Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for extraction enrichment and quantification of trace norfloxacin on suspended particles in water

A technology for suspended particulate matter and water suspension, used in measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2015-07-08
HARBIN INST OF TECH
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are limited to the quantification of dissolved norfloxacin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extraction enrichment and quantification of trace norfloxacin on suspended particles in water
  • Method for extraction enrichment and quantification of trace norfloxacin on suspended particles in water
  • Method for extraction enrichment and quantification of trace norfloxacin on suspended particles in water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The technical solution of the present invention will be further described below, but it is not limited to this. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the protection scope of the present invention .

[0036] Such as figure 1 As shown, the method for extracting, enriching and quantifying trace amounts of norfloxacin on suspended particulate matter in water provided by the invention, its specific implementation steps are as follows:

[0037] (1) Take 2000ml of the target water sample, filter it with a 0.45μm microporous fiber membrane, collect the filtered filter membrane, dry the filter membrane, cut it into pieces and put it in the conical flask, measure an appropriate amount of methanol with a measuring cylinder and pour it into the conical flask In the above, the volume ratio of the mass of suspende...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
pore sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for extraction enrichment and quantification of trace norfloxacin on suspended particles in water. The method comprises the steps: filtering a target water sample by a microporous fiber membrane, collecting the filter membrane after filtration, air-drying the filter membrane, then cutting into pieces, placing in a triangular flask, adding an extracting agent, sealing, oscillating, and carrying out ultrasonic extraction; filtering the extracted liquid by an organic filter membrane, and at the same time, transferring the filtered extracted liquid to an K-D concentration bottle; adding a dehydrating drying agent to the extracted filtrate, absorbing up water, then putting the K-D concentration bottle into a rotary evaporator, and concentrating; blowing the concentrated liquid with nitrogen gas until the volume is below 1 mL; making the concentrated extracted filtered liquid to a constant volume of 1 mL, then transferring to an Agilent special flask; adopting an internal standard method, and detecting by a high performance liquid chromatography-tandem three-grade mass spectrometry instrument; and analyzing a chromatographic mass spectrometric analysis diagram, and thus completing the detection. The method supplements the content of norfloxacin adsorbed on the suspended particles in the water environment, and supplements a blank of antibiotic detection.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a detection method for norfloxacin in water environment, in particular to a quantitative method for extraction and enrichment of norfloxacin on particulate matter in water. Background technique [0002] Norfloxacin is a third-generation fluoroquinolone antibiotic drug widely used in livestock. It has spectrum antibacterial properties and has obvious antibacterial effects on Gram-negative bacteria and positive bacteria. Therefore, it is widely used in livestock breeding and water plant breeding. However, ciprofloxacin, one of the metabolites of norfloxacin, still has a strong antibacterial effect, and has strong antibacterial activity against almost all pathogenic bacteria in aquatic organisms. [0003] There are reports that norfloxacin is not completely metabolized in animals, and there are still residues of norfloxacin in the excreta, which flow into the water e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/08
Inventor 徐勇鹏崔福义
Owner HARBIN INST OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com