109 results about "Norfloxacino" patented technology

The invention discloses a hybridoma cell strain capable of secreting monoclonal antibodies to quinolones and application of the monoclonal antibodies thereof. Ciprofloxacin (CIP) coupled with bovine serum albumin is used as an antigen to immunize BALB/c mice and cell fusion, screening and cloning are carried out so as to obtain one hybridoma cell strain 1F1 capable of stable passage and secretion of monoclonal antibodies (MAb) to quinolones, wherein, the accession number of the hybridoma cell strain 1F1 is CGMCC No. 5608. The titres of ascitic fluids of the 1F1 monoclonal antibodies are up to 10<-7>, and the type and the subclass of the monoclonal antibodies are IgG1 and kappa chain. According to indirect competitive ELISA analysis, the 1F1 monoclonal antibodies perform specific reactions to quinolones like ciprofloxacin, enrofloxacin, ofloxacin, danofloxacin, norfloxacin, enoxacin, marbofloxacin, sarafloxacin and difloxacin. An ELISA method, a kit and test paper for detecting residual of quinolones in food are developed by using the 1F1 monoclonal antibodies.

The invention discloses a berberine azole compound shown in general formulas such as I-IV, and a pharmaceutically acceptable salt thereof, and also discloses a preparation method of the compound, which comprises the following steps that: a compound VI and an azole compound HIm or 1H-2-mercaptobenzimidazole are reacted to obtain an intermediate VII or VIII, and then the obtained intermediate is reacted with a compound V to obtain the berberine azole compound shown in general formulas such as I or II; and the compound V and a compound IX or XI are reacted to obtain an intermediate X or XII, and then the obtained intermediate is reacted with the azole compound Him to obtain the berberine azole compound shown in general formulas such as III or IV. The berberine azole compound has a certain inhibitory activity on Gram-positive bacteria, Gram-negative bacteria and fungi, the anti-bacterial activity of part of the compounds is equal to or even stronger than that of chloramphenicol or norfloxacin, the antifungal activity of part of the compounds is equal to or even stronger than that of fluconazole, and the berberine azole compound can be used for preparing anti-microbial drugs.

The invention provides an orally-disintegrating micropowder tablet for treating bacterial diarrhea of a young livestock. The orally-disintegrating micropowder tablet comprises the following components in parts by weight: 2-10 parts of norfloxacin, 5-30 parts of a liquorice extract and 25-120 parts of an orally-disintegrating auxiliary material; preferably, the orally-disintegrating micropowder tablet further comprises 0.02-0.5 part of scopolamine hydrobromide, 0.06-3 parts of potassium chloride, 0.1-2 parts of magnesium stearate, 0.01-1 part of sodium dodecyl sulfate, 0.01-0.5 part of sodium glycyrrhizinate, 0.01-0.5 part of cream essence or fresh milk essence and 0.05-0.5 part of sodium glutamate. The invention further provides a preparation method. The preparation method comprises the following steps: firstly, mixing the norfloxacin and the liquorice extract; secondly, dissolving the scopolamine hydrobromide, the potassium chloride and the sodium glutamate; thirdly, mixing the above substances into a paste; finally, adding other substances to prepare the orally-disintegrating micropowder tablet. The orally-disintegrating micropowder tablet can avoid the young livestock from generating a stress reaction, has a good sterilizing effect and can control bacterial toxin; the components can generate synergy to improve the therapeutic effect.

The invention discloses a method for determining 10 quinolone antibiotics in bean sprouts by an ultra-high performance liquid chromatography-tandem mass spectrometry, which is characterized by comprising the following steps of: preprocessing; preparing a standard solution; obtaining a liquid chromatography mass spectrogram and a regression equation; and analyzing and detecting samples by the ultra-high performance liquid chromatography-tandem mass spectrometry. A ultra-high performance liquid chromatography-electrospray tandem mass spectrometry is adopted in this study to carry out an analysisand determination of enrofloxacin, ciprofloxacin, norfloxacin, pefloxacin, ofloxacin, sarafloxacin, danofloxacin, sparfloxacin, fleroxacin and lomefloxacin in the bean sprouts. Sample extraction conditions, purification means, liquid chromatography-mass spectrum parameters and the like are optimized and the matrix effect of the method is evaluated. The result shows that an establishment of the method solves the problem of a simultaneous determination of various quinolone antibiotics in the bean sprouts and provides a technical reference for risk assessment and government supervision.

