Method for distinguishing organic rice and non-organic rice by metabonomics technology

A technology of organic rice and metabolomics, applied in scientific instruments, material separation, analysis of materials, etc., can solve problems such as inability to effectively judge the quality of rice, and achieve simple and fast pretreatment methods, high convincing power, and simple pretreatment steps. Effect

Active Publication Date: 2018-04-20
HARBIN INST OF TECH
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Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that the existing detection means cannot make a comprehensive and effective judgment on the quality of rice, and to provide a method for distinguishing organic rice from non-organic rice using metabolomics technology

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  • Method for distinguishing organic rice and non-organic rice by metabonomics technology
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  • Method for distinguishing organic rice and non-organic rice by metabonomics technology

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specific Embodiment approach 1

[0022] Specific embodiment one: what this embodiment records is a kind of method utilizing metabonomics technology to distinguish organic rice and non-organic rice, described method is as follows:

[0023] After the organic rice samples and non-organic rice samples were pre-treated with organic solvents, the ultra-high performance liquid chromatography tandem quadrupole-time-of-flight high-resolution mass spectrometry method was used to realize the pretreatment of organic rice samples and non-organic rice samples. The separation and determination of the chemical components of the organic rice samples and non-organic rice samples were preprocessed by ultra high performance liquid chromatography tandem quadrupole-time-of-flight mass spectrometry (UHPLC-Q-TOF MS) raw data, Finally, the multivariate statistical analysis method Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA) model was used to distinguish organic rice from non-organic rice, and the factors that had a...

specific Embodiment approach 2

[0024] Embodiment 2: A method for distinguishing organic rice and non-organic rice using metabolomics technology described in Embodiment 1. The varieties of the organic rice sample and the non-organic rice sample are Daohuaxiang, Longjing and Songjaponica one or more of.

specific Embodiment approach 3

[0025] Specific embodiment three: a method for distinguishing organic rice and non-organic rice using metabolomics technology described in specific embodiment one, the pre-treatment specifically includes: crushing the organic rice sample and the non-organic rice sample, passing through 1mm Aperture sieve, and then the sieved organic rice samples and non-organic rice samples are mixed with organic solvents, ultrasonically, centrifuged, and passed through organic filter membranes to complete the pretreatment and obtain samples that can be tested on the machine.

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Abstract

The invention discloses a method for distinguishing organic rice and non-organic rice by a metabonomics technology, and belongs to the technical field of cereal quality detection. The method comprisesthe following steps: an organic rice sample and a non-organic rice sample are respectively pre-treated, chemical components in the pre-treated samples are separated and determined by a super high performance liquid chromatography series quadrupole-flight time high resolution mass spectrometry method, and the obtained original data of the two samples are pre-treated; and finally, the organic riceand the non-organic rice are distinguished by a multivariate statistical analysis method orthogonal partial least squares-distinguish analysis model, factors which have great influence on distinguishing are obtained by using an S curve map, and the substances are identified by an open-sourcing online database massbank. The analysis method can effectively distinguish the organic rice and the non-organic rice, can directly distinguish the classification conditions, has no need of relevant verification and analysis, and can obtain the conclusion; and the detection result is accurate and reliable.

Description

technical field [0001] The invention belongs to the technical field of grain quality detection, and in particular relates to a method for distinguishing organic rice from non-organic rice using metabolomics technology. Background technique [0002] Rice is one of the three major food crops in the world, and about 1 / 3 of the world's population uses rice as a staple food. Rice has long been the largest food crop produced and consumed, and most of its growers and consumers are in Asia. The improvement of rice quality is inseparable from the living standards and health of people in these areas. Rice is the main staple food of the vast majority of people in our country, and it is also the main export product of our country. With the continuous improvement of people's living standards, the requirements for the quality of rice are getting higher and higher, and organic rice has gradually entered the table of consumers. [0003] Organic rice refers to rice products that come from...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/86
CPCG01N30/02G01N30/72G01N30/8696
Inventor 马莺肖然李琳王荣春何胜华
Owner HARBIN INST OF TECH
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