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Method for the extraction, enrichment and quantification of trace enrofloxacin on suspended particulates in water

A technology for suspending particles and water suspension, which is applied in the direction of measuring devices, instruments, scientific instruments, etc.

Active Publication Date: 2016-06-22
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

These methods are limited to the quantification of dissolved enrofloxacin

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  • Method for the extraction, enrichment and quantification of trace enrofloxacin on suspended particulates in water
  • Method for the extraction, enrichment and quantification of trace enrofloxacin on suspended particulates in water
  • Method for the extraction, enrichment and quantification of trace enrofloxacin on suspended particulates in water

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Embodiment Construction

[0035] The technical solution of the present invention will be further described below, but it is not limited to this. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be covered by the protection scope of the present invention .

[0036] Such as figure 1 As shown, the method for the extraction, enrichment and quantification of trace enrofloxacin on suspended particles in water provided by the invention, its specific implementation steps are as follows:

[0037] (1) Take 2000ml of the target water sample, filter it with a 0.45μm microporous fiber membrane, collect the filtered filter membrane, dry the filter membrane, cut it into pieces and put it in the conical flask, measure an appropriate amount of methanol with a measuring cylinder and pour it into the conical flask In the above, the volume ratio of the mass of suspended partic...

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Abstract

The invention discloses a method for extracting, enriching and quantifying trace amounts of enrofloxacin on suspended particles in water. The target water sample is filtered with a microporous fiber membrane, the filtered membrane is collected, dried and then cut into Put the fragments in a conical flask, add extractant to seal, oscillate, and extract with ultrasonic waves; filter the extract with an organic filter membrane, and transfer the filtered extract to a K-D concentrator bottle; add dehydration desiccant to the extraction filtrate, and blot dry After removing water, put the K-D concentration bottle into a rotary evaporator for concentration; purge the concentrated liquid with nitrogen to a volume of less than 1ml; dilute the extract and filtrate of the concentrated solution to 1mL and transfer it to an Agilent special bottle; In the internal standard method, a high-performance liquid chromatography tandem tertiary mass spectrometer is used for detection; the detection is completed by analyzing the chromatographic mass spectrometry diagram. This method supplements the content of enrofloxacin adsorbed on the suspended particles in the water environment, and supplements the blank of antibiotic detection.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring, and relates to a method for detecting enrofloxacin in a water environment, in particular to a quantitative method for extracting and enriching enrofloxacin on particulate matter in water. Background technique [0002] Enrofloxacin is a third-generation fluoroquinolone antibiotic drug widely used in livestock. It has spectrum antibacterial properties and has obvious antibacterial effects on Gram-negative bacteria and positive bacteria. Therefore, it is widely used in livestock breeding and water plant breeding. However, ciprofloxacin, one of the metabolites of enrofloxacin, still has a strong antibacterial effect, and has strong antibacterial activity against almost all pathogenic bacteria in aquatic organisms. [0003] It is reported that enrofloxacin is not completely metabolized in animals, and there are still enrofloxacin residues in the excreta, which flow into the water env...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/08G01N30/02
Inventor 徐勇鹏崔福义
Owner HARBIN INST OF TECH
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