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Method for producing alcohol by using stalks of jerusalem artichoke as raw material and adopting immobilized yeast fermentation of inulin

A technology for immobilizing yeast and Jerusalem artichoke, which is applied in microorganism-based methods, biochemical equipment and methods, fermentation and other directions to achieve the effects of low price, shortened adaptation period, and improved utilization of equipment

Inactive Publication Date: 2010-09-29
山西金绿禾燕麦研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a method for producing alcohol by immobilizing yeast and fermenting inulin by using Jerusalem artichoke stems as raw materials in order to solve the problems of producing alcohol from Jerusalem artichoke powder and utilizing inulin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1, a kind of method taking Jerusalem artichoke stalk as raw material immobilized yeast fermenting inulin to produce alcohol comprises the steps:

[0018] 1. The alcoholic yeast was expanded and cultivated step by step according to the inclined plane, shake flask, 50L fermenter, and 500L fermenter for 20 hours to obtain 300L yeast suspension.

[0019] 2. Wash the stems of Jerusalem artichoke, evenly cut them into 0.5cm segments, and then sterilize them with steam at 121° C. for 20 minutes to obtain the treated immobilized carrier.

[0020] 3. Add the treated immobilized carrier into the cultured 300L yeast suspension in an amount of 1:10, and culture with stirring at 30°C for 16 hours in a 500L fermenter to obtain 50Kg of alcohol-immobilized yeast.

[0021] 4. After squeezing the Jerusalem artichoke to obtain 16.1% (w / w) Jerusalem artichoke juice, adjust the pH to 3.5 after adding sulfuric acid, add 50Kg of alcohol stalk-immobilized yeast to 150L of the above-...

Embodiment 2

[0022] Embodiment 2, a kind of method taking Jerusalem artichoke stalk as raw material immobilized yeast fermenting inulin to produce alcohol comprises the steps:

[0023] 1. The alcoholic yeast was expanded and cultivated step by step according to the inclined plane, shake flask, 30L fermenter, 300L fermenter, and 3000L fermenter for 16 hours to obtain 2000L yeast suspension.

[0024] 2. Wash the Jerusalem artichoke stalks, cut them evenly into 0.75 cm sections, and then sterilize them with steam at 121° C. for 20 minutes to obtain the treated immobilized carrier.

[0025] 3. Add the treated immobilized carrier into the cultured 2000L yeast suspension in an amount of 1:8, and culture with stirring at 28°C for 18 hours in a 2000L fermenter to obtain 400Kg of alcohol-immobilized yeast.

[0026] 4. Jerusalem artichoke juice obtained after extraction of 15.4% (w / w), after adding sulfuric acid, adjust the pH to 3.6, add 1500L of above-mentioned treated artichoke juice to 400Kg alc...

Embodiment 3

[0027] Embodiment 3, a kind of method taking Jerusalem artichoke stalk as raw material immobilized yeast fermenting inulin to produce alcohol comprises the steps:

[0028] (1) Preparation of yeast seed liquid, yeast strains are enlarged and cultivated into yeast suspension;

[0029] (2) Preparation and pretreatment of the immobilized carrier: after the Jerusalem artichoke stalks are cleaned, cut into 1.0 cm segments and sterilized at high temperature;

[0030] (3) Preparation of immobilized yeast: soak the obtained sterilized Jerusalem artichoke stalk in the obtained yeast suspension at a weight ratio of 1:6, and culture at 28-30° C. for 16-24 hours to obtain the Jerusalem artichoke stalk immobilized yeast;

[0031] (4) Squeeze or extract the Jerusalem artichoke to obtain Jerusalem artichoke juice with a total sugar content of 13%, and adjust the pH of the Jerusalem artichoke juice to 3.5-3.8;

[0032] (5) Adding 30% of the carrier with the stem-immobilized yeast to the acidi...

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Abstract

The invention relates to a method for producing alcohol by using crop stalks as a raw material, in particular to a method for producing alcohol by using stalks of jerusalem artichoke as a raw material and adopting immobilized yeast fermentation of inulin, which solves the problems of producing alcohol by using jerusalem artichoke powder and utilizing the stalks of jerusalem artichoke. The method comprises the following steps of: 1, preparing yeast seed liquid; 2, preparing and pre-treating an immobilized carrier; 3, preparing immobilized yeast; 4, pressing or extracting the jerusalem artichoke to obtain jerusalem artichoke juice; and 5, adding the stalk-stem immobilized yeast into the acidified jerusalem artichoke juice according to the carrier addition amount of 10 to 30 percent for alcohol fermentation. By adopting the technique, the fermentation environment adaptation period of the yeast is shorted, the fermentation period is shorted, the sugar consumption when the yeast is propagated is lowered, and the utilization rate of equipment is high. Therefore, under conditions of not increasing personnel and equipment, the alcohol yield is increased, the fermentation production capability is improved, and the economic benefits are obvious.

Description

technical field [0001] The invention relates to a method for producing alcohol by using crop stalks as raw materials, in particular to a method for producing alcohol by using Jerusalem artichoke stalks as raw materials and immobilizing yeast to ferment inulin. Background technique [0002] Jerusalem artichoke is a crop with high and stable yield and wide application, and the main component of tubers is inulin. It is suitable for planting in most areas of our country. It is mainly used as a raw material for producing inulin, except that the tubers are directly eaten or processed into small foods. Using inulin as raw material can be used to extract inulin and process alcohol. Since the production of alcohol by using inulin does not have high energy-consuming processes such as liquefaction and saccharification of starchy raw materials, the production operation is simple and the advantages are obvious. The development of Jerusalem artichoke energy alcohol is an important measur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/85
CPCY02E50/16Y02E50/10
Inventor 田向东田伟民陈志刚
Owner 山西金绿禾燕麦研究所
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