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Method for purifying tea saponin by microbial fermentation

A technology of microbial fermentation and tea saponin, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of high safety production requirements, high methanol toxicity, and large usage, so as to shorten the production cycle, Low production cost and reduced usage

Active Publication Date: 2014-09-10
HANGZHOU TEA RES INST CHINA COOP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The organic solvent used in alcohol precipitation is generally methanol or ethanol, both of which are flammable and explosive, and methanol is highly toxic and requires high safety production
However, when ethanol is used, the general usage is very large, and there is a problem of large investment.

Method used

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  • Method for purifying tea saponin by microbial fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Pulverize the tea seed cake, pass through an 80-mesh sieve, weigh 150g of the pulverized tea seed powder, add it to 1500mL of 40°C water for ultrasonic extraction for 40 minutes, centrifuge to remove the precipitate, and prepare the supernatant for inoculation 50mL of good microbial fermentation yeast liquid, covered with gauze, let stand for 3 days, centrifuged to get the flocculation sediment, leached the sediment with 80% ethanol solution, centrifuged, the supernatant was evacuated and rotary evaporated, and the concentrated solution was sprayed After drying, the purity of tea saponin was measured to be (80%).

Embodiment 2

[0021] Example 2: Extract the pulverized Camellia oleifera seed powder with water for 40 minutes according to mass (g): volume (mL) = 1:10, filter to get the supernatant, and inoculate the prepared microbial fermentation yeast liquid with a volume ratio of 1:1 , let it stand for 2 days, take the supernatant, add fresh tea seed powder extract at a volume ratio of 1:1, and continue to ferment; such a cycle constitutes a semi-continuous fermentation. The flocs in the lower layer are centrifuged to obtain the precipitate and supernatant, and the supernatant is sterilized and then added to the rough extraction process of tea seed powder; the precipitate is leached with an ethanol solution with a mass concentration of 80%, centrifuged, and the supernatant is evacuated and rotary evaporated. The concentrated solution was spray-dried, and the purity of tea saponin was measured to be (85%).

[0022] The microorganisms used in the fermentation of Examples 1 and 2 to ferment the yeast li...

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Abstract

The invention discloses a method for purifying tea saponin by microbial fermentation, which is characterized by comprising the following steps: by using a water solution containing tea saponin as a fermentation substrate, inoculating a microbial flocculant generating bacterium capable of enabling the tea saponin to generate flocculating precipitate into the fermentation substrate, culturing until the tea saponin generates flocculation, carrying out centrifugal separation on the flocculating precipitate, extracting the tea saponin in the precipitate with ethanol to obtain the purified tea saponin ethanol solution, concentrating, and drying to obtain the refined tea saponin. The purification method can solve the problem of purification of tea saponin, and has the characteristics of safe production process and low production cost. The method has the advantages of simple technique, high operability and high product safety, and only uses the ethanol in the whole purification process. The tea saponin produced by the technique has favorable application prospects in the fields of daily use chemicals and health food.

Description

technical field [0001] The invention relates to a method for purifying tea saponin, in particular to a method for purifying tea saponin by microbial fermentation. Background technique [0002] Tea saponin, also known as tea saponin, is a kind of glycoside compound extracted from tea tree seeds. It is a kind of natural surfactant with good performance. It can be widely used in light industry, chemical industry, pesticide, feed, breeding, textile , oil extraction, mining, building materials and highway construction and other fields, manufacturing emulsifiers, detergents, pesticide auxiliaries, feed additives, crab and shrimp breeding protection agents, textile auxiliaries, oilfield foam agents, mining flotation agents and aerated concrete Foam stabilizer and concrete admixture - antifreeze, etc. [0003] Tea saponin belongs to triterpenoid saponin, has a bitter and pungent taste, stimulates the nasal mucosa to cause sneezing, the pure product is white fine columnar crystal, s...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/256C07H1/08
Inventor 梁慧玲王静杨秀芳涂云飞谭蓉孔俊豪
Owner HANGZHOU TEA RES INST CHINA COOP
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