The invention relates to a vinegar residue deep processing technology, in particular to a method for producing protein feed by multi-strain solid state fermented vinegar residue, which increases the components of amino acid, vitamins, bioactivator, probiotics and the like in the protein feed obtained by using the microbial fermented vinegar residue and improves the palatability of the fermented feed. In the method for producing the protein feed by using the multi-strain solid fermented vinegar residue, a solid fermentation culture medium is used, the material-water ratio is 1:0.5-0.7, the fermentation temperature is 26-30 DEG C, the inoculating amount is 10-20 percent, the fermenting time is 48-120h; and the strain composition comprises oyster mushroom, Cryptococcus uniguttulatus, Saccharomyces cerevisiae and Cadida atilis. The protein feed is produced by using the microbial fermented vinegar residue, the invention ensures that the product contains more abundant amine acid, vitamins, bioactivator, probiotics and the like and improves the palatability of the fermented feed.