The invention provides a novel flavonoid glycoside and isoquinoline alkaloid complex structure, preparation, antibacterial application and three complex carrier-free nano-drugs with self-assembly performance. The structural general formula of the complex is shown in the specification, the complex has excellent selective antibacterial ability, and the activity of endophyte escherichia coli and probiotics bacillus subtilis and enterococcus faecium is not influenced while pathogenic bacteria staphylococcus aureus is killed; meanwhile, the compound has a good inhibition effect on multiple drug-resistant staphylococcus aureus, and is superior to norfloxacin, oxacillin, tetracycline and ciprofloxacin. The nano-drug formed by self-assembling the three complexes is uniform in form and good in dispersity and stability, gel or fiber is formed under the condition that auxiliary materials are not needed, and support is provided for development of a carrier-free pure drug delivery preparation.

The invention discloses a method and special immune affinity adsorbent for extracting quinolone compound and/or sulfonamide compound. The immune affinity adsorbent consists of a solid-phase carrier and Norfloxacin monoclonal antibody and/or sulfamethoxazole monoclonal antibody coupled with the carrier, wherein the Norfloxacin monoclonal antibody and the sulfamethoxazole monoclonal antibody are obtained by taking Norfloxacin hapten, sulfamethoxazole hapten and carrier protein conjugate as immunogen; the quinolone compound is at least one of the following 13 types of compounds: Ciprofloxacin, Norfloxacin, Pefloxacin, Ofloxacin, Enoxacin, Marbofloxacin, Lomefloxacin, Danofloxacin, Enrofloxacin, Sarafloxacin, Difloxacin, Oxolinic acid and Flumequine; and the sulfonamide compound is at least one of the following 6 types of compounds: sulfapyridine, sulfathiazole, sulphapyridine, sulfamethizole, sulfamonomethoxine and sulfamethoxazole.

The invention provides an scFv (single chain variable fragment) antibody used for detecting norfloxacin, which comprises a heavy chain and a light chain, wherein the heavy chain and the light chain are connected by a flexible linking peptide (Gly4Ser)3, and the amino acid sequences of the heavy chain and the light chain are separately shown in SEQ ID No. 1 and 2. The invention also provides a gene encoding the scFv antibody. The scFv (single chain variable fragment) antibody used for detecting norfloxacin provided by the invention mainly utilizes a competitive Elisa method to quantitatively or qualitatively detect the residue of norfloxacin in a sample, has low pre-treatment requirement on the sample and simple pre-treatment process, and can simultaneously and rapidly detect large quantity of samples; and after first library building, the scFv antibody is simple and convenient to prepare. A main reagent is provided in a working liquid form for using high-specificity anti-norfloxacin scFv antibody, and the detection method is convenient and feasible and has the characteristics of high specificity, high sensitivity, high precision, high accuracy and the like.

Belonging to the field of pharmacy, the invention relates to a drug resistant Staphylococcus aureus combined drug and application. The drug resistant Staphylococcus aureus combined drug involved in the invention is composed of isoflavone and norfloxacin, wherein the natural compound isoflavone can be separated from plants, is especially high in content in leguminous plants, and has no obvious toxic or side effect. The result of bacteriostatic experiments shows that for norA gene containing fluoroquinolone resistant methicillin-resistant Staphylococcus aureus SA1199B, the isoflavone itself has no bacterial growth inhibiting effect, but after compatibility of the isoflavone and the antibiotic norfloxacin in a ratio of 1:1, the formed combined drug can reduce the minimum bacteriostatic concentration value of norfloxacin by 4 times, and has the same synergistic ability to that of the positive reference substance reserpine. The combined drug provided by the invention can lower the minimum bacteriostatic concentration of norfloxacin, generate an drug-resistant Staphylococcus aureus synergistic effect for norfloxacin, and can be further prepared into drug-resistant Staphylococcus aureus drugs or pharmaceutical preparations for external use.

