Sarafloxacin immune colloidal gold detection card and preparation method thereof

A technology of colloidal gold and detection cards, which is applied to measuring devices, instruments, scientific instruments, etc., to achieve accurate results and simple methods

Inactive Publication Date: 2018-08-17
ZHENJIANG XIANCHUANG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the above situation, the object of the present invention is to provide a kind of immune colloidal gold detection card of sarafloxacin and preparation method thereof in order to overcome the defects existing in the prior art, which can effectively solve the problem that sarafloxacin can be detected quickly and easily.

Method used

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  • Sarafloxacin immune colloidal gold detection card and preparation method thereof
  • Sarafloxacin immune colloidal gold detection card and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] figure 1 , figure 2 Shown embodiment: among the figure 9 is a PVC rubber sheet; 7 is a sample pad; 8 is a colloidal gold binding pad, and the colloidal gold binding pad is coated with a monoclonal antibody colloidal gold marker; 10 is a coating film, i.e. nitrocellulose Plain membrane, the nitrocellulose membrane is coated with sarafloxacin-BSA and goat anti-mouse IgG; 11 is a water-absorbing pad, made of water-absorbing materials such as filter paper.

[0022] On one end (sample end) of the PVC rubber plate 9, the sample pad 7 and the bonding pad 8 are adhered, and the sample pad 7 and the bonding pad 8 are in a side-by-side structure.

[0023] A nitrocellulose membrane 10 is adhered in the middle of the PVC rubber sheet 9 . Goat anti-mouse IgG quality control line 3 and sarafloxacin-BSA detection line 2 are set on the nitrocellulose membrane 10 .

[0024] At the other end of the PVC rubber sheet 9, a water-absorbing pad 11 is adhered. One end of the nitrocellulos...

Embodiment 2

[0026] The preparation of the sarafloxacin colloidal gold detection card is specifically realized by the following steps:

[0027] Preparation of colloidal gold solution: Take a 1 L Erlenmeyer flask, add 495 mL of ultrapure water, and then add 1% chloroauric acid (HAuCl 4 ·3H 2 (0) 5 mL, prepared into 500 mL 0.01% chloroauric acid aqueous solution, heated and boiled, then added 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) Solution 5-7 mL, continue to stir and heat, when the color of the solution turns completely transparent purple red, stop heating after maintaining for 5 min, add water to the original volume, cool to room temperature, and store at 2-8°C for later use;

[0028] Antibody pretreatment: centrifuge the sarafloxacin antibody to be labeled at 1000 r / min, 4°C for 20 min, take the supernatant, and dilute it to 1 mg / mL with 0.01 mol / L PBS; or use 0.01 mol / L Dilute to 1 mg / ml in PBS, pass through a 0.22 µm filter membrane;

[0029] Preparation of colloidal gold...

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Abstract

The invention provides a sarafloxacin immune colloidal gold detection card and a preparation method thereof, and relate to the technical field of animal source food veterinary drug residue detection.According to the present invention, the test paper strip in the detection card shell comprises a PVC glue plate, a sample pad, a colloidal gold bond pad, a coating film and a water absorption pad, wherein the colloidal gold film is a glass cellulose film containing a sarafloxacin monoclonal antibody, the coating film is a nitrocellulose membrane and is provided with a T line and a C line, the T line is coated with a sarafloxacin protein conjugate, and the C line is coated with a goat anti-mouse IgG antibody; and the sarafloxacin immune colloidal gold detection card is effectively used for therapid detection of sarafloxacin, and has characteristics of convenience, rapidness and accurate result.

Description

technical field [0001] The invention relates to the technical field of pesticide residue detection in cereal foods, in particular to an immune colloidal gold detection card for sarafloxacin and a preparation method thereof. Background technique [0002] Sarafloxacin is a fluoroquinolone antibiotic with the following characteristics: Due to its unique chemical structure, it is a very important antibiotic in veterinary clinical medicine. Sarafloxacin has broad-spectrum antibacterial activity against Gram-negative bacteria, Gram-positive bacteria and mycoplasma-like pathogenic bacteria. It also has a bactericidal effect on bacterial mycoplasma at a certain concentration, and can also kill bacteria that are resistant to antibiotics. , such as anti-p-lactobactin, aminoglycoside and so on. Because of its unique effects, sarafloxacin has been widely used in the prevention and treatment of diseases in animals, fish and shrimp since the 1980s. However, recent studies have found tha...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/532
CPCG01N33/577G01N33/532
Inventor 洪霞杜霞刘静
Owner ZHENJIANG XIANCHUANG BIOTECH CO LTD
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