Hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of monoclonal antibodies thereof

Abstract

The invention discloses a hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of the monoclonal antibodies thereof. Ciprofloxacin (CIP) coupled with bovine serum albumin is used as an antigen to immunize BALB / c mice and cell fusion, screening and cloning are carried out so as to obtain one hybridoma cell strain 1F1 capable of stable passage and secretion of monoclonal antibodies (MAb) to quinolones, wherein, the accession number of the hybridoma cell strain 1F1 is CGMCC No. 5608. The titres of ascitic fluids of the 1F1 monoclonal antibodies are up to 10<-7>, and the type and the subclass of the monoclonal antibodies are IgG1 and kappa chain. According to indirect competitive ELISA analysis, the 1F1 monoclonal antibodies perform specific reactions to quinolones like ciprofloxacin, enrofloxacin, ofloxacin, danofloxacin, norfloxacin, enoxacin, marbofloxacin, sarafloxacin and difloxacin. An ELISA method, a kit and test paper for detecting residual of quinolones in food are developed by using the 1F1 monoclonal antibodies.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell line secreting anti-quinolone monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Fluoroquinolones (Fluoroquinolones, FQs) are a class of synthetic broad-spectrum, highly effective antibacterial drugs. As a veterinary drug and feed additive, it is widely used in animals, resulting in its residues in animal edible products. Such residues can directly cause harm to the human body: causing cartilage toxicity, joint pain and swelling, skin allergic reactions, leukocyte and thrombocytopenia, eosinophilia, hemolysis, liver and kidney damage, etc. What's more serious is that low concentration of residual drugs may produce drug resistance to FQs drug-sensitive pathogenic bacteria, thus indirectly threatening human health. For this reason, many countries have set strict limit standards for quinolone antibiotics. The maximum resi...

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Problems solved by technology

[0004] At present, there are no immunological kits and test strips for detecting quinolone drug residues at home and abroad. The patent application of the invention uses the principle of competitive inhibition ELISA, and uses the monoclonal antibody of quinolone drugs as the core to establish a competitive ELISA method for detecting quinolone drug residues. With independent intellectual property rights, cheap and sensitive quinolone drug residue detection kit, the principle of immunocolloidal gold is applied, and the colloidal gold-labeled ciprofloxacin-specific monoclonal antibody is used as the core to develop a high-sensitivity kit for the rapid detection of quinolone drug residues. Immunity test strips

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