The invention belongs to the technical field of pharmacy and discloses a method for synthesizing pefloxacin mesylate by utilizing solid formaldehyde instead of liquid formaldehyde. The method is characterized in that solid formaldehyde, methanoic acid and norfloxacin are methylated under a given temperature condition, an intermediate product is crystallized and re-crystallized in 83% ethanol solution after directly having the salt forming reaction with methanesulfonic acid without being separated to obtain the pefloxacin mesylate containing two crystal waters, and the yield can reach 93.2 percent. By adopting the solid formaldehyde, transportation and storage are simple and convenient, the consumption of the formaldehyde is reduced, and economical and environment-friendly effects are achieved; the separation process of the intermediate product is omitted, so that the loss of the intermediate product is reduced, the product yield is increased, the method is applicable to the industrial production, and the quality conforms to the pharmacopeia 2010 version.

The invention mainly discloses a chemical preparation method of norfloxacin. The chemical preparation method is performed by carrying out reaction on 1-ethyl-6-fluoro-7-chloro-4-oxo-1,4-dihydro-quinoline-3-carboxylic acid (formula I) and piperazine (formula II) used as raw materials, Lewis acid used as a catalyst and alcohol used as a solvent at a certain temperature for a certain period of time, and then carrying out post-treatment to obtain norfloxacin. The chemical preparation method disclosed by the invention has the advantages of mild reaction conditions, high reaction selectivity, and extremely low ratio of a 6-fluoro-substituted isomer, and is a very competitive synthesis route.

The invention provides a preparation method of blue latex microsphere labeled antibody and the test strip thereof. The present invention selects norfloxacin as the target object, and adds norfloxacin antibody to the blue latex microsphere solution to obtain Microsphere-labeled antibody, established a blue latex microsphere-labeled immunochromatographic analysis method for the detection of norfloxacin, and applied it to the visual detection of norfloxacin residues in animal-derived foods. The preparation method of the blue latex microsphere-labeled antibody of the present invention and the test strip thereof have the following advantages: (1) in the preparation of the blue latex microsphere-labeled antibody of the present invention, the labeling material used is blue The cost of latex microspheres is much lower than that of chloroauric acid, which reduces the cost of existing pesticide detection. (2) The test strip of the present invention uses less antibody, which also saves the cost. (3) The latex microspheres used in the present invention have the characteristics of good dispersibility, narrow particle size distribution, and bright colors.

The intention discloses a preparation method of a double-ligand zirconium MOFs adsorbent, and the method comprises the following steps: 1) uniformly mixing zirconium nitrate, 2-aminoterephthalic acid,2-hydroxyterephthalic acid, acetic acid and N, N-dimethylformamide, and continuously stirring for 2-4 hours to obtain an orange red solution; 2) transferring the orange red solution into a reaction kettle to react for 20-25 hours at the reaction temperature of 140-160 DEG C; and 3) after the reaction, filtering, washing and drying to obtain faint yellow double-ligand zirconium MOFs solid powder.The double-ligand zirconium MOFs adsorbent has good norfloxacin adsorption performance, and in 100 mL of water containing norfloxacin antibiotics with the initial concentration of 35 mg/L, the adsorption amount of the double-ligand zirconium MOFs adsorbent to norfloxacin is 213.34 mg/g, and the adsorption amount of the double-ligand zirconium MOFs adsorbent to the norfloxacin antibiotics is 213.34mg/g. The adsorption capacity is kept at 70% after the adsorbent is repeatedly used for 5 times, and the adsorbent has great significance in removal and application of antibiotics.

The invention discloses a new norfloxacin compound crystal form, and a preparation method, a preparation and application thereof. The new crystal form is named as a D crystal form; a diffraction angle 2 theta of an X-ray powder diffraction pattern of the norfloxacin D crystal form displays characteristic diffraction peaks at positions of 8.8+/-0.2, 9.8+/-0.2, 15.7+/-0.2, 16.1+/-0.2, 16.5+/-0.2, 17.8+/-0.2, 18.9+/-0.2, 19.4+/-0.2, 20.7+/-0.2, and 21.0+/-0.2. The norfloxacin D form provided by the invention has favorable dissolution rate and stability. The preparation method provided by the invention has the advantages of high operability, safety and environmental protection, and is suitable for industrialized production.

