27 results about "Sugar consumption" patented technology

The invention relates to a MnO2 in-situ coated nano diamond drug, which is prepared by the following steps: by taking nano diamond as a carrier, modifying the surface of the nano diamond with polylysine so as to improve the dispersity and provide amino groups, then connecting the nano diamond with glucose oxidase rich in carboxyl groups through an amide reaction, and then coating the surface of the nano diamond with MnO2 through KMnO4 reduction so as to obtain the MnO2 in-situ coated nano diamond drug. Finally, the chemotherapeutic drug DOX is loaded through coordination and electrostatic interaction. According to the nano diamond medicine, in a subacid tumor microenvironment rich in GSH, the wrapped MnO2 shell is subjected to a redox reaction to generate Mn < 2 + >, meanwhile, the MnO2 nano structure can trigger H2O2 in the tumor microenvironment to be decomposed to generate O2, tumor hypoxia can be relieved, GOx can be exposed, glucose in a tumor area can be effectively catalyzed to generate H2O2 under the aerobic condition, and therefore the tumor hypoxia can be relieved. Glucose consumption effectively cuts off nutrition sources of tumor cells, inhibits tumor cell proliferation and realizes tumor hunger treatment. According to the invention, chemotherapy, hunger therapy and chemical kinetics therapy are cooperated to efficiently and selectively kill tumor cells, and a remarkable anti-tumor effect is achieved.

The present invention discloses a method for preparing fuel ethanol from straw biomass and the fuel ethanol, belonging to the technical field of fuel ethanol production. It includes: dissolving and hydrolyzing straw and concentrated sulfuric acid to obtain a saccharified solution, separating out a sugar-rich solution in a simulated moving bed chromatography system, and then mixing it with Saccharomyces cerevisiae SEB3 for fermentation, and generating fuel ethanol through distillation. The method for preparing fuel ethanol from straw biomass provided by the present invention can obtain fermentable sugars under normal pressure and low temperature conditions by using concentrated acid hydrolysis, and has the remarkable advantages of low energy consumption, low toxic by-products, and no need to input cellulase. And Saccharomyces cerevisiae SEB3 is used for fermentation. This strain has the ability of co-fermenting all sugars, can realize the fermentation of all sugars, synchronously convert all sugars into ethanol, the sugar consumption efficiency reaches more than 90%, and the ethanol recovery rate reaches more than 80%, and it is applicable to industrial production.

The invention discloses a recombinant bacterium for producing L-lactic acid through co-utilization of glucose and xylose as well as a construction method and application of the recombinant bacterium. The invention relates to the technical field of biology, in particular to recombinant bacteria for producing L-lactic acid through co-utilization of glucose and xylose as well as a construction method and application. Methylglyoxal synthase, and / or pyruvate formate lyase, and / or fumarate reductase and / or L-lactic dehydrogenase in receptor Escherichia coli Rec1.0 are modified to obtain recombinant Escherichia coli, the recombinant Escherichia coli is fermented for 24 h, L-lactic acid is the only metabolite, and the yield reaches 2458 g / L; the consumption rate of xylose is 0.76 g / g DCW / h, the consumption rate of glucose is 0.69 g / g DCW / h, and the consumption rate of total sugar reaches 1.45 g / g DCW / h. The recombinant Escherichia coli constructed by the invention can realize efficient utilization of lignocellulose hydrolysate (containing glucose and xylose), and has a wide application prospect.

The invention belongs to the technical field of biochemical engineering, and discloses a method for producing squalene by inducing schizochytrium limacinum through a salt stress strategy and application, the method comprises the following steps: fermenting a schizochytrium limacinum strain for fermentation for 0-72 hours, adding 3g / L of NaCl into a fermentation medium, after 72 hours, adding NaCl into the fermentation liquid to 5g / L, continuing to ferment for 120 hours, measuring the sugar consumption every 12 hours in the fermentation process, and supplementing glucose to 80g / L. According to the method, the squalene yield of schizochytrium limacinum is remarkably increased through a salt stress strategy, an expensive inducer is prevented from being used, and the production cost is effectively reduced. 3 g / L of low-concentration salt is added in 0-72 h of the early stage of fermentation to serve as an adaptation period, so that schizochytrium limacinum grows rapidly and is induced to start an osmotic regulation mechanism, the concentration of NaCl is increased to 5 g / L in 72 h, at the moment, the schizochytrium limacinum enters a lipid accumulation period, and high-concentration NaCl is utilized to stress the schizochytrium limacinum to efficiently accumulate squalene.

