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Method for improving production conversion rate of erythritol and shortening fermentation period

An erythritol and fermentation cycle technology is applied in the fields of improving the conversion rate of erythritol production and shortening the fermentation cycle, and can solve the problems of low erythritol conversion rate, single fermentation condition control, increased probability of miscellaneous bacteria, and the like, Achieve the effect of shortening the fermentation cycle, simple operation, increasing concentration and conversion rate

Pending Publication Date: 2022-02-11
康彤(上海)生物研发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conversion rate of erythritol produced by fermentation with yeast and glucose as a carbon source is low, and due to the long fermentation cycle, the probability of conversion of polluting bacteria increases during the fermentation process
[0004] At present, in the process of producing erythritol by microbial fermentation, most of the research is to increase the content of erythritol from single factors such as strain improvement, medium optimization, culture temperature, pH, dissolved oxygen, etc., but these fermentation conditions The control of the method is relatively simple, and the real-time adjustment according to the growth characteristics of the hyperosmotic yeast cannot be realized during the fermentation process to achieve the optimal growth and metabolism environment of the strain. However, the growth of the strain in the microbial fermentation method is a dynamic process, and the fermentation process requires Real-time online feedback regulation of a fermentation process according to its cell growth and metabolism, so as to achieve the highest conversion rate of erythritol and the shortest fermentation cycle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for improving the production conversion rate of erythritol and shortening the fermentation period, the specific steps are as follows:

[0020] (1) Cultivate Yarrowia lipolytica in a shaker to obtain OD 600nm 10 yeast seed liquid.

[0021] (2) The fermentation medium was sterilized at 105° C. for 15 minutes, and after cooling, the yeast seed liquid was inoculated in the fermentation medium for cultivation, the inoculum size was 10%, and the initial pH of fermentation was 6.8; the fermentation stage passed the adjustment of pH and OD Combined regulation makes RQ in the stage of increasing trend of 0.2-1.1, and controls the fermentation parameters: temperature 30±0.5°C, dissolved oxygen DO 2-30%, tank pressure 0.02-0.10MPa, speed 100-400rpm, pH3.5, so that the bacterial cells Density OD 600nm Increase to 35; the formula of the fermentation medium is: glucose 300g / L, yeast powder 5g / L, diammonium hydrogen phosphate 10g / L, corn steep liquor (Baume degree 19.5) 10g...

Embodiment 2

[0025] A method for improving the production conversion rate of erythritol and shortening the fermentation period, the specific steps are as follows:

[0026] (1) Cultivate Yarrowia lipolytica in a shaker to obtain OD 600nm 12 yeast seed liquid.

[0027] (2) The fermentation medium was sterilized at 105° C. for 15 minutes, and after cooling, the yeast seed liquid was inoculated in the fermentation medium for cultivation, the inoculum size was 10%, and the initial pH of fermentation was 6.3; the fermentation stage passed the adjustment of pH and OD Combined regulation makes RQ in the stage of increasing trend of 0.2-1.5, and controls the fermentation parameters: temperature 30±0.5°C, dissolved oxygen DO 2-30%, tank pressure 0.02-0.10MPa, speed 100-400rpm, pH4.0, so that the bacterial cells Density OD 600nm increase to 55; the formula of the fermentation medium is: glucose 290g / L, yeast powder 10g / L, diammonium hydrogen phosphate 5g / L, corn steep liquor (Baume degree 19.5) 5g / ...

Embodiment 3

[0031] A method for improving the production conversion rate of erythritol and shortening the fermentation period, the specific steps are as follows:

[0032] (1) Cultivate Yarrowia lipolytica in a shaker to obtain OD 600nm 15 yeast seed liquid.

[0033] (2) The fermentation medium was sterilized at 105° C. for 15 minutes, and after cooling, the yeast seed liquid was inoculated in the fermentation medium for cultivation, the inoculum size was 10%, and the initial pH of fermentation was 6.0; the fermentation stage passed the adjustment of pH and OD Combined regulation to keep RQ in the stage of increasing trend of 0.2-1.0, control fermentation parameters: temperature 30±0.5°C, dissolved oxygen DO 2-30%, tank pressure 0.02-0.10MPa, speed 100-400rpm, pH3.7, so that the bacterial cells The density OD600nm increased to 45; the formula of the fermentation medium was: glucose 280g / L, yeast powder 1g / L, diammonium hydrogen phosphate 1g / L, corn steep liquor (Baume degree 19.5) 1g / L, p...

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Abstract

The invention discloses a method for improving the production conversion rate of erythritol and shortening the fermentation period, and belongs to the technical field of biology. According to the method for improving the erythritol production conversion rate and shortening the fermentation period, saccharomycetes are used as fermentation strains, glucose is used as a carbon source, and the concentration, activity and sugar consumption rate of thalli are maintained in the optimal range through combined control of pH, OD and RQ in the fermentation process, so that the fermentation period is shortened, and the concentration and the conversion rate of erythritol are improved. The method is simple to operate, low in cost and short in period, and the conversion rate is remarkably improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for increasing the conversion rate of erythritol production and shortening the fermentation cycle. Background technique [0002] Erythritol, also known as butylene glycol, the molecular formula is C 4 h 10 o 4 , widely exists in the natural environment, such as melons, seaweed, mushrooms, grapes and other fruits and vegetables. Erythritol is a new type of functional sugar alcohol with good physical and chemical properties such as low melting point, good sweetness coordination, low calorie, low hygroscopicity, more heat absorption when dissolved, and high stability to acid and heat. As a functional sugar alcohol with good physical and chemical properties and biological activity, erythritol is widely used in food and beverage, dairy products, daily chemical and pharmaceutical industries. [0003] Erythritol was first extracted from algae, moss and some grasses, but its cos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P7/18C12R1/645
CPCC12N1/16C12P7/18
Inventor 张金祥张静超张雷达
Owner 康彤(上海)生物研发有限公司
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