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Preparation method of mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine

A technology of mycoplasma synovialum and dual inactivated vaccines, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacterial antigen components, etc., can solve the problem of increased weak chick rate in chicks and high morbidity and mortality in chicken flocks , egg production rate of laying hens, broiler body weight and feed conversion rate decrease, etc., to achieve good immunogenicity and prevent infection

Active Publication Date: 2014-01-01
兆丰华生物科技(南京)有限公司
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AI Technical Summary

Problems solved by technology

Mycoplasma gallisepticum and mycoplasma gallisepticum are often mixed with other bacteria or viruses, resulting in high morbidity and mortality in chicken flocks, which has brought huge economic losses to the chicken industry
[0003] The common feature of mycoplasma gallisepticum and mycoplasma synovialis is that the rate of weak chicks in chicks increases, the egg production rate of laying hens, the weight of broilers and the feed conversion rate all decrease. When drug treatment is used, it is easy to produce drug resistance Sex, the effect is not ideal
At present, the inactivated vaccine of Mycoplasma gallisepticum is blank in domestic reports, and in domestic farms, Mycoplasma gallisepticum and Mycoplasma gallisepticum are often co-infected, resulting in low feed utilization and egg production rates in chicken flocks. Expensive drug treatment costs make these two pathogens a bottleneck restricting the rapid development of the poultry industry

Method used

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  • Preparation method of mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine
  • Preparation method of mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1 Isolation and Identification of Mycoplasma Gallinarum HN01 Strain

[0021] 1. Disease data

[0022] The disease materials were aseptically collected the joint fluid and contents in the swollen hock joint cavity of chickens suspected of Mycoplasma synovialum infection.

[0023] 2 Isolation culture and morphological observation

[0024] The collected samples were isolated and cultured for mycoplasma, cultured in a 37°C incubator, inoculated into a new culture medium for subculture when the culture medium turned orange or yellow, culture smears were taken, Wright stained, and bacteria were observed under an oil microscope body shape. The color of the liquid medium turned yellow after 1 day of culture, and the colonies of the isolated bacteria were spherical or oval under the oil lens after Wright's staining. No bacterial growth on normal blood agar. The colony on the solid medium appeared as a small, smooth, dense colony, half sunken in the medium, and the co...

Embodiment 2

[0043]Example 2 Preparation of Mycoplasma gallisepticum and Mycoplasma gallisepticum dual inactivated vaccine

[0044] 1. Culture medium for Mycoplasma gallisepticum seedlings and preparation method thereof:

[0045] (1) Basic medium components: 44g of PPLO broth, 8g of yeast extract powder, 16g of glucose, 4ml of phenol red solution with a mass concentration of 0.4%, and 1460ml of deionized water.

[0046] (2) Preparation method of the finished medium: Autoclave the above (1) medium components at 121°C for 15 minutes, add filter-sterilized 22% pig serum and 1000 units / ml penicillin after cooling, and adjust the pH to 7. 7. Aseptically aliquot and store at 4°C for later use.

[0047] 2. Culture medium for chicken synovial bursa Mycoplasma seedlings and preparation method thereof:

[0048] (1) Basic medium components: 42g of PPLO broth, 12g of hydrolyzed milk protein, 6g of yeast extract powder, 0.6g of nicotinamide, 4ml of phenol red solution with a mass concentration of 0.4...

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Abstract

The invention relates to a preparation method of a mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine. The method comprises the steps as follows: a mycoplasma gallisepticum virulent CR strain and a mycoplasma synoviae HN01 strain which have good immunogenicity are inoculated on a proper culture medium for cultivation, so that a culture is acquired; and the culture is inactivated through a formaldehyde solution and then is mixed with an oil emulsion adjuvant and emulsified, so that the mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine is prepared. The mycoplasma gallisepticum and mycoplasma synoviae bivalent inactivated vaccine is used for preventing mycoplasma gallisepticum and mycoplasma synoviae diseases, and can realize immunization, prevent two pathogens at the same time and reduce the workload of immunization; and the prepared vaccine is stable in performance, good in immune effect, and more suitable for actual production of China.

Description

technical field [0001] The invention relates to a preparation method of a dual inactivated vaccine of mycoplasma gallisepticum and mycoplasma gallisepticum, belonging to the technical field of preparation of veterinary biological products and poultry disease prevention and control. Background technique [0002] Mycoplasma gallisepticum (MG) is a chronic respiratory infectious disease of chickens, characterized by rales, runny nose and air sacculitis in the respiratory tract. Chicken synovial bursa mycoplasma (MS) is mainly characterized by joint exudative synovial meningitis and tenosynovitis. Mycoplasma gallisepticum and mycoplasma gallisepticum are often co-infected with other bacteria or viruses, resulting in high morbidity and mortality in chicken flocks, which has brought huge economic losses to the chicken industry. [0003] The common feature of mycoplasma gallisepticum and mycoplasma synovialis is that the rate of weak chicks in chicks increases, the egg production ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/02A61P31/04C12N1/20C12R1/35
Inventor 丁美娟张海涛徐静
Owner 兆丰华生物科技(南京)有限公司
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