The invention discloses a genetically engineered bacterium for producing D-pantothenic acid as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium takes DPA11A * 01 as an original strain, the copy number of ilvD, serA and glyA genes regulated by a promoter pTrc is increased on a genome of the original strain, an ldhA gene, an mgsA gene, a poxB gene and an adhE gene are knocked out, and an alsS gene from bacillus subtilis is over-expressed. After the strain for producing D-pantothenic acid is fermented in a 250 mL shake flask for 48 hours, the DPA yield reaches 7.84 g / L and is increased by 26% compared with an original strain. When the strain is fermented for 100 hours by using a 5L-fermentation tank, the highest yield is 72.4 g / L, the total sugar consumption is 500g when the fermentation is finished, the final fermentation volume is 3L, the sugar-acid conversion rate is 43.4% and is improved by 17.5% compared with that of an original strain DPA11A * 01, and the sugar-acid conversion rate is obviously improved.

The present invention discloses a process for separating sugar and acid using an eight-zone simulated moving bed. The eight-zone simulated moving bed system is composed of 16 chromatographic columns filled with H-type cation exchange resins, and every two columns form a zone with a total of eight zones, specifically separation zones (Zones II, III, VI, and VII), stationary phase regeneration zones (Zones I and V), and eluent regeneration zones (Zones IV and VIII). The mobile phase direction of the entire system is opposite to the column switching direction, and the flow rate is controlled by three inlets and three outlets to achieve continuous separation of three components (sulfuric acid, monosaccharides, and organic acids). The technical solution of the present invention can achieve a recovery rate of sulfuric acid and xylose higher than 98%, a mass purity of xylose and sulfuric acid higher than 99%, and a solvent consumption of only 0.09 mL of deionized water per 1 g of xylose produced.

The present application relates to the technical field of microbial fermentation culture, and specifically discloses a method for fermentatively producing glutamic acid, a culture medium and application thereof. The method for fermentatively producing glutamic acid adds catechol to seed culture medium and fermentation culture medium, starts to add flow-adding liquid one after complete consumption of the base sugar, and starts to add flow-adding liquid two when the acid concentration in the fermentation broth reaches 140-165 g / L; the flow-adding liquid one is a glucose solution containing propyl gallate, the concentration of glucose in the flow-adding liquid one is 68-72%, and the concentration of propyl gallate is 10-50 mg / 100 g; the residual sugar concentration in the fermentation broth is controlled to be less than 5 g / L by adding the flow-adding liquid one; and the flow-adding liquid two is a sorbitol solution, and the sorbitol concentration in the fermentation broth is controlled to be 0.5-4 g / L by adding the flow-adding liquid two. The method can improve the fermentation conversion rate and is suitable for industrial production.

Dealcoholisation techniques using physical methods are already known; however, there are currently no devices that can biologically lower alcohol content. The invention comprises equipment including at least one fermentation tank (1), provided with a plurality of probes and devices for controlling said fermentation, and at least one must reservoir tank (2), said tanks being connected by a transfer pump (4) and controlled by a control panel (18) that controls functions including oxidation, gas inflow, cooling, heating, stirring, transfer, volume measurement, temperature, density, sugar consumption, colour, acetic acid level.