The invention provides a time-resolved fluorescent immunoassay test strip for quantitative determination of tetracycline and norfloxacin, and belongs to the technical field of immunological detection.The test strip sequentially comprises a sample adding area, a binding area and a detection area, wherein the detection area comprises a first detection line, a second detection line and a quality control line in sequence; the first detection line is coated with a tetracycline artificial antigen or norfloxacin artificial antigen, the second detection line is coated with a norfloxacin artificial antigen or tetracycline artificial antigen, and the binding region is tiled with Eu-time-resolved fluorescent nanosphere-labeled antibody Eu-tetracycline monoclonal antibody and Eu-norfloxacin monoclonal antibody. The time-resolved fluorescent immunoassay test strip for the quantitative determination of tetracycline and norfloxacin can quickly quantify tetracycline and norfloxacin in samples with low detection limits, high sensitivity and good repeatability.

The invention provides a preparation method of a soluble black carbon-iron oxide coprecipitation Fenton-like catalyst, and an application of the catalyst in catalytic degradation of norfloxacin. The synthesis method comprises the following steps: 1, preparing black carbon; 2, extracting soluble black carbon; 3, carrying out coprecipitation preparation of the soluble black carbon-iron oxide: taking2-(N-morpholine)ethanesulfonic acid as a buffer solution, adding the soluble black carbon according to a final carbon-iron molar ratio of 0.2-3, adjusting the pH value to 6, putting the obtained system into a shaking table until Fe<2+> in the system is completely oxidized into Fe<3+>, centrifugally collecting the obtained solid-phase product, and washing and freeze-drying the solid-phase productto obtain the soluble black carbon-iron oxide coprecipitation material with different carbon-iron ratios. The prepared soluble black carbon-iron oxide coprecipitation Fenton-like catalyst is applied to treatment of norfloxacin-containing wastewater, and has the advantages of high reaction rate, high degradation efficiency and high reusability. The preparation method has the advantages of wide material sources, simple material preparation process, and facilitation of promotion and application in the actual wastewater treatment process.

The invention discloses stable isotope labeled norfloxacin and a synthesis method thereof. Stable isotope labeled bromoethane used as a stable isotope labeling source reacts with 6, 7-difluoro-4-hydroxyquinoline-3-carboxylic acid ethyl ester to obtain stable isotope labeled 6, 7-difluoro-1-ethyl-1, 4-dihydro-4-oxoquinoline-3-carboxylic acid ethyl ester, hydrolysis reaction is carried out to obtainstable isotope labeled 6, 7-difluoro-1-ethyl-1, 4-dihydro-4-oxoquinoline-3-carboxylic acid, and finally the stable isotope labeled 6, 7-difluoro-1-ethyl-1, 4-dihydro-4-oxoquinoline-3-carboxylic acidreacts with piperazine under an alkaline condition to obtain a stable isotope labeled norfloxacin product. The stable isotope labeling raw material used in the method is cheap and easy to obtain, thesynthesis process is simple, the product is easy to separate and purify, the chemical purity and isotope abundance of the obtained product both reach 99% or above, and the requirement for serving as astandard reagent for quantitative detection of norfloxacin is met; and the use value is high, and good economy is achieved.

The invention belongs to the pharmaceutical field, relates to beta,beta-dimethyl-acry-lalkannin and an application in preparing medicines for inhibiting drug-resistant bacteria. The invention can provide a natural inhibitor for inhibiting efflux pump multidrug methicillin-resistant staphylococcus aureus. The compound has good inhibiting effect on multidrug staphylococcus aureus resistance strain with NorA efflux pump protein and the antibacterial effect is 13 times of positive contrastive drug norfloxacin. The compound can both inhibit tetracycline-resistant staphylococcus aureus strain Xu212with TetK tetracycline efflux pump protein and macrolide-resistant staphylococcus aureus strain RN4220 with MsrA macrolide efflux pump protein, and the minimal inhibitory concentration is 10.8mu m. The compound of the invention can be prepared to medicines for inhibiting multidrug resistance bacteria and produced to antibacterial injection or external medicine preparation.

