Method for detecting residue of four fluoroquinolone drugs in eggs

A technology of fluoroquinolones and drugs, which is applied to the field of detection of four fluoroquinolone drug residues in eggs, can solve the problems of poor chromatographic separation, cumbersome process, and large consumption of consumables, achieving low cost, high efficiency, accuracy, The effect of ensuring the recovery rate

Inactive Publication Date: 2018-04-24
王安波
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Problems solved by technology

[0003] To sum up, the problems existing in the existing technology are: the existing methods for detecting FQNs residues in eggs are cumbersome, the detection time is long, the chromatographic separation effect is not good, the recovery rate is low, the consumption of consumables is large, and the cost is high

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  • Method for detecting residue of four fluoroquinolone drugs in eggs
  • Method for detecting residue of four fluoroquinolone drugs in eggs
  • Method for detecting residue of four fluoroquinolone drugs in eggs

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Embodiment Construction

[0027] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0028] The application principle of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0029] like figure 1 As shown, the method for detecting 4 kinds of fluoroquinolone drug residues in eggs provided by the embodiment of the present invention comprises the following steps:

[0030] S101: Weigh 2±0.05g uniform egg sample, place it in a centrifuge tube, add 20.0mL phosphoric acid-acetonitrile extract accurately, stir well, vortex mix for 10min, refrigerate and centrifuge at 9000r / min for 10min; transfer the supernatant to another a centrifuge tube;

[0031] S102: Add ...

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Abstract

The invention belongs to the technical field of detection, and discloses a method for detecting the residue of four fluoroquinolone drugs in eggs. The method comprises the following steps: selecting phosphoric acid-acetonitrile as an extraction solution; selecting a degreasing method of water saturated n-hexane; selecting a Waters Oasis HLB column as a solid extraction column; selecting one fourthof extraction solution to pass through the column; selecting a flow phase ratio of 89 percent (0.05mol / L phosphoric acid / triethylamine): 11 percent (acetonitrile); wherein the retention time: enrofloxacin: 8.41+ / -0.5 min, ciprofloxacin: 5.41+ / -0.5 min, danofloxacin: 7.33+ / -0.5 min, and sarafloxacin: 12.53+ / -0.5 min. By adopting the method, the pretreatment time and consumable cost can be greatlysaved, the sensitivity and the recovery rate conform to the current veterinary drug residue detection standard in China, and the method is suitable for the mass sample analysis.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a method for detecting four kinds of fluoroquinolone drug residues in eggs. Background technique [0002] Fluoroquinolones (FQNs) are a class of synthetic antibacterial drugs with strong antibacterial effects. Among them, Enrofloxacin (ENR), Ciprofloxacin (CIP), Danofloxacin (Danofloxacin , DAO) and Sarafloxacin (SAR) are widely used in livestock and poultry production. Drug residues and potential carcinogenicity caused by improper use of FQNs are a serious threat to human health. The Food and Agriculture Organization of the United Nations, the European Union, and my country have all stipulated the amount of FQNs residues in animal-derived foods, and the problems caused by FQNs residues have also attracted more and more attention. . The most commonly used method to detect FQNs residues in eggs in my country is Announcement No. 781 of the Ministry of Agriculture - 6...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/46
CPCG01N30/02G01N30/46
Inventor 王安波孙思杨梅汪俭
Owner 王安波
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