Microbial manure of containing rare earth elements
A microbial fertilizer and rare earth element technology, applied in organic fertilizers, fertilizer mixtures, fertilization devices, etc., can solve the problems of insufficient microbial fertilizer fertilizer efficiency and slow onset of microbial fertilizers, so as to improve survival rate and fertilizer efficiency and shorten the adaptation period , the effect of improving the survival rate
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Embodiment 1
[0011] Embodiment one: liquid bacterial fertilizer
[0012] Use "Askby's (Askby) culture solution (PH=7)" to obtain 20L high concentration (viable bacteria number reaches 9.6*10 8 each / mL) active bacterial liquid; add sterilized molasses 1Kg, bran (finely ground) 500g, food grade chitosan 4Kg, "phosphate-sodium dihydrogen phosphate" acid-base buffer solution (abbreviated as phosphate buffer) 2L , stirred for 2 hours in a sterile state, and then mixed with the above-mentioned 20L active bacterial liquid to become "bacterial fertilizer stock solution- 1 ". Divide into two parts, add 100mL 1% ReCl to one part 3 (light rare earth chloride), become "bacterial fertilizer stock solution- 1-RE ". These two liquid bacterial liquids (about 11 L each) were used for the control test respectively.
[0013] a. Comparison of bacterial cell survival rate during storage period. "Bacterial fertilizer stock solution- 1 " and " bacterial fertilizer stock solution- 1-RE "Store at room temper...
Embodiment 2
[0021] Embodiment two: solid bacterial fertilizer.
[0022] Inoculate the slant strain specimen of Sinorhizobium meliloti 042BM (Sinorhizobium meliloti) on YMA medium, keep at 28°C for 36h, wash the strain with sterile water, and shake well. 500mL of high-concentration "Bacterial Fertilizer Stock Solution-2" was finally obtained from multiple expansion cultures, which were divided into two parts (250mL each), and 10mL of 1% ReCl was added to one of them 3 , become "Bacterial Fertilizer Stock Solution-2-Re", and set aside.
[0023] Separately take peat (grass) charcoal without inclusions, crush, grind, and pass through 80 meshes to obtain peat subdivision. Soak the fine powder into 20% chitosan polymer solution, take it out after 2 hours, separate the entrained aqueous solution, dry and dehydrate, grind, and pass through 80 meshes to obtain another peat fine powder, whose porous structure or huge surface has been evenly spread A layer of chitosan, called "active solid chitosa...
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