The invention discloses hybridoma WXX-1 capable of secreting an anti-lomefloxacin monoclonal antibody and an application thereof, and belongs to the technical field of immunochemistry. A murine monoclonal cell strain WXX-1 is prepared by a conventional cell fusion technique, and has reserved in China General Microbiological Culture Collection Center, with a preservation number of CGMCC No.13088. The monoclonal antibody secreted by the cell strain is measured by using an indirect competitive-euzymelinked immunosorbent assay method; IC50 of the antibody to lomefloxacin, norfloxacin and enoxacin are 0.19microgram/L, 0.17microgram/L and 0.23microgram/L, respectively. Recovery ranges of lomefloxacin, norfloxacin and enoxacin in milk are 98.02-107.40%, 82.175%-105.55% and 92.47%-105.52%, respectively. The hybridoma provided by the invention provides materials for immunodetection of residual quinolone antibiotics in food, and has an actual application value.

The invention, belonging to the field of medicine, relates to an aspidinol compound and an application of the compound in inhibiting the activity of multidrug-resistant Staphylococcus aureus (MRSA). According to the invention, the aspidinol compound having the activity of drug-resistant bacteria is obtained by extracting and separating Hypericum monogynum, and the compound is represented as aspidinol C and D in the formula I. The bacteriostasis experiment shows the aspidinol C and D have a certain inhibiting effect on drug-resistant bacterial strains of six different mechanisms, wherein, the compound has a stronger inhibitory activity against MRSA strain SA-1199 B having fluoroquinolone outer pump protein NorA resistance than norfloxacin as a contrast medicine. The compound can be used for preparing antibiotic medicinal preparations. In the formula I, R represents lower alkyl: C1-C5 straight chain branched chain alkyl, such as methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, t-butyl, pentyl, isopentyl, and neo-pentyl.

The invention belongs to the technical field of composite materials, and relates to a preparation method of a lysine functionalized layered double hydroxide (Ly@FeZn) adsorbent. The method comprises the following steps: dissolving iron salt and zinc salt in deionized water to form a solution, adjusting the pH value to 10-11 by NaOH, slowly adding lysine, and uniformly stirring; and sealing the mixed solution in an autoclave lined with polytetrafluoroethylene, carrying out hydrothermal reaction at 60-120 DEG C for 12-24 hours, taking out, centrifuging, washing the solid with deionized water, and carrying out drying at 60 DEG C for 8-16 hours to obtain the product. The prepared Ly@FeZn adsorbent can be applied to adsorption of antibiotics. The preparation method is simple, the process conditions are controllable, and the raw material cost is low. Experiments show that the prepared Ly@FeZn adsorbent can efficiently remove high-concentration antibiotics, especially ciprofloxacin and norfloxacin. The removal rates of 50 mg of the Ly@FeZn adsorbent on ciprofloxacin and norfloxacin (50 mL 200 mg/L) are as high as 98.48% and 95.05% respectively. The adsorbent is expected to be applied and popularized in removing antibiotics in water.

The invention provides a black phosphorus colorimetric/photo-thermal dual-mode visual immunochromatographic detection method for rapid detection of norfloxacin, and belongs to the technical field of immunochromatographic test strip detection. A test strip adopted by the detection method comprises a bottom plate, and a sample adding pad, a combination pad, a nitrocellulose membrane and a water absorption pad which are sequentially adhered to the bottom plate, wherein the nitrocellulose membrane comprises a detection area and a quality control area; and black phosphorus nanosheets are dropwise added into the sample adding pad. The BP nanosheet dropwise added into the sample adding pad is used as a colorimetric/photo-thermal display agent. Colorimetric/photo-thermal data can be obtained by collecting and analyzing heat at the T line, and the detection accuracy is improved through the colorimetric/photo-thermal double-reading method.