The invention discloses a method for cultivating dendrobium officinale tender shoots under weak sugar and ventilation conditions, and belongs to the technical field of dendrobium planting. The formula of the dendrobium officinale tender shoot induction and proliferation liquid culture medium is optimized, and a certain amount of natural extracts and growth hormones are added into the improved MS culture medium to improve the growth promoting effect of the dendrobium officinale tender shoots; in the suspension culture process, a certain amount of air and carbon dioxide gas are additionally introduced into a culture medium, so that the nutrient absorption rate of a culture can be improved, the photosynthesis efficiency of protocorm is promoted, the growth speed of dendrobium officinale tender shoots is increased, the biomass accumulation is enhanced, the tender shoot induction rate and the proliferation coefficient of tissue culture are further improved, and the survival rate of the dendrobium officinale tender shoots is increased. Stable characters of the tender shoots are kept, and the content of active ingredients such as polysaccharide and amino acid in the tender shoots is improved; in addition, by means of ventilation co-culture, the using amount of sugar in the culture medium can be reduced, the culture cost and the pollution probability are reduced, the time needed by the dendrobium officinale tender shoot multiplication culture stage can be effectively shortened, and the culture cost is reduced. In addition, the dendrobium officinale tender shoots cultivated by the method also show a good analgesic effect, and have a very good market application prospect.

The invention relates to an alpha-glucosidase inhibitor and application thereof. The alpha-glucosidase inhibitor is vitamin C, dehydrogenated vitamin C, and 2, 3-diketone-L-gulonic acid. The influence of vitamin C, dehydrovitamin C and 2, 3-diketone-L-gulonic acid on alpha-glucosidase and HepG2 cell glucose consumption is researched through an in-vitro model, and the result shows that the vitamin C, dehydrovitamin C and 2, 3-diketone-L-gulonic acid can remarkably inhibit the activity of alpha-glucosidase and promote uptake and utilization of glucose by cells, so that the effect of improving insulin resistance is achieved.

The invention relates to synthesis and application of a polycaprolactone-glutamic acid block polymer, and provides polypeptide vesicles synthesized by initiating BLG polymerization by adopting PCL-NH2 through a self-designed catalytic system. Experiments prove that the constructed vesicles have dual functions, can significantly promote cell proliferation in the fermentation process of the cassava clear liquid, and can synchronously increase the sugar consumption rate at the same time. The system breaks through the traditional limitation in the field of biomedicine and biofuel application, the example of active regulation and control of microbial biosynthesis by polypeptide vesicles is shown for the first time, and a sustainable research platform is established for polypeptide nanoparticles and a biological refining technology.

The invention discloses a fusarium venanii strain with high substrate conversion rate and low RNA (Ribonucleic Acid) content and application of the fusarium venanii strain, and belongs to the fields of genetic engineering technology and food biotechnology. Compared with a wild strain, the fusarium venanii Fcdc has the advantages that the conversion rate of a gene Cdc42 on a genome from a substrate to thallus is increased to 67.06%, the conversion rate from the substrate to protein is increased to 26.01%, the sugar consumption for producing mycelia with the same dry weight is reduced by 60%-70%, the sugar consumption for producing mycelia protein with the same dry weight is reduced by 60%-70% compared with the wild strain, and the RNA content of the mycelia is reduced by more than 90% and is as low as 0.32% compared with the wild strain; the method has the advantages that the method is simple and convenient to operate, a heat treatment and nucleic acid removal step is not needed, the production efficiency and the safety quality of the fusarium venanii hypha protein are obviously improved, the production cost is reduced, and the fusarium venanii can be used for further developing genetic engineering modification work and cultivating fusarium venanii strains with high protein content and low RNA content.

The invention discloses a sweetening type composite floral compound additive and an application method thereof, and belongs to the technical field of food additives. The additive comprises erythritol, anhydrous citric acid, propylene glycol and a compound floral compound in a mass ratio of 5: 0.005: 0.01: (0.000175-0.004), wherein the compound floral compound is selected from at least three of alpha-ionone, beta-ionone, neryl acetate, nerol, nerolidol, terpinenol, farnerol and methyl dihydrojasmonate. By compounding three or more aroma substances and utilizing multiple aromas to synergistically influence taste perception, the effective sweetening effect can be realized on the premise of remarkably reducing the sugar consumption, and meanwhile, the taste comfort is improved. The method not only provides important reference for researching sweetening effects of different fragrances, has scientific research guidance value, but also provides a new strategy for realizing sugar reduction and gaining through aroma regulation and control for food industry, can respond to health requirements while maintaining flavor and taste, and has wide application prospect.

The application belongs to the technical field of biological resource utilization, and more particularly relates to a method for biological resource utilization of seafood waste. The method specifically comprises the following steps: 1) drying and crushing seafood waste for standby; 2) inoculating lactic acid bacteria into a fermentation medium, adding the seafood waste obtained in step 1) in an amount of 10-30 g / 100 mL, at a temperature of 40-45 DEG C, a rotation speed of 50-200 rpm, and fermenting for 36-72 h; when the sugar concentration of the fermentation broth is reduced to 10 g / L or less, start sugar supplementing, and the total sugar consumption in the fermentation process is not more than 150 g / L; 3) when the pH value of the fermentation broth is reduced to 4.5-5.0, the fermentation is completed, and the obtained precipitate is used for preparing chitin; and the filtered fermentation broth is used for extracting lactic acid or lactate. The lactic acid fermentation coupled with the seafood waste decalcification and deproteinization method established in the application does not need to add other organic nitrogen sources in the lactic acid fermentation process, and the fermentation medium and crab shells can not be sterilized, thereby effectively saving the treatment cost.

The invention discloses concanavalin A-like agglutinin / glucanase structural domain protein FvCLG and application thereof in improving the yield of fusarium venanii hypha protein, and belongs to the fields of genetic engineering technology and food biotechnology. Compared with a wild type strain, the fusarium venanii FG31 has the advantages that the FvCLG gene on a genome is deleted, ethanol is not generated in a fermentation tank for 72 hours, and the protein content is obviously increased (as high as 61.34%) and is increased by 22.41% compared with the wild type strain. Meanwhile, when the FG31 strain is fermented, the sugar consumption is obviously reduced, the conversion rate from glucose to biomass and the conversion rate from glucose to protein are respectively improved by 44.02% and 76.41%, the utilization rate of a substrate is obviously improved, the loss of a carbon source is reduced, and the production cost is reduced. The fusarium venanii can be used for further developing genetic engineering modification work to cultivate a fusarium venanii strain with high protein content.

This invention belongs to the field of fungal polysaccharide application technology, specifically relating to a method for preparing and applying polysaccharide from *Boletus globosum*. Crude polysaccharide was extracted from the fruiting body of *Boletus globosum* using hot water extraction, and purified using DEAE-52 cellulose column chromatography. Subsequently, by establishing an alcohol-induced liver injury model, it was found that NOP-1 significantly reduced ALT and AST levels, while simultaneously decreasing MDA content and increasing SOD activity and GSH levels, indicating that NOP-1 can inhibit lipid peroxidation, enhance the antioxidant defense capacity of hepatocytes, and maintain hepatocyte membrane integrity. A HepG2 cell IR model was constructed using a high-glucose, high-insulin combined induction method, determining the optimal dosage of NOP-1. ‑6 Treatment of HepG2 cells with mol / L insulin for 24 h successfully induced insulin resistance. Glucose consumption experiments in IR HepG2 cells demonstrated that NOP-1 significantly enhanced the glucose consumption capacity of insulin-resistant cells, exhibiting significant in vitro hypoglycemic activity.

The invention relates to the technical field of fermentation production, and discloses a fermentation production method of proline, which comprises the following steps: inoculating a slant culture medium with a proline producing strain, and culturing until the strain grows fully; eluting the strain in the slant culture medium by using sterile water to form a bacterial solution, and inoculating the bacterial solution into a seed culture medium for enlarged culture to obtain a seed solution; inoculating the seed solution into a fermentation culture medium for fermentation culture, and starting to supplement an amino acid mixture adjusting solution until fermentation is finished when the seed solution is cultured to an initial logarithmic growth stage and the OD value of fermentation liquor reaches 20-30; when the reducing sugar content of the fermentation liquid is smaller than or equal to 1.5%, a glucose aqueous solution is supplemented in a fed-batch mode to maintain the reducing sugar content to range from 1.0% to 1.5%, the fed-batch amount of the glucose aqueous solution is 25%-40% of the initial volume of the fermentation culture medium, and fermentation is finished after the supplemented sugar is consumed. The amino acid mixture adjusting liquid is supplemented in the fermentation process until the fermentation is finished, so that the sugar-acid conversion rate and the yield of the target product proline are improved.

The invention relates to the technical field of biological fermentation, in particular to a construction method and application of a pyruvate decarboxylase PDC1 deletion strain. The invention provides a construction method of a pyruvate decarboxylase PDC1 deleted strain, which comprises the step of inactivating or deactivating pyruvate decarboxylase PDC1 in a yeast strain in a physical mutagenesis, chemical mutagenesis or biological editing mode, and the acetic acid content in a fermented product of the strain constructed by the method is obviously increased by 10-35 times. The strain does not generate alcohol smell in the fermentation process, the sugar consumption is only half of that of a control group after fermentation is finished, the final growth biomass is not changed, and the single-cell protein content is increased. It shows that the deletion of PDC1 blocks the conversion route of pyruvic acid to acetaldehyde, and waste of carbon sources is reduced.

The invention belongs to the technical field of metabolic engineering, and particularly relates to a genetically engineered bacterium for producing 4-hydroxyisoleucine as well as a construction method and application of the genetically engineered bacterium. Corynebacterium glutamicum is used as a starting strain to replace a strong promoter e11 to regulate the expression of a 6-phosphofructokinase gene pfkA, point mutation is performed on a carbon metabolism transcription factor ramA and / or gntR, and heterologous expression is performed on an isocitrate dehydrogenase gene IDH regulated by the strong promoter e11, so that the genetically engineered bacterium is constructed. The engineering bacterium constructed through gene modification achieves the synergistic effect of rapid growth, advanced entering of a synthesis period and efficient sugar consumption, the overall period of the fermentation process can be remarkably shortened, the production efficiency can be improved, the yield of 4-HIL and the substrate utilization rate can be effectively increased, the yield of 4-HIL can reach 24.32 g / L when the strain is only fermented for 40 h, and the yield of 4-HIL can reach 24.32 g / L when the strain is fermented for 40 h. And the method has good practical application value and economic prospect.

The present invention relates to a preparation method and application of hemp seed polysaccharide. The preparation method of hemp seed polysaccharide comprises the following steps: (1) using hemp seed meal as raw material, extracting hemp seed crude polysaccharide HSP by water extraction and alcohol precipitation; (2) removing protein from the crude hemp seed polysaccharide, and separating it by ion exchange column chromatography to obtain HSP-II; (3) separating HSP-II by gel column chromatography to obtain HSP-IIa. The hemp seed polysaccharide prepared by the present invention can be used to lower blood sugar and improve the glucose consumption capacity of insulin-resistant cells.

The invention discloses a strain of low-sugar-consumption acid-resistant trametes robiniophila and application thereof.The strain of low-sugar-consumption acid-resistant trametes robiniophila is classified and named as trametes robiniophila HER-ZST-2023, the trametes robiniophila HER-ZST-2023 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on June 25, 2024, and the preservation number is CGMCC NO.41347. The strain of low-sugar-consumption acid-resistant trametes robiniophila has the advantages that the strain of low-sugar-consumption acid-resistant trametes robiniophila is high in yield and high in acid resistance; the low-sugar-consumption acid-resistant trametes robiniophila is applied to fermentation preparation of trametes robiniophila mycelia. The low-sugar-consumption acid-resistant trametes robiniophila can grow well in a low-sugar-consumption environment, the range of available culture medium raw materials is wider, the acid resistance is high, the trametes robiniophila can grow at a low pH value, the final hypha content can be kept, and the situation that growth is affected by acidic materials generated in the natural acidification or fermentation process of a culture medium is avoided; the pressure caused by environmental change can be resisted to a certain extent, stable growth and metabolism are maintained, the smooth proceeding of the fermentation process is guaranteed, and the risk of fermentation failure caused by environmental factors is reduced.

The invention relates to the technical field of food processing, in particular to a preparation method of low-sugar milk tea based on smell-taste interaction. According to the specific technical scheme, the method comprises the following steps: braising and soaking tea leaves with boiled water for 15-25 minutes, filtering, adding evaporated milk and cane sugar, uniformly stirring to prepare a milk tea base, and then adding water and edible essence. Through the synergistic effect of the added specific odor substances and the sucrose content, the sweetness perception is remarkably improved, and the actual sugar consumption is reduced. Meanwhile, aroma positively correlated with sweet taste perception is selected and is combined with low, medium and high sucrose concentrations, so that the optimal sensory experience is formed. And through a Pivot-CATA method and a consumer preference degree test, the improvement effect of different smells on the sweetness and the comprehensive preference degree under different sugar contents is verified.

The invention discloses a fermentation method of low-sugar Nanguo pear vinegar, which comprises the steps of raw material preparation, color protection, enzymolysis, component adjustment, alcoholic fermentation, acetic fermentation and post-treatment, the alcoholic fermentation adopts staged temperature control to promote sugar consumption, the residual sugar of the product is low, the flavor of Nanguo pears is reserved, and the product is rich in nutrition; the process is simple and suitable for industrial production.

The application discloses a concanavalin A-like lectin / dextranase domain protein FvCLG and application thereof in improving production of fusarium venenatum mycelial protein, and belongs to the field of genetic engineering technology and food biotechnology. Fv CLG Compared with the wild type, the fusarium venenatum FG31 lacks a gene on the genome, does not produce ethanol on a fermenter for 72 h, has a significantly improved protein content (up to 61.34 %), and is 22.41 % higher than the wild type strain. Meanwhile, the sugar consumption of the FG31 strain during fermentation is significantly reduced, the glucose-to-biomass conversion rate and the glucose-to-protein conversion rate are respectively increased by 44.02 % and 76.41 %, the substrate utilization rate is significantly improved, the carbon source loss is reduced, and the production cost is reduced. The fusarium venenatum can be further used for gene engineering modification work, and a fusarium venenatum strain with high protein content is cultivated.

The application discloses a strain of Fusarium venenatum with high substrate conversion rate and low RNA content and an application thereof, and belongs to the field of genetic engineering technology and food biotechnology. Cdc42 Compared with the wild type, the Fusarium venenatum Fcdc has the following advantages: the substrate conversion rate is increased to 67.06%, the substrate to protein conversion rate is increased to 26.01%, the sugar consumption for producing the same dry weight of mycelium is reduced by 60%-70%, the sugar consumption for producing the same dry weight of mycelium protein is reduced by 60%-70% compared with the wild type, the RNA content of the mycelium is reduced by more than 90% compared with the wild type strain, is as low as 0.32%, and the step of removing nucleic acid by heat treatment is not needed, so that the production efficiency and safety quality of the mycelium protein of the Fusarium venenatum are significantly improved, the production cost is reduced, the Fusarium venenatum can be further used for genetic engineering modification work, and a Fusarium venenatum strain with high protein content and low RNA content is cultivated.

The invention relates to the technical field of food, in particular to a Suzhou-style ghee mooncake and a preparation method of the Suzhou-style ghee mooncake. The invention relates to a Suzhou-style ghee mooncake, which comprises an oil crust, pastry, stuffing and hand flour, and is characterized in that the oil crust comprises the following raw materials in parts by weight: 48.43% of wheat flour, 0.23% of salt, 8.64% of white granulated sugar, 4.76% of fructo-oligosaccharide, 3.61% of a humectant, 24.53% of drinking water, 9.48% of edible oil and 0.32% of potassium sorbate; the bakery shortening comprises the following raw materials in parts by weight: 69.83% of wheat flour and 34.98% of edible oil; the stuffing is prepared from the following raw materials in parts by weight: 2.44% of unsalted butter, 12.04% of walnut kernels, 11.23% of wheat flour, 0.38% of salt, 19.09% of sweetened bean paste stuffing, 8.52% of white granulated sugar, 7.74% of soybean oil, 12.47% of pine nuts, 16.89% of macadamia nuts, 7.73% of rose thick slurry and 1.46% of fructo-oligosaccharide; the hand flour is wheat flour. Compared with the prior art, the Suzhou-style ghee mooncake and the preparation method thereof have the advantages that the oil consumption and the sugar consumption are reduced, and the stuffing is rich, so that the taste of the Suzhou-style mooncake is improved, and the Suzhou-style mooncake is deeply loved by consumers.

A method for producing baked goods with less added sugar without compromising the taste, sweetness and flavor of the baked goods. The method includes adding the enzyme alternansucrase in the dough-making process and may reduce the amount of added sugar in various baked goods by 50% without compromising taste and flavor. Addition of enzyme alternansucrase also maintains / improves certain key sensory qualities of the baked goods.