , It is also effective against mycoplasma and certain anaerobic bacteria. It is widely used in the treatment of livestock and poultry digestive system, respiratory system, urinary system infection and mycoplasma disease. [0003] It is reported that sarafloxacin is not completely metabolized in animals, and there are still sarafloxacin residues in the excreta, which flow into the water environm...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/08G01N30/02
Inventor 徐勇鹏崔福义
Owner HARBIN INST OF TECH
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