Application of neohesperidin in preparing of diabetes preventive treatment medicines

A technology of neohesperidin and diabetes, which is applied in the field of medicine, can solve the problems such as no reports on the effect of neohesperidin, achieve good clinical application prospects, reduce adverse reactions, and improve the effect of glucose and lipid metabolism

Inactive Publication Date: 2012-11-14
ZHEJIANG UNIV
View PDF2 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The effects of naringin and hesperidin on glucose and lipid metabolism have been reported in the literature, and their mechanism of

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of neohesperidin in preparing of diabetes preventive treatment medicines
  • Application of neohesperidin in preparing of diabetes preventive treatment medicines
  • Application of neohesperidin in preparing of diabetes preventive treatment medicines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] One of the sources of neohesperidin in the present invention is obtained from the white cortex of the citrus plant Huyou, and its preparation method and results are as follows:

[0023] Preparation of the crude extract powder of the macroporous resin of the white cortex of Huyou: 16 g of the dried white cortex of Huyou was extracted twice with 80% ethanol (solid-to-liquid ratio 1:25) ultrasonically (40°C), centrifuged at 4000 rpm for 20 min, Combine the two supernatants and evaporate to dryness on a rotary evaporator to obtain an extract, which is dissolved in ddH 2 In O, about 400 mL of D101 macroporous resin was used, the column bed volume (BV) was 20 mL, and the sample loading flow rate was 2 mL / min. The D101 macroporous resin after sample loading was first washed with water for 4 BV to remove impurities, and then naringin and Neohesperidin, wash 4 BV for each gradient, the elution flow rate is 2 mL / min, combine 10%-60% eluent, and evaporate to dryness on a rotary e...

Embodiment 2

[0028] The hypoglycemic activity of human hepatocytes was studied on the natural source neohesperidin extracted in Example 1, and it was found that neohesperidin can reduce the glucose concentration in the cell culture medium, and its glucose consumption is significantly higher than that of the control group. The experimental methods and results are as follows:

[0029] Human HepG2 hepatocytes were cultured in high-glucose DMEM containing 10% fetal bovine serum at 37°C and 5% CO 2 Incubate in a cell incubator, replace with fresh culture medium every other day, and passage once every 2 to 3 days. During the experiment, HepG2 cells were seeded in 96-well plates, and blank control wells without cells were set. After the cells grow to 80-90% confluence, discard the original medium, wash twice with PBS buffer, replace with serum-free 1640 culture medium containing 0.2% BSA, and add test substances in groups.

[0030]Set up DMSO solvent control group, metformin positive control gr...

Embodiment 3

[0034] The present invention finds that neohesperidin can obviously up-regulate the phosphorylation level of glucolipid metabolism-related signal proteins after acting on liver cells. The experimental methods and results are as follows:

[0035] Human liver HepG2 cells were inoculated in culture dishes, respectively set up DMSO solvent control group, metformin control group (final concentration 1 mmol / L), neohesperidin and naringin 1 μg / ml treatment groups, and neohesperidin and naringin at 0.5 μg / ml each, the cells were collected after 24 hours of treatment, and the phosphorylated protein levels of the target proteins AMPK and ACC were detected by Western blotting. The same experiment was repeated 3 times.

[0036] Protein extraction and concentration detection: The collected cell suspension was transferred to a centrifuge tube and centrifuged at 1000 rpm for 5 min. Remove the culture medium, wash the cells twice with cold PBS buffer, store at -80°C or directly lyse. Whe...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides application of neohesperidin in preparing of diabetes preventive treatment compound medicines or health care products. Neohesperidin is extracted and separated from citrus grape fruit tengyuch euonymus bark layers and is subjected to glucose and lipid metabolism biological activity research, and just as naringin, neohesperidin can obviously increase hepatic cell glucose consumption, improve phosphorylation levels of glucose and lipid metabolism related protein adenosine monophosphate activated protein kinase (AMPK) and lipid synthesized key protein activated calcium carbonate (ACC) and cooperate to reduce triglyceride level in lipopexia hepatic cells molded by free fatty acid, has effects of prompting reduction of blood sugar and blood lipid levels, and effect concentration of neohesperidin is far lower than that of positive medicine metformin. Neohesperidin can serve as ancillary drugs or health care products to be used for preventive treatment of diseases related to glucose and lipid metabolism disorder.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to the application of neohesperidin, a flavonoid compound from natural sources, or a composition thereof in preventing and treating diseases related to abnormal glucose and lipid metabolism, and in particular relates to the use of neohesperidin in the preparation of a single drug or a compound drug for preventing and treating diabetes Or application in health products. Background technique [0002] Abnormal glucose and lipid metabolism is very common in middle-aged and elderly people, and it is a common feature of metabolic syndromes such as diabetes, obesity, insulin resistance, and nonalcoholic fatty liver disease (NAFLD). Improving abnormal glucose and lipid metabolism is of great significance for preventing and delaying diabetes, hyperlipidemia, fatty liver, hypertension, angina pectoris, and stroke. Metformin is currently the first-line therapeutic drug for the treatment of diabetes, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K31/7048A61K45/00A61P3/10A23L1/29A23L33/00
Inventor 吴昊姝杨波何俏军陈昆松李鲜孙崇德
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap