Phellinus igniarius phenol extract with blood sugar reducing activity and preparation and application of phellinus igniarius phenol extract

A technology of Phellinol and extract, applied in the field of Phellinol extract, can solve the problem of high cost, and achieve the effect of low cost, high preparation efficiency and good blood sugar lowering effect

Pending Publication Date: 2020-05-05
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is only one article on phenolics, which shows that the three compounds of protocatechualdehyde, naringenin and baicalein have the effect on HepG 2 Cellular glucose consumption has a significant promotion effect, but it is expensive to obtain these three pure compounds

Method used

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  • Phellinus igniarius phenol extract with blood sugar reducing activity and preparation and application of phellinus igniarius phenol extract
  • Phellinus igniarius phenol extract with blood sugar reducing activity and preparation and application of phellinus igniarius phenol extract
  • Phellinus igniarius phenol extract with blood sugar reducing activity and preparation and application of phellinus igniarius phenol extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] See the process flow figure 2 , Phellinus japonica cultivation fruiting body → vacuum drying at 45°C → crush and sieve to 50 mesh → weigh 50g and add 1:10 (solid-to-liquid ratio, w / w), 60% (w / w) ethanol aqueous solution → ultrasonic assisted extraction, The extraction power is 80W, the time is 20in, and the number of extractions is 3 times→5000r / min centrifuge to remove residue→combine the supernatant liquid→rotary evaporation, 50℃, vacuum degree 0.1Mpa, concentrate to 1 / 5 of the original volume→freeze drying→ Phellinus ethanol extract (EE) → add 200mL pure water for ultrasonic suspension, add the same volume of petroleum ether to extract twice, and collect petroleum ether extract (PET) → add the remaining phase to the same volume of ethyl acetate to extract twice, combine Collect the ethyl acetate extract phase (EtOAc) → add the same volume of n-butanol to the remaining phase and extract twice, combine and collect the n-butanol extract phase (n-BuOH) → collect the abo...

Embodiment 2

[0066] Cultivate the fruit body of Phellinus japonica → vacuum dry at 45°C → crush and sieve to 50 mesh → weigh 50g and add 1:20 (solid-to-liquid ratio, w / w), 70% (w / w) ethanol aqueous solution → ultrasonic assisted extraction, extract The power is 90W, the time is 30min, and the number of extractions is 3 times→5000r / min centrifuge to remove residue→combine the supernatant liquid→rotary evaporation, 50℃, vacuum degree 0.1Mpa, concentrate to 1 / 5 of the original volume→freeze drying→mulberry Yellow ethanol extract (EE) → add 300mL pure water for ultrasonic suspension, add the same volume of petroleum ether to extract twice, and collect petroleum ether extract (PET) → add the remaining phase to the same volume of ethyl acetate to extract twice, combine and collect Ethyl acetate extraction phase (EtOAc) → add the same volume of n-butanol to the remaining phase and extract twice, combine and collect the n-butanol extraction phase (n-BuOH) → collect the above EtOAc phase → concentra...

Embodiment 3

[0069]Cultivate the fruit body of Phellinus japonica → vacuum dry at 45°C → crush and sieve to 60 mesh → weigh 50g and add 1:30 (solid-to-liquid ratio, w / w), 80% (w / w) ethanol aqueous solution → ultrasonic assisted extraction, extract The power is 100W, the time is 30min, and the number of extractions is 3 times→5000r / min centrifuge to remove slag→combine the supernatant liquid→rotary evaporation, 50℃, vacuum degree 0.05Mpa, concentrate to 1 / 5 of the original volume→freeze drying→mulberry Yellow ethanol extract (EE) → add 300mL pure water for ultrasonic suspension, add the same volume of petroleum ether for extraction 3 times, and collect petroleum ether extract (PET) → add the remaining phase to the same volume of ethyl acetate for extraction 3 times, combine and collect Ethyl acetate extraction phase (EtOAc) → add the same volume of n-butanol to the remaining phase and extract 3 times, combine and collect the n-butanol extraction phase (n-BuOH) → collect the above EtOAc phase...

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Abstract

The invention relates to a phellinus igniarius phenol extract with blood sugar reducing activity, a preparation method for the phellinus igniarius phenol extract and application of the phellinus igniarius phenol extract. According to the preparation method, an ethyl acetate extract with relatively high phenol purity (not lower than 70%) is obtained through ultrasonic ethanol aqueous solution assisted extracting and solvent branched extracting and mainly comprises substances such as osmudacetone, hispidin, davallialactone, 2,5-bis(4,7-dihydroxy-8-methyl-2-oxo-2H-chromen-3-yl)cyclohexa-2,5-diene-1,4-dione, hypholomine B and inoscavin A. The phellinus igniarius phenol extract disclosed by the invention is high in phenol content, has a remarkable inhibiting action on alpha-glycosidase, has anobvious promoting action on glucose consumption of cells HepG2 and has good blood sugar reducing activity and potential diabetes preventing and treating effect. The preparation method is relatively simple in process procedure, free of a fine separating step, relatively high in preparation efficiency and relatively low in cost and is applicable to subsequent pilot trial and industrial production.

Description

[0001] (1) Technical field [0002] The invention relates to a phyllinoid extract with hypoglycemic activity, a preparation method and application thereof. [0003] (2) Background technology [0004] Diabetes mellitus (DM) is a chronic endocrine and metabolic disease characterized by carbohydrate, fat, protein, water, and electrolyte disorders caused by absolute or relative insufficient secretion of insulin in the body, and is related to genetic factors and various environmental factors. Its typical manifestations are characterized by "three more and one less" - polydipsia, polyphagia, polyuria, weight loss, and increased blood sugar. [0005] Diabetes mellitus (DM) is a chronic endocrine and metabolic disease characterized by carbohydrate, fat, protein, water, and electrolyte disorders caused by absolute or relative insufficient secretion of insulin in the body, and is related to genetic factors and various environmental factors. Its typical manifestations are characterized b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/07A61P3/10A23L33/105
CPCA23V2002/00A61K36/07A61K2236/17A61K2236/333A61K2236/39A61K2236/51A61K2236/53A23L33/105A61P3/10A23V2200/328A23V2250/208
Inventor 杨开张酥蔡铭关荣发孙培龙
Owner ZHEJIANG UNIV OF TECH
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