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Domesticating and culturing method for thiobacillusthioparus applied to high-temperature aerobic composting

A technology of thiobacillus, domestication and culture, applied in microorganism-based methods, methods of using microorganisms, biochemical equipment and methods, etc. Metabolism, enhancing tolerance, achieving the effect of high-density culture

Active Publication Date: 2015-11-11
INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The composting environment is complicated, and there is a continuous high temperature period, which is not conducive to the growth of Thiobacillus thiaphus
Therefore, affecting its function

Method used

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  • Domesticating and culturing method for thiobacillusthioparus applied to high-temperature aerobic composting
  • Domesticating and culturing method for thiobacillusthioparus applied to high-temperature aerobic composting
  • Domesticating and culturing method for thiobacillusthioparus applied to high-temperature aerobic composting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] 1. Enrichment culture

[0056] 1) Thiobacillus thiophanus preserved on the slant ( Thiobacillus thioparus Beijerinck1904) was inoculated into the basal medium for activation, in a 500mL Erlenmeyer flask with a liquid volume of 250mL, and cultured at 28°C with a rotational speed of 120r / min for 3 days to obtain the activated initial Thiobacillus thulogenum bacteria liquid. The growth curve of Thiobacillus thiaphragms in basal medium is as follows: figure 1 shown.

[0057] The Thiobacillus thioexans ( Thiobacillus thioparus Beijerinck 1904) was purchased from LeibnizInstitut DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH; Curators of the DSMZ; number DSM5368.

[0058] The composition of the basal medium is: every liter of basal medium contains Na 2 HPO 4 1.2g, KH 2 PO 4 1.8g, MgSO 4 ·7H 2 O0.2g, (NH 4 ) 2 SO 4 0.2g, CaCl 2 0.04g, FeCl 3 0.02g, MnSO 4 0.02g, Na 2 S 2 o 3 12.0g, the balance is water, sterilized at 121°C for 20min, cool...

Embodiment 2

[0078] 1. Enrichment culture

[0079] Thiobacillus thiophanus preserved on the slant ( Thiobacillus thioparus Beijerinck1904) was inoculated into the basal medium for activation, in a 500mL Erlenmeyer flask with a liquid volume of 250mL, at 30°C, with a rotational speed of 150r / min, and cultured for 5 days to obtain the activated initial Thiobacillus thioexophrene bacteria liquid.

[0080] Thiobacillus thioexans in the present invention ( Thiobacillus thioparus Beijerinck 1904), deposited at LeibnizInstitut DSMZ-Deutsche Sammlung von Mikroorganismen und ZellkulturenGmbH; CuratorsoftheDSMZ; number DSM5368

[0081] Its composition of described basal medium comprises, Na 2 HPO 4 .1.0g, KH 2 PO 4 .1.5g, MgSO 4 .7H 2 O.0.1g, (NH 4 ) 2 SO 4 .0.1g, CaCl 2 .0.03g, FeCl 3 .0.01g, MnSO 4 .0.01g, Na 2 S 2 o 3 .10.0g, 1L of water, sterilized at 121°C for 20min.

[0082] Inoculate the activated initial Thiobacillus thioexhausen bacteria solution into the enrichment medium ...

Embodiment 3

[0101] 1. Enrichment culture

[0102] Thiobacillus thiophanus preserved on the slant ( Thiobacillus thioparus Beijerinck1904) was inoculated into the basal medium for activation, in a 500mL Erlenmeyer flask, with a liquid volume of 250mL, at 30°C, with a rotation speed of 150r / min, and cultured for 4 days to obtain the activated initial Thiobacillus thioexophrene bacteria liquid.

[0103] Thiobacillus thioexans in the present invention ( Thiobacillus thioparusBeijerinck 1904), deposited at LeibnizInstitut DSMZ-Deutsche Sammlung von Mikroorganismen und ZellkulturenGmbH; CuratorsoftheDSMZ; number DSM5368

[0104] Its composition of described basal medium comprises, Na 2 HPO 4 .1.5g, KH 2 PO 4 .2.0 g, MgSO 4 .7H 2 O.0.3g, (NH 4 ) 2 SO 4 .0.3g, CaCl 2 .0.05g, FeCl 3 .0.03g, MnSO 4 .0.03g, Na 2 S 2 o 3 .15.0g, 1L of water, sterilized at 121°C for 20min.

[0105] Inoculate the activated initial Thiobacillus thioexhausen bacteria solution into the enrichment medium...

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Abstract

The invention discloses a domesticating and culturing method for thiobacillusthioparus applied to high-temperature aerobic composting. The culturing method includes the thiobacillusthioparus enrichment culture step, the temperature domesticating step and the environment domesticating step and achieves high-density culture of the thiobacillusthioparus. The thiobacillusthioparus adapts to the temperature-rising process of composting, can tolerate high temperature and grow on a large scale in the complicated environment of compositing. According to the method, special enrichment mediums are used in the domesticating process, metabolism and nutrient utilization of the thiobacillusthioparus can be accelerated, high-temperature domesticating of the thiobacillusthioparus is facilitated, growth of the thiobacillusthioparus is facilitated, and enrichment is fast. The method solves the problems that the thiobacillusthioparus is not resistant to high temperature during aerobic composting and growing speed is low.

Description

technical field [0001] The invention relates to the technical field of microbial strain cultivation, in particular to a method for acclimating and cultivating Thiobacillus thiaphragms applied to high-temperature aerobic composting. Background technique [0002] High-temperature aerobic composting technology is the main way to realize the harmless, reduction and resource utilization of solid organic waste. However, there is always the problem of nitrogen loss and odor release in composting. [0003] The loss of nitrogen is mainly due to the high temperature and high pH in the composting process, which converts ammonium nitrogen into ammonia volatilization. Ammonia gas is the main component of the odor emitted in composting projects, and ammonia gas is corrosive to composting equipment after being dissolved in water. In addition, odorous substances in composting projects mainly include hydrogen sulfide, sulfur-containing organic matter and other substances. These gaseous su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/20C05F17/00C12R1/01
CPCY02W30/40
Inventor 顾文杰卢钰升解开治徐培智李文英李夏孙丽丽
Owner INST OF AGRI RESOURCES & ENVIRONMENT GUANGDONG ACADEMY OF AGRI SCI
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